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Journal : Hasanuddin Journal of Animal Science (HAJAS)

Movement Patterns of Bali Bull (Bos sondaicus) Sperms After Supplementation of Micronutrients Zn, Selenium, and Vitamin E Arisanti, N.; Yusuf, M.; Toleng, A. L.
Hasanuddin Journal of Animal Science (HAJAS) Vol. 7 No. 1 (2025)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v7i1.33344

Abstract

In ruminant livestock, it is very important to pay attention to the adequacy of micro minerals and vitamins, considering that minerals and vitamins are absolute and must be present in the feed so that the health and productivity of the livestock is not disturbed. To produce quality semen, vitamin E, selenium and zinc are needed. These micronutrients play a role in the spermatogenesis process which will have an impact on the quality of spermatozoa produced by male livestock. The aim of this study was to determine the effect of supplementation of micronutrient on sperm movement pattern of Bali bull. This study was used two Bali bull aged 4-5 years with a body weight of 300-350 kg. The study was divided into two periods of treatments and each period was carried out for three weeks. In the first period (control), experimental Bali bull was fed natural grasses and concentrate (1% of body weight). Furthermore, in the second period (treatment period), the feed was similar as the first period with the addition of a mixture of minerals (Zn and Se) and vitamin E. The volume, color, pH, viscosity and movement pattern of the bull's sperm were recorded. Likewise, sperm concentration, motility, viability and abnormality were measured. Fresh semen was diluted with Andromed® accordingly before subsequent processing. Data were analyzed using paired student t-test. The results of the study showed that the macroscopic quality of fresh semen of Bali bull improved after supplementation of micronutrient. The movement pattern of spermatozoa after administration of micronutrient supplements did not have a significant effect (P>0.05). It can be concluded that micronutrient supplementation in Bali bulls can improve macroscopic evaluation, abnormality, viability, concentration and maintained spermatozoa movement patterns. Keywords: Bali bull, spermatozoa, micronutrient, semen quality, and movement
Correlation Between Blood Metabolite Profile and Spermatozoa Quality in Bali Semen by Micronutrient Addition Dondatu, E.; Toleng, A. L.; Yusuf, M.
Hasanuddin Journal of Animal Science (HAJAS) Vol. 7 No. 1 (2025)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v7i1.34068

Abstract

The hematological profile and blood metabolite status are indicators of the physiological condition of livestock. This study aimed to determine the correlation between blood metabolite profiles (blood glucose and cholesterol) and sperm quality (motility and abnormality) in fresh Bali bull semen. Blood collection and semen sampling were conducted twice a week for 5 weeks. The collected blood and fresh semen were evaluated from two treatments: before and after the addition of micronutrients (including Zn, Se, and Vitamin E) in the feed. The parameters observed in this study were blood metabolite profiles (blood glucose and cholesterol levels) and sperm quality (percentage (%) motility and % abnormality of spermatozoa) before and after the addition of micronutrients. The data obtained were analyzed using simple linear regression and correlation tests. The results of this study showed that both treatments had a correlation between blood metabolite profiles and sperm quality in fresh Bali bull semen. Blood glucose levels correlated with sperm motility in fresh Bali bull semen, and good sperm motility values were obtained in all treatments before and after micronutrient administration in the feed. Cholesterol levels correlated with sperm abnormalities in fresh Bali bull semen, and all treatments before and after micronutrient addition in the feed showed abnormality values above the standard value. The blood metabolite profile (glucose and cholesterol) correlates with spermatozoa quality (motility and abnormalities) in fresh semen from Bali bull, where the addition of micronutrients in feed can increase spermatozoa motility but is not effective in suppressing the decrease in spermatozoa abnormalities. Keywords: Blood metabolite, blood sugar, cholesterol, spermatozoa quality