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Des Roza
Balai Besar Riset Perikanan Budidaya Laut Gondol
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Peningkatan Respon Imun Non-Spesifik Benih Kerapu Bebek, Cromileptes altivelis dengan Imunostimulan dan Bakterin terhadap Infeksi Viral Nervous Necrosis (VNN) Des Roza; Fris Johnny; Tridjoko Tridjoko
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.160

Abstract

An experiment to evaluate the effectiveness immunostimulant and bacterin on humpback grouper have been conducted at the Disease Laboratory of Research Institute for Mariculture, Gondol, Bali. The experiment was designed in completely randomized design with four treatments in duplicates. Two hundred of humpback grouper juveniles (15-18 cm of total length, 55-65 g of body weight) were injected intraperitoneally with (A) bacterine at 107 cfu/kg body weight (BW), (B) peptidoglycan at 100 mg/kg BW, and (C) immuno star at 100 ml/kg BW, (D) control. The fish were then challenged with VNN by intramuscular injection at 10 days post treatment. Results showed that survival rates of juvenile after challenged with VNN were 60.00% (B & C), 53.34% (A), and 1.67% (control). Parameters of non-spesific immune respons showed that phagocytic activities were 17.56% (B), 17.55% (C), 13.11% (A), and 9.33% (control). In addition, lisozyme activities were 1.64 cm (B), 1.58 cm (C), 1.55 cm (A), and 1.46 cm (control). Immunostimulant and bacterin stimulated non-specific immune response, and increased survival rate of humpback grouper juvenile.
APLIKASI BAKTERIN SEBAGAI IMUNOSTIMULAN UNTUK PENCEGAHAN INFEKSI VIRAL NERVOUS NECROSIS (VNN) PADA BENIH IKAN KERAPU BEBEK, Cromileptes altivelis Des Roza; Fris Johnny
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8875

Abstract

An experiment on application of immunostimulant bacterin in order to prevent Viral Nervous Necrosis (VNN) in seed production of humpback grouper, Cromileptes altivelis has been conducted in Gondol Research Institute for Mariculture hatchery. The experiment arranged in completely randomized design with two replicates. One thousand and five hundred fry with total length 4 cm were treated as followed ; 1 ml bacterin/L sea water by immersion (Treatment A), 1 ml bacterin/kg pellet by oraly (Treatment B), and without bacterin as a control (C). Bacterin was immersion and oraly every 10 days for 60 days rearing period. Challenge test with VNN was carry out at day 30 and 60. At the end of experiment the non-spesific immune were examined. Result of challenge test performed at day 30 showed that treated fish gave positive response to bacterin treatment. The highest survival showed by treatment B (65.0%) followed by treatment A(50.0%) and C (22.5%). The same result also showed after challenge test at day 60. The highest survival was 72.5% (treatment B) followed by 67.5% (treatment A) and 35.0% (control). Phagocytic activity (PA) of treatment B was 15.5%, and 14.0% from treatment A compared to 8.0% in control. Lysozyme activity (LA) of treated groups were also higher than control namely 1.8 cm for treatment B followed by 1.6 cm for treatment A, and only 1.2 cm in control. Addition of bacterin immunostimulant in feed gives is survival rate higher compared to way of immersion and control. Statistically non-spesific immune response of humpback grouper was significant different among treatment (P<0,05).
PENINGKATAN IMUNITAS BENIH IKAN KERAPU LUMPUR, Epinephelus coioides TERHADAP INFEKSI VIRUS IRIDO DENGAN APLIKASI VITAMIN C DAN BAKTERIN Fris Johnny; Des Roza; Agus Priyono
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8881

Abstract

An  experiment  to  increase  of  immune  system  of  mangrove  grouper,  Epinephelus coioides seed with application of vitamin C and immunostimulant have been done. The experiment was carried out in completely randomized design with three treatments in twoplicates. The mangrove grouper seed (5 cm total length) were treated with 1000 mg vitamin C/kg diet (A), 1000 mg vitamin C + 1 ml bacterin/kg diet (B), and control (C). Vitamin C and bacterin were given every 10 day during 60 days rearing. After 30 and 60 days fishes were challenged with irridovirus. At the end of experiment, the non-specific immune responses were examined. The result showed that at challange test I, the highest survival rate was at treatment B (56.6%), followed by A (55.0%) and control (41.7%). On challenge test II, the higest survival rate was at treatment B (76.7%) followed by treatment A (68.4%) and control (48.3%).  The highest phagocytic activity (PA) was obtained at treatment B (21.0%), followed by treatment A (18.5%) and control (9.5%). The highest of lysozyme activity (LA) value was obtained on treatment B (2.0 cm), followed by treatment A (1.9 cm) and control (1.3 cm).  Combination of addition of ascorbic acid and immunostimulant gave higher survival rate compared to addition of immunostimulant only and control. Non-spesific immune response of mangrove grouper fry was significant different among treatment (P<0,05).
Co-Authors Agus Priyono Fris Johnny Tridjoko Tridjoko