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IGM Wijaya P
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Journal : E-Jurnal Medika Udayana

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SEROTYPE IDENTIFICATION OF VIBRIO CHOLERAE ISOLATED FROM ICE THAT USE FOR MARINE PRODUCT PRESERVATIVE BETWEEN MODERN AND TRADITIONAL MARKET IN DENPASAR I P Ananta WS; IGM Wijaya P; IGP Dhinarananta; P Yuniadi A; M Agus Hendrayana
E-Jurnal Medika Udayana vol 2 no6(2013):e-jurnal medika udayana
Publisher : Universitas Udayana

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Abstract

Colera is a disease that cause by Vibrio cholerae (V. cholerae). V. cholerae ) is one of pathogen bacteria that can be gained from contaminated dietary sources. However, Indonesian people having indulgence in consuming seafood. They usually acquire the marine products at the nearby market. In the other side, unhygienic process in provision of raw materials possibly increase the contamination of V. cholerae. The purpose of this study are to obtain the contamination of V. cholerae on the marine products in Denpasar. This study using observational descriptive method with quotas sampling by taking fish ice preservative used by fish merchants in the modern market and traditional markets as the samples. Six samples taken in each location (traditional and modern market). Sample then cultured with medium alkaline peptone water ( APW ) continued with thiosulfate citrate bile salt sucrose ( TCBS ). The result of the TCBS culture then continued by undergo grams staining and latex serotyping procedure to identify type V. vholerae. Every samples then noted and compared. The result on samples of modern market found 5 from 6 samples ( 83,33 % ) positive contains V. cholerae with Inaba serotype. Samples of traditional market found 4 from 6 sample (66,67%) positive contains V. cholerae with inaba serotype and 1 of them (16,67%) with Hikojima serotype. This study represent that contamination of V. cholerae are still high and potentially endanger people of Denpasar.  
DETECTION OF Vibrio choleraeO1 SEROTYPE IN ICE PRESERVATIVES OF SEA PRODUCTS AT KEDONGANAN FISH MARKET, KUTA, BALI IGM Wijaya P; P Yuniadi A; IP Ananta WS; IGP Dhinarananta; M Agus Hendrayana
E-Jurnal Medika Udayana vol 2 no 9 (2013):e-jurnal medika udayana
Publisher : Universitas Udayana

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Abstract

Vibrio cholerae is one of gut attacking bacteria in which 20% of the victims suffered acute diarrhea and 10-20% of them were severe, in addition the mode of case in Indonesia are increasing. Bali is a threatened area especially by ice preservative of sea products investement since it has a tropical climate with tons of sea products, and one of most popular fish market is Kedonganan Fish Market. We were conducting a descriptive observational with explorative study which is aimed to detect V. cholerae investation in ice preservative. The samples are ice or melted ice which are used to preserve sea product at Kedonganan Fish Market and each were choosed randomly. The samples processed in Alkaline Peptone Water (APW) and planted on TCBS media for microbiological culture exploration which refer to Kobe University protocol of V. cholerae isolation from environment; painted for gram evaluation based on our departement’s procedures; and latex serology evaluation using tools of V. cholerae O1 AD ‘seiken’. Of 10 samples obtained, 21 single bacterial colonies were found, in which 8 of them were suspected as V. cholerae’s colonies based on microbiological culture and gram painting evaluation. The suspected colonies are then undergone latex serology test to make sure the existence of V. cholerae and knowing its serotype. Based on the result analysis and interpretation of microbiological culture test, gram painting, and serology exploration, its found that 50% of samples were invested by Inaba type of V. cholerae O1.
EROTYPE IDENTIFICATION OF VIBRIO CHOLERAE BACTERIAWHICH ISOLATED FROM ICE AMONGTUBE AND CUBE ICE TYPE IN FOOD AND BEVERAGES SELLER AT DENPASAR CITY, BALI IGP Dhinarananta; IGM Wijaya P; P Ananta WS; P Yuniadi A; M Agus Hendrayana
E-Jurnal Medika Udayana vol 3 no 1 (2014):e-jurnal medika udayana
Publisher : Universitas Udayana

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Abstract

Cholera is a type of watery diarrhea with specific sign stool containing mucus which resembles rice water. Cholera caused by gram negative bacteria Vibrio cholerae (V.Cholerae). The transmissions of bacteria were through a contaminated food or water.Bali is an international tourism destination with tropical weather where ice is widelyused in food and beverage which bring a risk of cholera through a contaminated ice.Iceshave a risk of bacterial contamination whether from the making and the usage process.Type of ice that widely used were cube and tube ice which each of them have a differentin making and usage process. The purpose of this study is to obtain the contamination ofV.cholera in cube and tube ice. The method of this study is descriptive observationalstudy with quota sampling technique. Sample were obtained from a restaurants andstreet vendor which use a block and tube ice with total 10 sample and 5 for each type ofice.Sample then cultured in Alkaline Peptone Water(APW) and Thiosulfate Citrate Bilesalt Sucrose(TCBS) agar. Bacteriacolony then identified using a gram staining andLatex Serotyping. The result are 3 over 5 (60%) sample of cube ice contaminated byV.cholera O1 Inaba serotype and 3 over 5 (60%) sample of tube ice contaminated byV.cholera O1 Inaba serotype.
Co-Authors I P Ananta WS IGP Dhinarananta IP Ananta WS Made Agus Hendrayana P Ananta WS P Yuniadi A