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All Journal Journal of Science and Science Education (JoSSEd)
Ruth Rize Paas Megahati
STKIP PGRI Sumbar
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Materials Science & Nanotechnology Neuroscience Physics

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Thermozyme Amylase from Enterobacter sp Extremophiles in Bioethanol Production Gustina Indriati; Ruth Rize Paas Megahati
Journal of Science and Science Education Vol. 2 No. 1 (2021): April
Publisher : Pascasarjana, Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.931 KB) | DOI: 10.29303/jossed.v2i1.698

Abstract

Bioethanol is an alternative fuel to replace fossil fuels due to the reduction of fossil fuels. Bioethanol is produced from starch and converted to sugar by thermozyme amylase. Thermozyme amylase produced by thermophile bacteria can be obtained from Pariangan hot springs which have a temperature of  55 °C, pH of 9.2, and have a high level of bacterial diversity. The aim of this study was to obtained isolates of thermozyme amylase-producing bacteria that have the potential for bioethanol production. The research method were bacteria isolation, amylase stability test, temperature and pH optimization, and bioethanol production. The result showed that Enterobacter sp has been isolated from Pariangan hot springs is stable up to 5 hours of incubation, temperature and pH optimum was 85 °C, pH 8.5, and fructose as a carbon source. Thermozyme amylase converts starch into sugar under optimum conditions with a yield of 9.8% bioethanol
Co-Authors Gustina Indriati