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Hilal Anshary
Jurusan Perikanan, Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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INFEKSI DAN PATOLOGI PARASIT Actinocleidus sp. (MONOGENEA) PADA INSANG IKAN LELE DUMBO, Clarias gariepinus Hilal Anshary
Jurnal Perikanan Universitas Gadjah Mada Vol 12, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.808 KB) | DOI: 10.22146/jfs.2944

Abstract

The aims of this research were to study level of infection of the monogenean parasite Actinocleidus sp. and its pathological effects on the gills of Catfish ( Clarias gariepinus). This research was conducted in April to June 2008 at several Catfish culture localities in Makassar and Maros, South Sulawesi. The fish were chategorized in three different ages group, i.e. small (5 < x ≤ 10 cm), middle (10 < x ≤15 cm) and large (x ≥15 cm). The totals of 270 fish were examined with 9 times frequency of sampling conducted every week.Prevalence of Actinocleidus sp. infection at the three culture facilities was very high (100%). Mean intensity at small, middle and large fish cultured in Adhyaksa were 85.9, 93.2 and 55.2, respectively. In Arief Rate, the mean intensity of infection at small, middle and large fish were 17.5, 15.1 and 7.2, respectively, whereas in Maros the mean intensity of infection were 56.7, 64.7 and 40.9, respectively. There was a high signifi cant difference of mean intensity between small, middle and large fish (P < 0.01). Pathological effects of the parasite Actinocleidus sp. on gills showed some damages, i.e. distal and basal hyperfl asia, fusion and overstimulated of mucus production. The high prevalence and mean intensity of the parasite infection indicating the worse culture management system applying by the Catfish farmers as well as by the high stocking density of the fish. The lower mean intensity of infection seen in the larger fish might be indicating that the larger fish had already developed immune system than the small ones.
IDENTIFIKASI MOLEKULER DENGAN TEKNIK PCR-RFLP LARVA PARASIT Anisakis spp (Nematoda: Anisakidae) PADA IKAN TONGKOL (Auxis thazard) DAN KEMBUNG (Rastrelliger kanagurta) DARI PERAIRAN MAKASSAR Hilal Anshary
Jurnal Perikanan Universitas Gadjah Mada Vol 13, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.3064

Abstract

Beragam spesies ikan laut dikenal sebagai inang perantara dari berbagai spesies parasit, termasuk nematoda Anisakis yang merupakan agen penyebab Anisakiasis pada manusia. Anisakiasis dapat terjadi pada manusia setelah memakan ikan mentah atau setengah matang yang terinfeksi oleh Anisakis sp. Di Indonesia, khususnya di Sulawesi Selatan, informasi tentang Infeksi Anisakis ikan sering kali diabaikan dan tidak terdokumentasi dengan baik. Oleh karena itu, penelitian ini dimulai dengan mengumpulkan larva nematoda dari dua spesies ikan (Auxis thazard dan Rastrelliger kanagurta). Organ tubuh yang diperiksa meliputi rongga perut, organ internal dan jaringan otot. Larva nematoda yang diidentifikasi secara morfologis menunjukkan Anisakis spp kemudian dipisahkan untuk identifikasi secara molekuler lebih lanjut. Parasit difiksasi dalam etanol 70% dan dilanjutkan dengan PCR menggunakan primer ITS-1, 5.8S dan ITS-2 regions. Secara morfologi, Anisakis spp. dapat dibedakan dengan Anisakidae lain oleh adanya ventriculus yang jelas terlihat padaAnisakis spp. di bawah mikroskop stereo. Anisakis tipe I dan tipe II dibedakan oleh adanya mukron yang hanya terdapat pada Anisakistipe I dan tidak adanya di Anisakis tipe II. Spesies yang diidentifikasi menggunakan PCR-RFLP menunjukkan bahwa nematoda yang ditemukan pada ikan tongkol dan ikan kembung adalah Anisakis Typica.
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