Wiwik Siti Windrati
Department of Product Agricultural Technology, Faculty of Agricultural Technology, University of Jember, Jember 68121

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effect of ascorbic acid on gel formation of myofibril from big eye scad fish (Selar crumenophthalnus) Achmad Subagio; Wiwik siti Windrati; Mukhamad Fauzi; Yuli Witono
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 2 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Effect of ascorbic acid on gel formation of myofibril from big eye scad fish (Selar crumenophthalnus)were studied for its development as food ingredient. Myofibril was galled by the addition of various concentrations of ascorbic acid (0, 0.1, 0.2, 0.3 and 0.4%) and the gels were then characterized for its cooking loss, of the gel, but at 0.4% the cooking loss of gel increased significantly. Accordingly, the WHC of the gel changed insignificantly with the ascorbic acid addition below 0.3%, and decrease sharply in the addition of 0.4%. Gel textures were affected by the addition of ascorbic acid at all levels, namely 29.9 ± 1.9, 31.0 ± 0.3, 35.4 ± 0.4, 46.7 ± 1.5, and 115.7 ± 3.2 g/7 mm for 0, 0.1, 0.2, 0.3 and 0.4%, respectively. Sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) showed that addition of ascorbic acid drove formation odfdisulphide bond in the myosin heavy chain (MHC) and other myofirillar proteins, resulting in the development of a strong three dimensions structure I myofibril gel as shown by microscopic structure. Key words : Ascorbic acid, Big eye Scad Fish (Selar crumenophthalnus), el formation myofibril
Characterization of Myofibrillar Protein from Goldband Goat Fish (Upeneus moluccensis) and Bigeye Scad Fish (Selar crumenophthalmus) Achmad Subagio; Wiwik Siti Windrati; Mukhammad Fauzi; Yuli Witono
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Characteristics of myofibrillar protein from goldband goat fish (U. moluccensis) and bigeye scad fish (S. crumenophthalmus) were studied for their development as food ingredient. Color analysis using chromameter showed that myfibrillar protein from goldband goat fish was light colored, while that of bigeye scad was slightly drak colored. Proximate analysis showed that their contents were similar by crude protein 7-10%, crude fat 0.2-0.5%, and ash 0.4-0.7%. Amino acid compositions of both myofibrillar proteins were very close, dominated by glutamic acid (20%), aspartic acid (10%) and lysine (9%). However, comparing with bigeye scad, myofibrillar proteins from goldband goatfish were easily aggregated, had higher gelation capacity and higher emulsion activity, but lower solubility. Based on these result, myofibrillar protein from goldband goatfish has good charachteristics as food ingredient especially for restructured products comparing with bigeye scad. Key words : Myfibrillar, goldband, goat fish, bigeye scad fish, proteins.
Effects of Addition of Protein Isolates from Jack Bean Seed (Canavalia ensiformis L.) on the Characteristics of Cake Achmad Subagio; Wiwik Siti Windrati; Yuli Witono
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Addition of jack bean (Canavalia ensiformis L.) protein isolate in cake making was studied. The isolate was prepared from the beans by the method of isoelectrical point at pH 4. At low concentrations (less than 1% of the wheat flour), the addition of the protein isolate could improve the characteristics of the cake by increasing the loaf volume, decreasing the density, and softening the texture. In contrast, the addition of 1.5% tended to decrease the quality of the cake as compared to that added with 1% by decreasing the loaf volume, increasing the density, and hardening the texture. However, the more protein isolate added, the more slowly the rate of the cake staling. Key words : Cake, jack bean, protein isolate.
KARAKTERISASI MIE MOJANG (MOCAF-JAGUNG) DENGAN PERBEDAAN JENIS DAN KONSENTRASI BAHAN PENGIKAT nurud diniyah; Denik Setiawati; Wiwik Siti Windrati; Achmad Subagio
Jurnal Penelitian Pascapanen Pertanian Vol 14, No 2 (2017): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v14n2.2017.98-107

Abstract

Mi merupakan makanan yang biasa dibuat dari terigu, tetapi juga dapat berasal dari beras, pati turunan kentang, singkong dan jagung. Secara umum, mi dibuat dari tepung, garam, air dan variasi bahan lain seperti bahan pengikat. Penelitian ini bertujuan untuk mengetahui pengaruh variasi penambahan bahan pengikat terhadap karakteristik sensori dan fisik mie mojang serta mendapatkan proporsi penambahan bahan pengikat yang tepat sehingga menghasilkan mie mojang dengan karakteristik yang baik. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) 1 faktor yaitu variasi penambahan bahan pengikat (telur, gum xanthan dan tepung konjak) dan dilakukan 3 kali pengulangan. Parameter sensori meliputi kesukaan terhadap warna, aroma, rasa, kekenyalan, penerimaan umum, dan sifat fisik (warna, tekstur, cooking loss dan daya kembang) merupakan parameter yang diamati untuk menentukan perlakuan terbaik. Produk terbaik dibandingkan dengan kontrol mie yang terbuat dari 100% terigu serta dilakukan analisis proksimat. Hasil uji efektifitas menunjukkan bahwa penambahan bahan pengikat konjak 0,75% merupakan perlakuan terbaik dengan penerimaan sensori meliputi kesukaan terhadap warna 3,5; aroma 3,0; rasa 2,9; kekenyalan 3,7; penerimaan umum 3,6; karakteristik fisik seperti chroma 29,51; Hue° 118,23; tekstur 14,475 kg/s2; cooking loss 11,62%; daya kembang 123,33%; dan karakteristik kimia meliputi kadar air 30,96%; abu 1,12%; lemak 0,79%; protein 2,95% dan karbohidrat 64,53%. Dengan demikian, nutrisi yang kaya tersebut dalam mi mojang akan sangat bagus sebagai sumber makanan instan.
Pengaruh Komposisi MOCAF (Modified Cassava Flour) dan Tepung Beras pada Karakteristik Beras Cerdas (Effect of Composition Mocaf (Modified Cassava Flour) and Rice Flour on Characteristics of Beras Cerdas) Achmad Subagio; Wiwik Siti Windrati
JURNAL PANGAN Vol. 21 No. 1 (2012): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v21i1.91

Abstract

Karakteristik Beras Cerdas sebagai produk yang menyerupai beras (beras analog) diteliti dengan menggunakan variasi komposisi bahan baku berupa MOCAF dan tepung beras. Beras Cerdas diproduksi dengan menggunakan teknologi ekstruksi dingin, dan hasilnya dikarakterisasi berdasarkan sifat kimia, fisik, dan organoleptiknya. Hasil penelitian menunjukkan bahwa semua formula Beras Cerdas mempunyai kadar air pada kisaran 9 persen (db) yang dapat menjamin daya simpan yang bagus. Kadar protein, lemak, abu dan karbohidrat Beras Cerdas dipengaruhi oleh formula perbandingan MOCAF dan tepung beras. Kadar protein cukup tinggi yaitu berkisar antara 7,2–9,7 persen. Sifat fisik beras cerdas yang meliputi derajat putih, daya kembang, bahan terdispersi, dandaya rehidrasi juga sangat dipengaruhi oleh formula perbandingan MOCAF dan tepung beras. Jika dibandingkan dengan beras biasa sebagai kontrol, nilai bahan terdispersi Beras Cerdas, tidak berbeda dengan beras (kontrol), yakni sebesar 6,23 persen ± 0,23. Uji kesukaan (preferensi) menunjukkan bahwa Beras Cerdas dengan MOCAF: tepung beras = 4:5 (formula B) mempunyai tingkat penerimaan keseluruhan yang terbaik (skor 3,44), dengan kelemahan pada rasa (skor 3,08).Characteristics of Beras Cerdas, the product resembles to ordinary rice (rice analog),are evaluated using the variation of raw material composition, i.e. MOCAF and rice flour. Beras Cerdas is produced by using cold extrusion technology. The product is then analyzed based on its physical, chemical, and sensory properties. The results show that all Beras Cerdas formulas have moisture content in the range of 9 percent (db) which ensures its best shelf life. Levels of protein, fat, ash and carbohydrates of Beras Cerdas are affected by the formula of MOCAF and rice flour, with a high protein content ranging from 7.2 to 9.7 percent. The physical properties of Beras Cerdas, which include the degree of whiteness, expansion power, the dispersed material, and the dehydration rate,are also greatly influenced by the ratio of MOCAF and rice flour. When the product is compared with the ordinary rice as a control, in terms of its dispersed material, it is found to be very similar to that of ordinary rice (control), i.e. in the order of 6.23 percents ± 0.23. Furthermore, the consumer preference test shows that Beras Cerdas with the ratio of MOCAF: rice flour = 4:5 (formula B) has the best overall acceptance rate (3.44), with the weakness in the taste (3.08).