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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Zuriat
, Zulkarnain
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education

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The Production of Tetraploid Swainsona formosa by Colchicine Mutagenesis , Zulkarnain
Zuriat Vol 15, No 1 (2004)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v15i1.6879

Abstract

Swainsona formosa, a legume native to Australia, is a spectacular flowering plant that is used as a hanging basket, container or cut flower plant. Its breeding programme had been emphasized on the production of better flower quality through ploidy modification to produce tetraploid plants. Colchicine, an antimitotic chemical, had been sucessfully used to induce tetraploidyzation in S. formosa. Up to 50% of plants with double chromosome number (2n = 4x = 32) were produced by seedling application of colchicine at 0.01%–0.1%. Concentrations below 0.01% or above 0.1% were found not effective in inducing chromosome doubling. Tetraploid S. formosa were charaterized by larger leaflets, bigger size but lower density of stomata, larger flowers with longer peduncles and larger pollen grains with lower viability. In general, colchicine can be used effectively to induce polyploidyzation in S. formosa and this method should find application in breeding programme.
RESEARCH NOTE Floral Bud Length as Morphological Predictor for Microspore Developmental Stage in Sturt’s Desert Pea (Swainsona formosa) , Zulkarnain
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.727 KB) | DOI: 10.24831/jai.v33i2.1520

Abstract

This work was conducted to establish the relationship between microspore developmental stage and length of the floral bud in glasshouse-grown Sturt's desert pea, a native Australian legume.  The stages of microspore development were segregated into tetrad, early-uninucleate, mid-uninucleate and late-uninucleate.  The results showed that the stage of microspore development was highly dependent on the length of floral bud.  The tetrad stage lasted longer than early-, mid- or late-uninucleate stages.  The attempted induction of androgenesis in Sturt's desert pea using anthers from floral buds with similar size, as in the present work, was unsuccessful.  However, our work showed that the floral bud length can be used as a reliable predictor of microspore developmental stage in Sturt's desert pea.   Key words: Sturt's desert pea, Swainsona formosa, androgenesis, legume
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