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All Journal Pharmacy Peradaban Journal (Pharm. PJ): Jurnal Farmasi Universitas Peradaban
Pudjono Pudjono
Program Studi Farmasi, Fakultas Sains dan Teknologi, Universitas Peradaban
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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IDENTIFIKASI BAHAN TAMBAHAN PANGAN FORMALIN PADA BAKSO DAN TAHU YANG BEREDAR DI KECAMATAN SIRAMPOG M. Fikri Haikal; Baedi Mulyanto; Pudjono Pudjono
Jurnal Farmasi Universitas Peradaban Vol 2 No 1 (2022): Pharmacy Peradaban Journal (Pharm. PJ): Jurnal Farmasi Universitas Peradaban
Publisher : Prodi Farmasi Fakultas Sains dan Teknologi Universitas Peradaban

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Abstract

Peraturan Menteri Kesehatan Republik Indonesia Nomor 033 tahun 2012 tentang Bahan Tambahan Pangan mencantumkan bahwa formalin merupakan zat yang dilarang digunakan sebagai bahan tambahan pada pangan salah satunya bakso dan tahu. Penelitian bertujuan untuk mengidentifikasi dan mengetahui kadar bahan tambahan pangan formalin yang terkandung dalam bakso dan tahu yang beredar di Kecamatan Sirampog Kabupaten Brebes Provinsi Jawa Tengah. Penelitian menggunakan analisis kualitatif dengan pereaksi asam kromatrofat dan analisis kuantitatif dengan spektrofotometri UV-Vis. Analisis kualitatif dengan cara mencampurkan filtrat sampel dengan asam kromatrofat 0,5% dalam asam sulfat 60% lalu dipanaskan kemudian mengamati perubahan warna menjadi ungu (lembayung). Analisis kuantitatif dengan cara mengamati serapannya pada panjang gelombang maksimum 590,0 nm. Hasil penelitian kandungan bahan tambahan pangan formalin pada bakso dan tahu yang beredar di Kecamatan Sirampog Kabupaten Brebes Provinsi Jawa Tengah, dari 5 sampel bakso dan 5 sampel tahu yang diberi kode A, B, C, D, dan E menunjukkan 5 sampel bakso dan 3 sampel tahu mengandung bahan tambahan pangan formalin. Dari hasil pemeriksaan spektrofotometri UV-Vis kadar bahan tambahan pangan formalin yaitu untuk sampel bakso sampel A sebanyak 55,4 µg/gram, sampel B sebanyak 59,4 µg/gram, sampel C sebanyak 50,6 µg/gram, sampel D sebanyak 52,4 µg/gram, sampel E sebanyak 49 µg/gram dan sampel tahu sampel A sebanyak 50,4 µg/gram, sampel B sebanyak 55 µg/gram, sampel E sebanyak 58,2 µg/gram.
IDENTIFIKASI SILDENAFIL SITRAT PADA JAMU KUAT PRIA YANG BEREDAR DI WILAYAH BUMIAYU DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI Nur Alimul Hakim; Tunjung Winarno; Pudjono Pudjono
Jurnal Farmasi Universitas Peradaban Vol 2 No 1 (2022): Pharmacy Peradaban Journal (Pharm. PJ): Jurnal Farmasi Universitas Peradaban
Publisher : Prodi Farmasi Fakultas Sains dan Teknologi Universitas Peradaban

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Abstract

Penelitian ini bertujuan untuk mengidentifikasi sildenafil sitrat pada sediaan jamu kuat pria yang beredar di Wilayah Bumiayu. Analisis kualitatif menggunakan KLT, pelarutnya adalah metanol, fase geraknya adalah metanol: kloroform (4: 1) dan fase diamnya adalah pelat Silica Gel GF254. Pengamatan noda dengan lampu UV 254nm. Hasil uji kualitatif pada 7 sampel positif sildenafil sitrat. Analisis kuantitatif menggunakan HPLC, fasa diam berupa kolom C18 (150mm x 3.9mm, 5µm) dan fasa gerak berupa buffer fosfat pH 3, metanol, rasio asetonitril (50:30:20). Sildenafil sitrat standar dengan konsentrasi 0,1mg/mL dalam fase gerak. Larutkan sampel uji yang diekstraksi dalam labu 100 mL menggunakan fase gerak. Pengujian menggunakan HPLC pada panjang gelombang 290nm dengan tekanan pompa 1 mL/menit dan volume injeksi 20µL. Hasil uji kuantitatif dari 7 sampel dengan kode A,B,C,D,E,F,G per kapsul adalah (103,53 mg), (90,23 mg), (33,44 mg), (45,63 mg) , (104,78 mg), ( 64,64 mg) dan (84,22 mg).
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN JAMBU BIJI (Psidium guajava,L.,) DENGAN PENYARI ETANOL DAN KLOROFORM TERHADAP PERTUMBUHAN Staphylococcus aureus Liana Fijriati; Luthfi Hidayat Maulana; Pudjono Pudjono
Jurnal Farmasi Universitas Peradaban Vol 2 No 1 (2022): Pharmacy Peradaban Journal (Pharm. PJ): Jurnal Farmasi Universitas Peradaban
Publisher : Prodi Farmasi Fakultas Sains dan Teknologi Universitas Peradaban

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Abstract

The high mortality rate in the world, especially in tropical areas such as Indonesia, is one of the causes of infectious diseases. Infectious disease is one of the problems in the health sector that many Indonesian people suffer from which from time to time continues to grow. This study aims to determine the antibacterial activity of guava leaf extract with 70% ethanol and chloroform extract against Staphylococcus aureus based on the diameter of the inhibition zone. The concentrations of the extracts used were 5%, 10%, and 15%. For the positive control, amoxicillin was used, and the negative control was 10% DMSO. Extraction was carried out by maceration by soaking 250 grams of guava leaf powder in 500 ml of 70% ethanol. The filtrate from the maceration was filtered, then the residue was macerated again with 500 ml of chloroform filter, then evaporated in a rotary evaporator. Produces a thick extract in ethanol as much as 10 grams, and in chloroform as much as 6 grams. The extract obtained was then tested for its antibacterial activity using Nutrient agar media by well diffusion. The results obtained showed the presence of antibacterial activity in each filter, namely 5%, 10% and 15% ethanol extract, respectively, of 4.6 mm; 8mm and 10 mm. Meanwhile, in 5% , 10% and 15% chloroform, respectively, they were 4.3 mm; 7.6 mm and 10.3 mm. Positive control of 20 mm amoxicillin and negative control with 10% DMSO solvent did not show any antibacterial activity. From the results of the study, it was also found that the concentration of 15% in both extracts had the highest antibacterial activity in inhibiting the growth of Staphylococcus aureus. Keywords: Guava leaves, Staphylococcus aureus, antibacterial, inhibition, well diffusion.
Formulasi Dan Evaluasi Mutu Fisik Sediaan Body Scrub Cream Varietas Ubi Jalar Orange Dan Ubi Jalar Putih Dalam Fase Air Dan Minyak Syaekhoni Laelatul Latifah; Pudjono Pudjono; Resa Frafela Rosmi
Jurnal Farmasi Universitas Peradaban Vol 2 No 1 (2022): Pharmacy Peradaban Journal (Pharm. PJ): Jurnal Farmasi Universitas Peradaban
Publisher : Prodi Farmasi Fakultas Sains dan Teknologi Universitas Peradaban

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Abstract

Free radicals are one of the causes of premature aging due to oxidative stress in the body, such as rough, dull, and dry skin. This triggers the need for skin protection against free radicals. One of the natural ingredients that can be used is sweet potato (Ipomoea batatas L.). The purpose of this study was to determine the antioxidant activity of body scrub cream preparations of orange sweet potato and white sweet potato varieties and to determine variations in body scrub cream formulations that were effective as antioxidants. Detection of beta-carotene content by TLC using petroleum ether/benzene (9:1) eluent showed results that were in accordance with the comparison standard of beta-carotene in both orange and white sweet potatoes showing yellow spots with an Rf value of 0.8. The body scrub cream in this study was made with formulas F1, F2, and F3 for orange sweet potatoes and formulas F4, F5, and F6 for white sweet potatoes. The results showed that the F3 formula had greater spreadability, while the F3 and F6 formulas provided a longer adhesion time, and did not irritate the skin. The antioxidant activity test using the DPPH (1,1-diphenyl-2-Picrylhydrazil) method showed that the body scrub cream with the formula F1, F2, F3, F4, F5, and F6 had antioxidant activity with an IC50 value of 30,38 μL/mL; 19,37 μL/mL; 11,18 μL/mL; 73,91 μL/mL; 40,04 μL/mL; and 33,19 μL/mL. The orange sweet potato variety with a concentration of 50% and an IC50 value of 11,18 μL/mL was the best antioxidant.
Co-Authors Baedi Mulyanto Liana Fijriati Luthfi Hidayat Maulana M. Fikri Haikal Nur Alimul Hakim Resa Frafela Rosmi Syaekhoni Laelatul Latifah Tunjung Winarno