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Dwinita W Utami
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IDENTIFIKASI ALEL GEN Xa7 PADA PLASMA NUTFAH PADI LOKAL PAREKALIGOLARA MELALUI UJI SEGREGASI FENOTIPE DAN GENOTIPE [Identification of Xa7 Alleles Gene in Landrace Parekaligolara by Phenotype and Genotype Segregation Analysis] Utami, Dwinita W; Kadir, TS; Nasution, A
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.435 KB) | DOI: 10.14203/beritabiologi.v11i1.457

Abstract

Bacterial Leaves Blight (BLB) is one of the major diseases in rice. Among the disease resistance genes HDB, Xa7 gene still can compensate the diversity of Indonesian BLB pathogen in the field. One of the local rice germplasm, Parekaligolara (Indica, IRN: 1541) were selected based on allele mining research which contained a Xa7 alleles. The purpose of this study is to identify the alleles of the Xa7 gene contained in Indonesian local rice Parekaligolara germplasm in rice through the segregation testing based on phenotype and genotype performance of segregating population derived from the crossed with the susceptible control varieties, TN1. The segregation testing were conducted in F1 and F2 populations and analyzed on phenotypes of BLB resistance and genotypes using Xa7SNP markers. The analysis results showed that the Xa7 allele variation founded in the Parekaligolara germplasm are act as dominant alleles that contribute on the BLB resistance trait particularly to Race VIII of BLB pathogen. Xa7SNP8 is polymorphic markers for Xa7 alleles gene that are found in Parekaligolara. This marker could be used as a marker assisted for selection, if Parekaligolaras Xa7 alleles will use as a parent in the BLB disease resistant rice breeding program.
SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH Utami, Dwinita W; Ilhami, Aderahma; Hanarida, Ida
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (946.934 KB) | DOI: 10.14203/beritabiologi.v10i2.1966

Abstract

Red rice is one corp that has genetic variation both based upon phenotype and genotype evaluation using moleculer markers for specific gene. A research objective is to identify genetic diversity using red rice specific molecular markers. Forty accessions of Indonesian local rice included 9 accessions of red rice germplasm were used as genetic materials for this research. Specific molecular markers (RC3, RC9 and RC12) for a pigment color found in rice seed were designed based on alignment sequence analysis to rc-bHLH gene, a transcription factor for prothocyanidine protein. Then these markers were used to analyze the genetic variation of red rice. Cluster analysis and association test between phenotype and genotype performances were analyzed by TASSEL 2.1 software program. Results showed that red rice accessions distributed into 2 cluster. One cluster is more closely related to white rice than the other. The association test showed RC12 is the most significant marker in association with the red rice trait: red pericarp and white aleuron. These phenotype variations were found in Cempo merah, one accession originated from Yogyakarta and Pulut mandoti, one accession from South of Sulawesi.
Co-Authors IDA HANARIDA Ilhami, Aderahma Kadir, TS Nasution, A