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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Miftahul Ilmi
Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Keragaman Isolat Khamir Osmofilik pada Madu Hutan dari Sulawesi Tengah Ditinjau Menggunakan Teknik RAPD Ferinta Rahmayanti; Miftahul Ilmi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 3 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2019.36.3.988

Abstract

Honey is a sweet substance with high sugar content produced by bees from flower nectar. Osmophilic yeast is one of organisms that can grow in honey because their ability to adapt in high osmotic pressure environment. Twenty-seven osmophilic yeast isolates were obtained from 11 forest honey samples to this research. This research objective is to determine the diversity of yeast isolates from genotypic characterization. Genotypic characterization was done using DNA fingerprint analysis using the RAPD PCR technique. This research used 2 types of primers, namely CDU primer (5′-GCGATCCCCA-3 ′) and M13 primer (5′-GAGGGTGGCGGTTCT GAGGGTGGCGGTTCT-3 ′). Data in the form of electrophoregram are represented in table n (yeast strain) x t (character units) using binary scoring type. The data were analyzed using a numerical taxonomy method with similarity (OTU) and determined using Jaccard coefficient (SJ) in 70% of IS limit. The results of this research showed that 27 yeast isolates were divided into 2 clusters and 10 outliers with a correlation coefficient (r) of 0.924624
Penapisan Enzim Invertase dari Khamir Asal Nektar dan Madu Hutan Nur Wahidatun Ni’matun Hasanah; Miftahul Ilmi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 37, No 3 (2020)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2020.37.3.1088

Abstract

Yeast is unicellular eukaryotic microorganism and one of the main resources in of organic compound. The natural compounds produced by yeasts have high commercial value; one of the examples is enzyme. Enzymes are widely used in industrial production processes. The invertase enzyme has the ability to catalyze sucrose, and hydrolyze it into equimolar monosaccharides, D-glucose and D-fructose mixtures called invert sugar. Invertase itself is commonly found in various parts of plants wich has high carbon content. Honey and nectar are compounds that have high carbon contents. Very high glucose and fructose contents show high levels of sucrose hydrolysis, so it is possible to have high levels of invertase enzymes in honey and nectar. This study aims to perform screening of invertase enzyme from yeasts that isolated from the nectar and forest honey and to find out the optimum temperature and pH for the enzyme production. This research was conducted by isolating yeast contained in wild honey and flowers nectar. Then conducted by qualitative test with selective medium and benedict test method. Quantitative test of intervase unit activity measured by DNS method with OD 540 nm. Protein concentration measured by Lowry Method with OD 650 nm. From 47 isolats 16 isolates able for invertase enzymes production. Yeast isolat SG 3.2 unit activity is 0.222 U/ml and specific activity is 7.07 U/mg. Optimum production pH and temperature are 5 and 25°C, respectively.
Co-Authors Ferinta Rahmayanti Nur Wahidatun Ni’matun Hasanah