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Waeniati Waeniati
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PERBANYAKAN TANAMAN MELON (CUCUMIS MELO L.) SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DAN BENZIL AMINO PURIN (BAP) Lidyawati, Ni Nyoman; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

The research on organogenesis of melon (Cucumis melo L.) was done at Tissue Culture Laboratory Forestry Faculty UNTAD Palu, during January until May 2012. The aim of this research was to determine the best concentration of IAA and BAP hormones in inducing organs of this plant.  Sterile seedling of melon was used as explants in this work. The experiment was arranged in  Completely Randomized Design (CRD)  with  4 treatments and 4 replications. The treatments were MS0 + 0,1 ppm IAA + 0,5 ppm BAP (M1), MS0 + 0,1 ppm IAA + 0,7 ppm BAP (M2), MS0 + 0,1 ppm IAA + 0,9 ppm BAP (M3) and MS0 + 0,1 ppm IAA + 1 ppm BAP (M4). Parameters observed on this study were the day appear of shoot and leaves, the number of shoots and leaves and also the present of callus and root. The result showed that the best medium for organs induction was MS0 + 0,1 ppm IAA + 1 ppm BAP, which shoot and leaf emerged in the day of 3,75 and 5,75 after induction, respectively. This media was also induced the number of shoots and leaves, i.e 4,75 and 8,75, respectively. Nevertheles this media was not suitable enough in inducing root formation.
PENGARUH PENAMBAHAN AIR KELAPA DAN BERBAGAI KONSENTRASI HORMON 2,4-D PADA MEDIUM MS DALAM MENGINDUKSI KALUS TANAMAN ANGGUR HIJAU (VITIS VINIFERA L.) Dwi PYD, Niluh Made; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Green grape (Vitis vinifera L.) is popular cultivar of grape that produce valuable of table fruit whic usually grown via vegetative propagation. Another  chalanging tecnology in grape propagate is by induction of callus in tissue culture tecnique. The study on grape callus induction was done at Tissue Culture Laboratory-Forestry Faculty-Tadulako University Palu, during periode of January 2012 until May 2012. This experiment was arranged in completely randomized design with 3 different mediums as treatments and 3 replications. The treatments were MS0 + 2,4-D 0.5 ppm + 10% coconut water (D1), MS0 + 2,4-D 1 ppm + 10% coconut water (D2), dan MS0 + 2,4-D 1.5 ppm + 10% coconut water (D3). The observed parameters in this research were the days appear of callus, percentage of explant producing callus (%), also observation on morphology and the cell of callus. The result showed that the MS medium with 10% coconut water and 2.4-D could induce the callus of the green grape and the best medium was MS0 + 2.4-D 1.5 ppm + 10% coconut water (D3). It was shown by responding in emerging of callus in 11 days after induction, the percentage of explant producing callus was 76.67%. Produced callus was active in cell proliferation,  and it has a compac texture, with brown-greeny color of callii.
PERTUMBUHAN ORGAN TANAMAN BUAH NAGA(HYLOCERUS UNDATUS) PADA MEDIUM MS DENGAN PENAMBAHAN BAP DAN SUKROSA Sulistiami, Ari; Waeniati, Waeniati; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Dragon fruit (Hylocyrus undatus) is a commercial-cultivated plant that has a high economic value and believed to be a healthy-food. Propagation of seedling by tissue culture technique is challenging step during cultivation of these plant. This research was aimed to determine the efeect of sucrose and BAP concentration in the dragon fruit (H. undatus). This experiment was arranged in completely randomized  design with 4 treatments and 3 replications. The treatmnts were : MS0 + 0,5 ppm BAP + 35 g/L sucrose (A1), MS0 + 0,5 ppm BAP + 405 g/L sucrose (A2), MS0 + 0,5 ppm BAP + 45 g/L sucrose (A3), and MS0 + 0,5 ppm BAP + 50 g/L sucrose (A4). The results showed that all medium tested were suitable medium in inducing organs, including emerge of buds, thorns and roots. The best medium in inducing organogenesis of the plant were MS0 + 0,5 ppm BAP + 50 g/L sucrose.
INDUKSI KALUS TANAMAN KAKAO (THEOBROMA CACAO L.) PADA MEDIA MS DENGAN PENAMBAHAN 2,4-D, BAP DAN AIR KELAPA Ariati, Sri Niken; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.743 KB)

Abstract

Study on callus induction of cacao (Theobroma cacao L.) was conducted at the Tissue Culture laboratory, Forestry faculty, Tadulako University, Palu, during periode of January until April 2012. This research was aimed to obtain the best medium formulation for induction and growth of cacao callus. Calli was induced from embryo  on MS based medium containing of 2,4-D, BAP and coconut water. The intact plant as explant was taken from Local Farming. This experiment was arranged in completely randomized  design with 4 treatments and 3 replications. The treatments were : MS medium + 2 ppm 2,4-D + 15% coconut water (N1), MS medium + 2 ppm 2,4-D + 0,2 ppm BAP, 15% coconut water (N2), MS medium + 2 ppm 2,4-D + 0,2 ppm BAP (N3), and MS medium + 2 ppm 2,4-D (N4). Parameters observed in this study were the days appear of callus, percentage of explant producing callus (%), also observation on morphology and the cell of callus. The best medium for callus  induction was MS medium + 2 ppm 2,4-D + 15% coconut water (N1). This medium produces healthy-compac callus which active in cell proliferation, in 6 days after induction.
Co-Authors Ali Djamhuri Ari Sulistiami I Nengah Suwastika Ni Nyoman Lidyawati Niluh Made Dwi PYD Sri Niken Ariati