Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Molekul: Jurnal Ilmiah Kimia
Agus Hery Susanto
Faculty of Biology, Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Materials Science & Nanotechnology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
RAPD Profile Of Winged-Bean [Psophocarpus tetragonolobus (L.)DC] Mutated Using Ethyl-Methane Sulphonate Fajri Hartanti; Adi Amurwanto; Agus Hery Susanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 1 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.326

Abstract

Genetic diversity of winged-bean [Psophocarpus tetragonolobus (L.) DC] can be produced by induced mutation with EMS (ethyl-methane sulphonate). To identify the genetic diversity after EMS application on winged-bean, molecular analysis using RAPD (Randomly Amplified Polymorphic DNA) technique was carried out. This study aims to identify RAPD profile of winged-bean after EMS application. Winged-bean seeds of both long and short pod types were soaked in wet paper towels for 6 hours followed by soaking in EMS of 0.3% and 0.5% for 4 hours. Winged-bean seeds of both pod types without EMS served as control. Ten of the 30 primers operon technologies USA and 2 primers bangalore genei showed sufficiently clear bands (OPA 1, OPA 3, OPA 10, OPA 15, OPA 19, OPB 1, OPB 6, OPB 7, ES10C27, and ES10C28). All the ten primers produced polymorphic fragments with a total number of 320 and size ranging from 100 bp to 2,000 bp. As many as 66 loci were observed. The polymorphism values obtained ranged from 16.67% to 46.97%. This low polymorphism values indicate that EMS concentrations applied have not resulted in sufficiently high genetic variation. At the same time, the genetic distance observed were only 0.11 to 0.41.
RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java Agus Hery Susanto; Ali Romadhoni; Murni Dwiati
Molekul Vol 17 No 2 (2022)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2022.17.2.6206

Abstract

Rhynchostylis gigantea (Lindl.) Ridl. is an orchid species spread over Southeast Asia countries. This species is very popular among ornamental plant collectors, especially due to its densely pack inflorescences. Hence, it is commercially found in many ornamental plant nursuries, such as Puspa Nirmala Orchids Banyumas, Central Java. Further development of the species should be supported by scientific data, particularly regarding the genetic variation. One of the molecular markers commonly used to study genetic variation is Random Amplified Polymorphic DNA (RAPD). This study aims to assess genetic variation of R. gigantea cultivars of Puspa Nirmala Orchids Banyumas collection by RAPD profiles. Genomic DNAs were extracted from leaf samples of eight R. gigantea individuals, while RAPD markers were amplified using five random primers (OPA-15, OPK-16, OPP-15, OPP-08 and OPO-08). Descriptive analysis was employed on the data obtained. It was revealed that all of the primers resulted in a 100% monomorphism. This indicates an extremely low genetic variation among R. gigantea population of Puspa Nirmala Orchids collection, which is probably due to the same origin from a selected hybrid of the same crosses.
Co-Authors Adi Amurwanto Ali Romadhoni Fajri Hartanti Murni Dwiati