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A.E.T.H. Wahyuni
Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna No. 2, Bulaksumur Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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VIRULANCE FACTOR OF Staphylococcus sp. ISOLATED FROM SUBCLINICAL MASTITIS IN ETTAWA GRADE GOAT’S MILK IN SLEMAN REGENCY -YOGYAKARTA Suwito, W.; Nugroho, W.S.; Wahyuni, A.E.T.H.; Sumiarto, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.52-57

Abstract

Stapphylococcus sp., is bacteria that caused subclinical mastitis in Ettawa Grade (EG) goat. Thepurpose of this study was to determine virulance factor Stapphylococcus sp., which was isolated fromsubclinical mastitis EG goat’s milk in Sleman regency, Yogyakarta. A total of 7 isolate Stapphylococcussp., were isolated from subclinical mastitis EG goat’s milk were determinated by several virulancefactors such as haemolysin, clumping factor, and coagulase. Haemolysin was determinated by culture inblood agar plate and incubated in the temperature of 37°C for 24 hours. Clumping factor wasdeterminated by mixing the rabbit plasma with Stapphylococcus sp., in the glass objects. Coagulase wasdeterminated by mixing the rabbit plasma and broth culture of Stapphylococcus sp. After incubated inthe temperature of 37°C for 24 hours in tube, then the gel formation was observed. Haemolytic type ßwas yielded from 5 isolate Stapphylococcus sp., whereas 2 isolates were not haemolytic. Clumpingfactor and coagulase were produced from 2 isolate Stapphylococcus sp. This study showed that not all ofStapphylococcus sp., isolate causing subclinical mastitis in EG goat have virulance factor.
THE SURFACE CHARACTER OF Staphylococcus aureus ISOLATED FROM SUBCLINICAL MASTITIS OF DAIRY COW SUPPORTING ADHERENCE TO UDDER EPITHELIAL CELL Wahyuni, A.E.T.H.; Winarso, D.; Valenti, V.; Franky, F.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 3 (2010): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.3.206-212

Abstract

Staphylococcus aureus (S. aureus) is one among pathogen bacteria that caused mastitis in dairycow in Indonesia. Its ability to initiate infection depends on the surface character of the bacteria. Thesurface character of S. aureus is determined by the corelation of red blood cell hemagglutination activity,hydrophobicity, production of protein A, and bacterial adherence to the mammary epithelial cells of thehost. This research was designed to characterize the surface of S.aureus, which correlates to bacterialadherence. Four isolates of S. aureus were taken from subclinical mastitis milk of dairy farm in Malang,East Java. The surface character of S.aureus was determined by performing the red blood cellhemagglutination test using the dairy cow erythrocyte suspension with concentration of 0.5%, 1%, and2%. The hydrophobicity expression was determined by Salt Aggregation Test (SAT) method using theAmmonium Sulfate suspension with concentration of 1.2 M, 1.6 M, 2.0 M, 2.4M, and 3.2 M. Serum softagar (SSA) test was used to indicate the production of protein A; Ability of adhesion was done byadhering the S.aureus, which were already labelled by Fluorescent Ichtiocyanate (FITC) to themammary epithelial cell of post-calving mice. Result of this this research showed that the surfacecharacters of S.aureus, which correlates to the adherence, comprise of erythrocytes hemagglutinationability, hydrophobicity, and protein A production. Isolates of S.aureus, which is hydrophobic, possesshaemagglutinine, and protein A were able to adhere to mammary epithelial cell with higher quantity
Co-Authors B. Sumiarto D. Winarso F. Franky V. Valenti W. Suwito W.S. Nugroho