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Raditya Yoga
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ISOLASI DAN UJI ANTIRADIKAL BEBAS MINYAK ATSIRI PADA DAUN SIRIH (Piper betle Linn) SECARA SPEKTROSKOPI ULTRA VIOLET-TAMPAK I M. Oka Adi Parwata; Wiwik Susanah Rita; Raditya Yoga
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 1 Januari 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation, identification and free radical activities testing of essential oil from Piper betle Linn were carriedout. Around 13,0 mL (12, 37 gram) of yellow essential oil was obtained from 10,0 kg fresh leaves of Piper betleLinn. The oil was then partitioned with MeOH : H2O (7:3), n-hexane and chloroform.Free radical activities testing showed n-hexana fraction was the most active which can reduced 66,27 % ofDPPH for 5 minutes and 89,13 % in 60 minutes. GC-MS analysis revealed that there were 27 peaks. Based on thedata analysis there were only 9 compound, i.e. 4-metil(1-metiletil)-3-sikloheksen-1-ol, 1-metoksi-4(1-propenil)benzene, 4-(2-propenil)fenol/kavicol, 4-alilfenilacetate, Eugenol, Karyofilen, 3-alil-6-metoksifenilasetat, 4-alil-1,2-diasetoksibenzena dan dekahidro-4a-metil-1-metilen-7(1-metiletenil) naftalena.Based on peak’s intensity the oil were dominated by 4 compound i.e. 4-allyl phenyl acetate, 2 methoxy-4-(2 prophenil) fenol/eugenol, 3-allyl-6-methoxy phenyl acetate, 4-(2-prophenyl)-phenol / cavikol.
Co-Authors I Made Oka Adi Parwata WIWIK SUSANAH RITA