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All Journal International Journal of Nasopharyngeal Carcinoma
Imam Prabowo
Department of Otorhinolaryngology – Head and Neck Surgery Faculty of Medicine Sebelas Maret University/ Moewardi Hospital Surakarta – Indonesia
Health Professions Medicine & Pharmacology

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THE RELATION OF EBNA-1 AND EGFR EXPRESSION SCREENING FROM ADVANCED STAGE UNDIFFERENTIATED NASOPHARYNGEAL CANCER Imam Prabowo; Agus Zuliyanto; Made Setiamika; Hadi Sudrajad; Aulia Hervi Anggraini; Dyah Ratna Budiani; Santoso Cornain; Made Nasar; Oyong
INTERNATIONAL JOURNAL OF NASOPHARYNGEAL CARCINOMA Vol. 1 No. 02 (2019): International Journal of Nasopharyngeal Carcinoma
Publisher : TALENTA PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1121.229 KB) | DOI: 10.32734/ijnpc.v1i2.1148

Abstract

Introduction: Nasopharyngeal carcinoma (NPC) is a lymphoepithelial malignancy of the nasopharynx, one of the etiologies is the infection of Epstein–Barr virus in undifferentiated type of nasopharyngeal carcinoma. The Epstein-Barr virus Nucleus Antigen-1 (EBNA-1) is Epstein–Barr virus-encoded proteins as regulatory virus transcription. Epithelial Growth Factor Receptor (EGFR) consist of a single polypeptide chain of amino acid, ErbB members, tyrosine kinase receptor, a transmembrane glycoprotein encoded by gen location in the short arms of a chromosome and overexpression in epithelial tumors. EGFR plays a central role in signal transduction pathways which regulate key cellular functions in epithelial malignancies. and may also present in NPC. Objective: To investigate this relation of expression patterns of EBNA-1 and EGFR in a histological type of undifferentiated nasopharyngeal carcinoma. Method: Observational, analytical study with a cross-sectional method of 34 formalin-fixed within inclusion criteria are EBNA-1 and positive staining of EGFR expression (30 patients) and exclusion criteria of negative staining of EGFR (4 patients). All biopsy samples work with paraffin embedded and resulted in hematoxylin-eosin undifferentiated histological types of the advanced stage in nasopharyngeal carcinoma. EBNA-1 and EGFR expression used immunohistochemistry staining. Result: EBNA-1 and EGFR expression level were detection and correlated with the advanced stadium of nasopharyngeal undifferentiated carcinoma. Conclusion: EBNA-1 is significantly related to EGFR expression in the advanced stadium of undifferentiated nasopharyngeal carcinoma. Overexpression of EGFR is mostly found in advanced NPC but not in all ages. Male is dominated and overall age below 55 years old. Screening of EGFR with immunohistochemistry is highly considered before anti-EGFR treatment
Effects of Cisplatin on Superoxide Anion, Sod, Apoptosis and Cytoplasmic p21Expression in C666-1 Cell Lines Undifferentiated Nasopharyngeal Cancer Imam Prabowo; Edi Widjajanto; Aris Widodo; Karisma Prameswari Patria Mahatmi; Handoko Nugroho Yossarsongko; Pudji Rahaju
INTERNATIONAL JOURNAL OF NASOPHARYNGEAL CARCINOMA Vol. 3 No. 02 (2021): International Journal of Nasopharyngeal Carcinoma
Publisher : TALENTA PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijnpc.v3i02.6343

Abstract

Introduction: ROS, a mechanism in CIS pathogenesis, causes toxicity when cisplatin is administered. Superoxide anion (O2-), a radical anion, part of ROS that is initially triggered after a part of oxygen enters a living cell. The main principle of this enzyme is to work as a protection against oxygen toxicity and plays a role as body catalyst. The p21 has a main function in the regulation of cell cycle progression. It is able to regulate cell proliferation through its association with PCNA and DNA polymerase accessory factor as a part of cell regulation and apoptosis. Objective: To study the effects of cisplatin on the increase or decrease of O2-, SOD, proliferation (MTT), apoptosis and cytoplasmic p21 expression in C666-1 cell lines. Methods: An experimental study with a post control group design and cisplatin in group dosages of 7.86 µg/mL, 15.36 µg/mL and 30.72 µg/mL. Results: The mean difference of O2- in C666-1 cell lines between cisplatin groups was not significant (P value 0.871, P>0.05). The mean difference of SOD in C666-1 cell lines was highly significant (P value 0.001, P<0.05) showing a significant increase of SOD. The mean difference of C666-1 cell lines proliferation (MTT) between cisplatin groups was not significant (P value 0.094, P>0.05). The mean difference of C666-1 cell lines apoptosis between cisplatin groups was not significant (P value 0.104, P>0.05). Cytoplasmic p21 highest expression was found at the 30.72 dosage obtained from 24 hours observation. Conclusion: Increase of SOD after a high dose of cisplatin administration, due to the balance between ROS production and detoxification process caused by antioxidants (SOD), will affect cancer cells to proliferate and survive. Increase of apoptosis at the highest dose found in this study, showed that apoptosis was induced by cisplatin. Therefore, antioxidant administration may be considered for nasopharyngeal cancer patients.
Co-Authors Agus Zuliyanto Aris Widodo Aulia Hervi Anggraini Dyah Ratna Budiani Edi Widjajanto Hadi Sudrajad Handoko Nugroho Yossarsongko Karisma Prameswari Patria Mahatmi Made Nasar Made Setiamika Oyong Pudji Rahaju Santoso Cornain