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All Journal 3BIO: Journal of Biological Science, Technology and Management Journal of Integrated and Advanced Engineering (JIAE)
Khalilan Lambangsari
Department of Bioengineering, School of Life Sciences and Technology, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Neuroscience

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Analysis of Plant Growth and Gallic Acid Content for Cavendish Banana (Musa acuminata) Shoot Culture with Bubble Column Bioreactor Nunung Nurhayati; Rizkita Rachmi Esyanti; Khalilan Lambangsari
Journal of Integrated and Advanced Engineering (JIAE) Vol 2, No 1 (2022)
Publisher : Asosiasi Staf Akademik Perguruan Tinggi Seluruh Indonesia (ASASI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51662/jiae.v2i1.37

Abstract

Cavendish banana (Musa acuminata) is one of the most important fruits in the world. Cavendish shoots tissue culture using bubble column bioreactor can be a solution to produce high yielding plantlet and gallic acid due to the aeration with minimum shear stress. In this study, the average growth rate, presence of gallic acid, and antioxidant activity (IC50) in the bubble column bioreactor (200 mL capacity) with the aeration rates of 1 mL/s and 2 mL/s using Murashige & Skoog half-strength liquid medium supplemented with 0.5 ppm gibberellic acid will be analyzed. The aeration system used was atmospheric air. The leaves and stems were extracted by maceration using 96% ethanol solvent (1:10 (w/v)). A qualitative phenolic test with FeCl3, thin layer chromatography, and antioxidant test with 2,2-diphenyl-1-picrylhydrazyl was carried out. The average growth rate in the bioreactor were 0.22 ± 0.001 g/day (1 mL/s) and 0.21 ± 0.001 g/day (2 mL/s). All the leaf and stem extracts showed positive results for the phenolic test, but the presence of gallic acid could not be detected clearly by thin-layer chromatography. The IC50 values in aeration rates of 1 mL/s and 2 mL/s of the leaves were 41.35 and 79.54 μg/mL, respectively, while the stems were 51.87 and 104.94 μg/mL, respectively. It could be concluded that the growth of the banana plantlet and the production of antioxidants in the bubble column bioreactor was higher in aeration rate of 1 mL/s than 2 mL/s.
An Evaluation of Stevia rebaudiana Leaf-Axillary Shoot Formation, Cultured in MS Medium Supplemented with IAA-BAP and MS Medium Supplemented with Kinetin Andira Rahmawati; Victor Emmanuel; Iriawati Iriawati; Khalilan Lambangsari; Rizkita Rachmi Esyanti; Roohaida Othman; Aldo Nathan Dela Simamora; Bugy Suwito
3BIO: Journal of Biological Science, Technology and Management Vol. 5 No. 1 (2023)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2023.5.1.1

Abstract

Stevia rebaudiana leaves can be used as a sweetener alternatives because they contain steviol glycoside derivative compounds, including steviosides and rebaudioside-A. Propagation of Stevia is more optimally carried out using in vitro culture when compared to conventional propagation through seeds or cuttings. This study aimed to evaluate the formation and growth of Stevia shoots and leaves in MS medium containing a mixture of IAA and BAP with MS medium containing kinetin only, as well as evaluating the use of a liquid medium containing kinetin. Stevia was initiated from apical shoot then grown in MS medium containing a mixture of IAA and BAP with MS medium containing kinetin only. Stevia was subcultured every 4 weeks. Several parameters measured were number of axillary shoots and number of leaves. It was transferred into a liquid medium for 7 days. The results showed that the formation and growth of axillary buds and leaves at the initiation stage were better in medium containing IAA and BAP compared to medium containing single hormone kinetin. At the stage of shoot multiplication and maintenance, cultivation in semi-solid medium containing kinetin showed more leaves and axillary shoots compared to that cultivated in semi-solid medium with the addition of IAA and BAP. Plants acclimatized in liquid medium supplemented with 1 ppm kinetin showed fast plant growth but were not accompanied by sturdy stem growth. The presence of brownish color on certain parts of the plant such as in some leaves and stems was also observed. 
Co-Authors Aldo Nathan Dela Simamora Andira Rahmawati Bugy Suwito Iriawati Iriawati Nunung Nurhayati Rizkita Rachmi Esyanti Rizkita Rachmi Esyanti Roohaida Othman Victor Emmanuel