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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Indonesian Journal of Agricultural Science
Atsushi Yoshimura
Faculty of Agriculture Kyushu University
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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IDENTIFICATION AND MAPPING OF A GENE FOR RICE SLENDER KERNEL USING Oryza glumaepatula INTROGRESSION LINES Sobrizal, Sobrizal; Yoshimura, Atsushi
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

World demand for superior rice grain quality tends to increase. One of the  criteria of appearance quality of rice grain is grain shape. Rice consumers  exhibit wide preferences for grain shape, but most Indonesian rice consumers prefer long and slender grain. The objectives of this study were to identify and map a gene for rice slender kernel trait using Oryza  glumaepatula introgression lines with O. sativa cv. Taichung 65 genetic background. A segregation analysis of BC4F2 population derived frombackcrosses of a donor parent O. glumaepatula into a recurrent parent Taichung 65 showed that the slender kernel was controlled by a single recessive gene. This new identified gene was designated as sk1 (slender kernel 1). Moreover, based on the RFLP analyses using 14 RFLP markers located on chromosomes 2, 8, 9, and 10 in which the O. glumaepatula chromosomal segments were retained in BC4F2 population, the sk1 was located between RFLP markers C679 and C560 on the long arm of chromosome 2, with map distances of 2.8 and 1.5 cM, respectively. The wild rice O. glumaepatula carried a recessive allele for slender kernel. This allele may be useful in breeding of rice with slender kernel types. In addition, the development of plant materials and RFLP map associated with slender kernel in this study is the preliminary works in the effort to isolate this important grain shape gene.
Identification of a Notched Kernel Gene Associated with Pre-Harvest Sprouting Using Oryza glumaepatula Introgression Lines in Rice Sobrizal, ,; Yoshimura, Atsushi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.737 KB) | DOI: 10.24831/jai.v35i2.1312

Abstract

Pre-harvest sprouting in rice is related to the lack of a normal dormancy level during seed development and maturation. The prominent effects of pre-harvest sprouting are lower yield due to harvest losses and end-product quality reduction. A single novel gene (nk2) for notched kernel was identified at backcross segregating population (BC4F2) of Oryza glumaepatula to Oryza sativa cv. Taichung 65 as recurrent parent. The nk2 gene was closely associated to pre-harvest sprouting character, and mapped on the long arm of chromosome 5 with 3.5 cM and 3.6 cM distance to G1103 and R521 RFLP markers, respectively.   Key words :  Notched kernel, pre-harvest sprouting, genetic map, rice
Co-Authors , Sobrizal Sobrizal Sobrizal