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All Journal Animal Production : Indonesian Journal of Animal Production
R Wasito
Fakultas Kedokteran Hewan Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Follicular Development Dynamics and Plasma Progesterone Profile during the Estrous Cycle of Dairy Cows Putro, PP; Wasito, R; Wuryastuty, H; Indarjulianto, S
ANIMAL PRODUCTION Vol 10, No 2 (2008): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

A total of five local Friesian cows, 4 to 5 years, healthy, reproductively sound, were used in the study to understand the development dynamics of dominant follicles and plasma progesterone profile during estrous cycle.   Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz transducer was performed daily for one full cycle to follow the development of dominant follicle and corpus luteum dynamics.   Blood plasma was collected daily to determine progesterone levels using EIA technique.   Follicular development dynamics and plasma progesterone levels were analyzed statistically using analyses of variance, while correlation between plasma progesterone levels and corpus luteum size were tested using correlation analyses.   The length of estrous cycle in local Friesian cow was 21.00 + 1.00 days.   Follicular dynamics during estrous cycle indicated only had two waves of dominant follicular development.   The first wave of dominant follicle was firstly identified on day 3, reached maximum diameter (11.17 + 0.90 mm) on day 11 and identifiable until day 15.   The second dominant follicle appeared on day 11, reached maximum size on day 21 (13.17 + 0.69 mm) and underwent ovulation on the next day.   The increased diameter of dominant follicle from days 17 to 21 was linear with growth rate of 1.20 + 0.18 mm/day.   Ovulatory dominant follicle had larger size than non-ovulatory ones (P<0.05).   Corpus luteum was ultrasonically detectable from days 5 to 19 of the estrous cycle.   The maximum size of corpus luteum (11.83 + 0.75 mm) attained on day 11, and then regressed substantially till day 19.  Plasma progesterone level started to rise during the luteal phase, reached the peak level on day 12 (4.64 + 0.23 ng/ml) and then decreased steadily till the lowest level (0.42 + 0.05 ng/ml) at the time of estrus.  The plasma progesterone profile and the corpus luteum development were concomitant each other during estrous cycle of the cow.  It can be concluded that the follicular dynamics during estrous cycle in local Friesian cows only had two waves of dominant follicular development, while plasma progesterone levels manifested corpus luteum function throughout the estrous cycle. (Animal Production 10(2): 73-77 (2008) Key Words:  Follicle, corpus luteum, progesterone, estrous cycle, cow
Identification of Excretory – Secretory Sporozoite Antigens of Eimeria tenella with Monoclonal Antibody Prastowo, J; Nurcahyo, W; Kurniasih, Kurniasih; Wasito, R
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research done specifically to identified the. Eimeria tenella excretory-secretory sporozoit antigen by using the monoclonal antibody reaction instead. Eimeria tenella excretory-secretory sporozoit antigen. The 107 sporozoits were obtained from existation of 5x107 oocysts with 25 ml tripsin and sodium taurocholat. Excretion - secretion antigen were preparated by freezing and thawing and then be isolated with ice bath 20 second, 20 meter of amplitude as the further treatment.  Determinating the excretion -secretion sporozoit protein by using BCA-test. While the visualization sporozoit protein antigen by SDS-PAGE. Monoclonal antibody were produced through hybridization between B BALB/c limphosit immunized with Eimeria tenella excretion-secretion sporozoite antigen and myeloma cells. The monoclonal antibody identification  of excretion – secretion antigen were done through western blott. The visualization of protein molecule weight of Eimeria tenella sporozoit were resulted 20 protein fraction, included 14, 16, 17, 18, 20, 24, 26, 32, 34, 36, 39, 43, 47, 56, 65, 67, 76, 87, 94, and 96 KDa. There were 12 kinds of monoclonal antibody could be produced from these proteins. While the 5 hybridoma immunoblotting resulted specific reaction by the appearances of reaction ribbon obviously, there were MABset 1 which identified protein epitop with 14.7 KDa of molecule weight,  MAbset 2 which identified protein epitop with 43 KDa of molecule weight, MAbset 3 which identified protein epitop with 42 KDa of molecule weight, MAbset 4 which identified protein epitop with 47 KDa of molecule weight and MAbset 5 which identified protein epitop with 90 KDa of molecule weight. As the conclusion, five of excretion-secretion sporozoit antigen were proper immunogen to stimulate the hospest immunity. (Animal Production 7(2): 95-100 (2005) Key Words: Sporozoit, Antigent, Eimeria tenella, Coccidiosis
Co-Authors H Wuryastuty, H J Prastowo, J Kurniasih Kurniasih PP Putro, PP Soedarmanto Indarjulianto W Nurcahyo, W