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All Journal Jurnal Pembelajaran dan Biologi Nukleus
Fahimatul Ulya
Institut Teknologi dan Sains Nahdlatul ulama pasuruan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Isolation and Selection of Sulfuric Acid Bacteria from Kejayan River as Bio-oxidation Agents Ulya, Fahimatul
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2: Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2928

Abstract

Biooxidation method is connsidered more environmentally friendly and more economical to be able to overcome environmental problems polluted by metals. Sulfuric acid-producing bacteria is one of the biooxidizing agents, these bacteria can be isolated from acidic soil or acidic areas. The Kejayan Pasuruan River is drained by various industrial acidic wastes that contain metals. This study aims to obtain sulfuric acid-producing bacteria from the Kejayan river. Isolation and selection of sulfuric acid-producing bacteria from Kejayan river soil using thiosulfate medium. Pure isolates were obtained using the streak plate quadrant. In the biooxidation process using CuFeS2, sulfuric acid levels were measured using the turbidimetric method, the levels of [Cu] and [Fe] in the biooxidation medium were tested using AAS (Atomic Absorption Spectroscopy). K.2 isolate was selected as sulfuric acid producing bacteria with the highest sulfate concentration [SO42-] 13.9 ppm at 48 hours. These bacteria have the potential as biooxidizing agents for Cu and Fe metals.
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