Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Journal of Stem Cell Research and Tissue Engineering
deya karsari
Unknown Affiliation
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Genetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material deya karsari
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22748

Abstract

This study aims to  analyze   genetic stability of  the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged  in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.
Co-Authors