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Karakter Fisiologis dan Peranan Antibiosis Bakteri Perakaran Graminae terhadap Fusarium dan Pemacu Pertumbuhan Tanaman Pisang Munif, A; Djatnika, I; Widodo, -
Jurnal Hortikultura Vol 17, No 2 (2007): Juni 2007
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK. Perakaran tanaman famili Graminae memiliki kerapatan populasi bakteri yang tinggi dan berpotensi digunakan sebagai agensia pengendalian hayati. Penelitian ini bertujuan mempelajari karakter fisiologis bakteri rizosfer dan endofit perakaran tanaman famili Graminae serta peranannya sebagai bakteri antibiosis terhadap Fusarium oxysporum f. sp. cubense (Foc) secara in vitro dan pemacu pertumbuhan tanaman. Tanaman indikator yang digunakan dalam uji kemampuan bakteri dalam memacu pertumbuhan tanaman adalah mentimun. Penelitian dilaksanakan di Laboratorium Mikologi dan Rumah Kaca Institut Pertanian Bogor, berlangsung dari bulan Juni sampai November 2003. Hasil penelitian menunjukkan bahwa perakaran rumput gajah memiliki kerapatan populasi bakteri rizosfer yang paling tinggi yaitu 8,12 log cfu/g bobot basah akar, sedangkan populasi bakteri endofit yang tertinggi ditemukan pada perakaran sorgum yaitu 4,02 log cfu/g bobot basah akar. Empat puluh dua isolat dari 182 isolat diuji karakter fisiologisnya menggunakan medium spesifik. Semua isolat yang diuji, masing-masing sebesar 60,41, 52,08, 4,16, 18,75, 52,09, dan 18,75%, memiliki kemampuan aktivitas selulolitik, proteolitik, kitinolitik, produksi HCN, mela-rutkan fosfat, dan fluoresensi. Berdasarkan hasil uji secara in vitro menggunakan rancangan acak lengkap, diketahui bahwa 21,45% dari isolat bakteri yang diuji, memiliki kemampuan antibiosis terhadap Foc, dan lebih sepertiganya (7,70%) berasal dari bakteri endofit perakaran padi. Persentase daya hambat bakteri pada media TSA lebih tinggi dibandingkan pada media PDA dan PDA+TSA. Dari hasil uji secara in vivo pada tanaman mentimun menggunakan rancangan acak kelompok, diketahui bahwa 22,92% dari 52 isolat bakteri yang diuji, dapat meningkatkan secara nyata pertumbuhan tanaman. Hasil penelitian ini memberikan informasi bahwa bakteri yang berasal dari rizosfer dan endofit perakaran Graminae mempunyai kemampuan menghambat perkembangan F. oxysporum f.sp. cubense secara in vitro dan meningkatkan pertumbuhan tanaman.ABSTRACT. Eliza, A. Munif, I Djatnika, and Widodo. 2007. Physiological Characters and Antibiosis Activity of Gramineous Crops Rhizobacteria, Againts Fusarium and Banana Growth Promoting. Roots of Gramineous colonized by various populated bacteria are potential as biological control agents to various soil-borne plant pathogens. This research was conducted to study some physiological characteristics, antibiosis activity to Fusarium oxysporum f. sp. cubense (Foc), and plant growth promoting effect of gramineous crops roots bacteria to bananas. Cucumber was used as an indicator plant to detect plant growth activity. The experiment was conducted at Mycology Laboratory and Glasshouse Bogor Agriculture Institute from June until November 2003. Highest level population density of rhizobacteria (8.12 log cfu/g fresh root) was showed by giant grass roots, while endophytic bacteria were indicated by sorghum roots (4.02 log cfu/g fresh root). Forty two isolates of 182 isolates was physiologically characterized using specific medium. Among of tested bacteria, 60.41, 52.08, 4.16, 18.75, 52.09, and 18.75%, showed the capability in cellulolitic, proteolitic, HCN production, phosphate solubilization, and fluorescent activities, respectively. Using dual culture test of a randomized complete design, 21.45% of 182 isolates showed antibiosis activity against Foc, and more than one third (7.7%) of those were endophytic bacteria from rice roots. Inhibition ability of bacteria on TSA medium was higher than on PDA or PDA + TSA. Based on bioassay test in greenhouse used randomized block design, 22.92 % of 52 isolates have growth promoting activity on tested plant. Bacteria from rhizosphere and endophytic of Gramineous roots have ability to inhibit of F. oxysporum f. sp. cubense and increasing plant growth.
LELAGON DOLANAN ANAK DAN PENDIDIKAN KARAKTER Widodo, -
Harmonia: Journal of Arts Research and Education Vol 10, No 2 (2010)
Publisher : Department of Drama, Dance, and Musik (Sendratasik), Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/harmonia.v10i2.62

Abstract

Children folksongs laras slendro and pelog are full of noble values of national cultures. Nowadays, they are abandoned. Children as the subjects and appreciators of the songs get more unfamiliar with the songs. The songs are less often heard in the family, schools, playground and the place of living environments and other places of gathering for children. The lack of new creation of folksongs worsens the condition. New creation of the songs is badly needed to introduce, preserve, and plant the noble values of national cultures. When the songs and their values can get to more and more children, the character development of the children will be colored with the values of national cultures. They may grow and become generations that respect values of national cultures. Kata kunci: lelagon dolanan anak, pendidikan karakter, pelog dan slendro
NILAI-NILAI LUHUR DALAM LELAGON DOLANAN Widodo, -
Harmonia: Journal of Arts Research and Education Vol 9, No 2 (2009)
Publisher : Department of Drama, Dance, and Musik (Sendratasik), Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/harmonia.v9i2.646

Abstract

Lelagon dolanan (children) contains a variety of high values of national culturewhich is useful for building character and identity of the nation. Dewasa inikondisi lelagon dolanan (anak) ibarat hidup segan mati tak mau. He wassurrounded by a variety of industrial art products that appear more glamorous,practical, and economical. To preserve, develop, and distribute it needs to supportfrom various parties both actors musical arts, government, media entrepreneurs,and society. Javanese gamelan music art perpetrators should be creative,innovative, creative, and productive working lelagon dolanan (children) whoactually follow the new development era. The government is expected to makepolicy, especially through formal education institutions in favor of thepreservation and development of lelagon dolanan. Employers can participate in themass media through the production and dissemination of copyrighted workspublicly lelagon dolanan. Thus, the public can access easily and cheaply lelagondolanan products (children).Kata kunci: lelagon dolanan, nilai, luhur, pelestarian, pengembangan
Model Peningkatan Kinerja Pemasaran dalam Konteks Adaptabilitas Lingkungan Arif, Ahmad Lukman; Widodo, -
Jurnal Analisis Manajemen Vol 5, No 1 (2011): Jurnal Analisis Manajemen
Publisher : Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2600.625 KB)

Abstract

This study examines the performance improvement of marketing in the context of environmental adaptability, which examined the effect of creativity programs entrepreneurial orientation and marketing performance and creativity to the performance of marketing programs both before and in the context of environmental adaptability. The respondents are the managers of SMEs who are members of the Organization Centra Industries (OCI) Sculpture and Carving in Center Industry Mulyoharjo Jepara. which amounts to 157. Given the limited number of population, sampling method with the census method, meaning the number of samples equal to the population of which amounted to 157. The study results indicate that 1). When oriented entrepreneurial SMEs, the marketing of higher performance. This means that performance improvements constructed by the orientation of entrepreneurial marketing with innovation indicators, dare to take risks and act proactively 2). If SMEs have the creativity of marketing programs, the higher the marketing performance. This means that marketing performance improvement constructed by the creativity of marketing programs with indicators of reference, the discussion of marketing programs and compliance programs. 3). If SMEs have entrepreneurial orientation, the higher the creativity of marketing programs. It means increased creativity of marketing programs built by entrepreneurial orientation to the indicators of innovation, daring to take risks and act proactively 4). Moderating environmental adaptability and creativity of entrepreneurial orientation on performance marketing programs pemasaran.Artinya high environmental adaptability will increase the influence of entrepreneurial orientation and creativity of the marketing program marketing performance
LELAGON DOLANAN ANAK DAN PENDIDIKAN KARAKTER Widodo, -
Harmonia: Journal of Arts Research and Education Vol 10, No 2 (2010)
Publisher : Department of Drama, Dance and Music, FBS, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/harmonia.v10i2.62

Abstract

Children folksongs laras slendro and pelog are full of noble values of national cultures. Nowadays, they are abandoned. Children as the subjects and appreciators of the songs get more unfamiliar with the songs. The songs are less often heard in the family, schools, playground and the place of living environments and other places of gathering for children. The lack of new creation of folksongs worsens the condition. New creation of the songs is badly needed to introduce, preserve, and plant the noble values of national cultures. When the songs and their values can get to more and more children, the character development of the children will be colored with the values of national cultures. They may grow and become generations that respect values of national cultures. Kata kunci: lelagon dolanan anak, pendidikan karakter, pelog dan slendro
NILAI-NILAI LUHUR DALAM LELAGON DOLANAN Widodo, -
Harmonia: Journal of Arts Research and Education Vol 9, No 2 (2009)
Publisher : Department of Drama, Dance and Music, FBS, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/harmonia.v9i2.646

Abstract

Lelagon dolanan (children) contains a variety of high values of national culturewhich is useful for building character and identity of the nation. Dewasa inikondisi lelagon dolanan (anak) ibarat hidup segan mati tak mau. He wassurrounded by a variety of industrial art products that appear more glamorous,practical, and economical. To preserve, develop, and distribute it needs to supportfrom various parties both actors musical arts, government, media entrepreneurs,and society. Javanese gamelan music art perpetrators should be creative,innovative, creative, and productive working lelagon dolanan (children) whoactually follow the new development era. The government is expected to makepolicy, especially through formal education institutions in favor of thepreservation and development of lelagon dolanan. Employers can participate in themass media through the production and dissemination of copyrighted workspublicly lelagon dolanan. Thus, the public can access easily and cheaply lelagondolanan products (children).Kata kunci: lelagon dolanan, nilai, luhur, pelestarian, pengembangan
Karakter Fisiologis dan Peranan Antibiosis Bakteri Perakaran Graminae terhadap Fusarium dan Pemacu Pertumbuhan Tanaman Pisang Munif, A; Djatnika, I; Widodo, -
Jurnal Hortikultura Vol 17, No 2 (2007): Juni 2007
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v17n2.2007.p%p

Abstract

ABSTRAK. Perakaran tanaman famili Graminae memiliki kerapatan populasi bakteri yang tinggi dan berpotensi digunakan sebagai agensia pengendalian hayati. Penelitian ini bertujuan mempelajari karakter fisiologis bakteri rizosfer dan endofit perakaran tanaman famili Graminae serta peranannya sebagai bakteri antibiosis terhadap Fusarium oxysporum f. sp. cubense (Foc) secara in vitro dan pemacu pertumbuhan tanaman. Tanaman indikator yang digunakan dalam uji kemampuan bakteri dalam memacu pertumbuhan tanaman adalah mentimun. Penelitian dilaksanakan di Laboratorium Mikologi dan Rumah Kaca Institut Pertanian Bogor, berlangsung dari bulan Juni sampai November 2003. Hasil penelitian menunjukkan bahwa perakaran rumput gajah memiliki kerapatan populasi bakteri rizosfer yang paling tinggi yaitu 8,12 log cfu/g bobot basah akar, sedangkan populasi bakteri endofit yang tertinggi ditemukan pada perakaran sorgum yaitu 4,02 log cfu/g bobot basah akar. Empat puluh dua isolat dari 182 isolat diuji karakter fisiologisnya menggunakan medium spesifik. Semua isolat yang diuji, masing-masing sebesar 60,41, 52,08, 4,16, 18,75, 52,09, dan 18,75%, memiliki kemampuan aktivitas selulolitik, proteolitik, kitinolitik, produksi HCN, mela-rutkan fosfat, dan fluoresensi. Berdasarkan hasil uji secara in vitro menggunakan rancangan acak lengkap, diketahui bahwa 21,45% dari isolat bakteri yang diuji, memiliki kemampuan antibiosis terhadap Foc, dan lebih sepertiganya (7,70%) berasal dari bakteri endofit perakaran padi. Persentase daya hambat bakteri pada media TSA lebih tinggi dibandingkan pada media PDA dan PDA+TSA. Dari hasil uji secara in vivo pada tanaman mentimun menggunakan rancangan acak kelompok, diketahui bahwa 22,92% dari 52 isolat bakteri yang diuji, dapat meningkatkan secara nyata pertumbuhan tanaman. Hasil penelitian ini memberikan informasi bahwa bakteri yang berasal dari rizosfer dan endofit perakaran Graminae mempunyai kemampuan menghambat perkembangan F. oxysporum f.sp. cubense secara in vitro dan meningkatkan pertumbuhan tanaman.ABSTRACT. Eliza, A. Munif, I Djatnika, and Widodo. 2007. Physiological Characters and Antibiosis Activity of Gramineous Crops Rhizobacteria, Againts Fusarium and Banana Growth Promoting. Roots of Gramineous colonized by various populated bacteria are potential as biological control agents to various soil-borne plant pathogens. This research was conducted to study some physiological characteristics, antibiosis activity to Fusarium oxysporum f. sp. cubense (Foc), and plant growth promoting effect of gramineous crops roots bacteria to bananas. Cucumber was used as an indicator plant to detect plant growth activity. The experiment was conducted at Mycology Laboratory and Glasshouse Bogor Agriculture Institute from June until November 2003. Highest level population density of rhizobacteria (8.12 log cfu/g fresh root) was showed by giant grass roots, while endophytic bacteria were indicated by sorghum roots (4.02 log cfu/g fresh root). Forty two isolates of 182 isolates was physiologically characterized using specific medium. Among of tested bacteria, 60.41, 52.08, 4.16, 18.75, 52.09, and 18.75%, showed the capability in cellulolitic, proteolitic, HCN production, phosphate solubilization, and fluorescent activities, respectively. Using dual culture test of a randomized complete design, 21.45% of 182 isolates showed antibiosis activity against Foc, and more than one third (7.7%) of those were endophytic bacteria from rice roots. Inhibition ability of bacteria on TSA medium was higher than on PDA or PDA + TSA. Based on bioassay test in greenhouse used randomized block design, 22.92 % of 52 isolates have growth promoting activity on tested plant. Bacteria from rhizosphere and endophytic of Gramineous roots have ability to inhibit of F. oxysporum f. sp. cubense and increasing plant growth.