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All Journal Indonesian Journal of Biotechnology Sciscitatio : Journal for Biological Science
Barinta Widaryanti
Cancer Chemoprevention research center, Faculty of Pharmacy, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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PGV-1 is a Potent Antimitotic Agent Widaryanti, Barinta; Da’i, Muhammad; Kawaichi, Masashi
Indonesian Journal of Biotechnology Vol 13, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.837 KB)

Abstract

Carcinogenesis may resulted from the malfunctioning of programmed cell death. Most of the anticancer drugs incurrent use induce apoptosis in susceptible cells. The fact that disparate agent interacting with different targets seemto induce cell death through some common mechanisms suggest that anticancer activity is determined by the abilityof inhibiting cell growth. Pentagamavunon-1 (PGV-1) is one of the curcumin analogues which showed to havepotency in inhibiting proliferation of T47D human breast carcinoma cells. The effects on T47D cells growth isassociated with cell cycle arrest in G2/M phase at the concentration of 2.5 ?M, followed by hyperploidy. The data onpolymerization assay, indicated that PGV-1 interact with tubulin in different manner from taxol. PGV-1 inhibittubulin polymerization on cell culture while taxol stabilized tubulin polymerization. Immunostainning data onPGV-1 treated cells showed slightly tubulin condensation, while taxol treated cells showed tubulin condensationdistinctly at 12 minutes after releasing from depolymerizing agent.In conclusion, PGV-1 represent a new microtubule inhibitor and has the potential to be developed for antimitoticdrugKey words: Pentagamavunon-1, T47D, tubulin
Seleksi Bakteri dengan Aktivitas Fibrinolitik yang Diisolasi dari Tanah Rumah Potong Ayam Khikmah, Nur; Astrika, Nuri Rizky; Widaryanti, Barinta
SCISCITATIO Vol. 5 No. 1 (2024): Volume 5 Number 1, January 2024
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.51.159

Abstract

Bakteri fibrinolitik mampu melisiskan fibrin, suatu bekuan darah yang terbentuk dari fibrinogen melalui proses penghancuran protein oleh trombin. Akumulasi fibrin yang berlebihan tanpa keseimbangan hemostatis menyebabkan trombosis, dapat mengakibatkan kelainan serebrovaskular dan kardiovaskular. Enzim fibrinolitik dari bakteri mempunyai potensi sebagai agen obat trombosis. Tujuan penelitian ini mendapatkan bakteri yang unggul dalam menghasilkan enzim fibrinolitik. Isolat bakteri diseleksi berdasarkan Indeks Aktivitas Enzim (IAE) proteolitik pada skim milk agar dan IAE fibrinolitik pada fibrin plate agar. IAE diperoleh dengan membagi diameter zona jernih di sekeliling koloni bakteri dengan diameter koloni bakteri. Dua puluh delapan isolat bakteri mempunyai aktivitas proteolitik , empat belas isolat diantaranya mempunyai aktivitas fibrinolitik. Lima isolat bakteri mempunyai IAE fibrinolitik 4,0. Isolat tersebut teridentifikasi sebagai anggota Bacillus
Co-Authors Astrika, Nuri Rizky Da'i, Muhammad Masashi Kawaichi, Masashi Nur Khikmah, Nur