Sujono Sujono
Jurusan Analis Kesehatan Poltekkes Kemenkes Yogyakarta

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Media Agar Tepung Kacang Hijau, Kacang Merah, Kacang Tunggak, Kacang Kedelai Sebagai Media Kultur Jamur Aspergillus Flavus Anik Nuryati; Sujono Sujono
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 13 No. 1 (2017): Maret
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Sabouraud Dextrose Agar (SDA) is a medium for the growth of fungi, ready to use, packaged in 500 gr, expensive, hard to find and takes a long time to obtain so it needs alternative media. SDA contains 4% glucose. The use of mung bean, red bean, cowpea and soy bean flour as culture media has never been done. The material contains high protein and high vegetable oil, allowing Aspergillusflavus to grow in it. The research aimed to know whether mung bean , red bean, cowpea, soy bean flour agar media may be used as culture media on the growth of Aspergillus flavus, and to know the diameter and color of colonies. Subjects were Aspergillusflavus fungus, inoculated in SDA media with incubation time 24 hours. Macroscopic shape: filamentous colonies, clear. Microscopic: insulated hyphae, branching, there are vesicles with conidia arranged like a fan. The object of the research are mung bean, red bean, cowpea, and soybean flour obtained from the manufacture of good quality peanut flour, whole seeds, not rancid, not wormy. Method: This research is an experimental study. The treatment was given to the independent variable and then measures the dependent variable. The result shows that mung bean, red bean, cowpea, soy bean flour agar media can be used as culture media on the growth of Aspergillusflavus fungus. Diameter of colony growth to five days on soybean flour agar medium was 7.1 cm, it was greater than red beans media 6.1 cm, mung bean media 6.7 cm and SDA 6.5 cm. Colony color on all media are white, turns yellow and then green and dark green until the end. It can be concluded that Aspergillus flavus fungus can grow on mung bean, red bean, cowpea, soy bean flour agar media with various diameters.
Perbedaan Kadar Kreatinin Pada Serum Lipemik Yang Diolah Dengan Polyethylene Glycol 6000 8% Dan High Speed Sentrifugasi Wheny Mufita Sari; Ni Ratih Hardisari; Sujono Sujono
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 13 No. 1 (2017): Maret
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (864.346 KB)

Abstract

Lipemic serum is a turbid serum caused by enhancement of lipoprotein consentration mainly chylomicron and very low density lipoprotein (VLDL). It can cause interference of physics and chemistry, spectrofotometri, not homogen sample and volume displacement effect that make the result of the laboratory test inacurate, including creatinin test. The method that can be used to clear up lipid on the lipemic serum is centrifugation, extraction with polar solvent, dilution, and precipitation with Polyethylene Glycol 6000 8%. The aim of the study is to know creatinine level defference of lipemic serum using Polyethylene Glycol 6000 8% in creatinine test. This research was a true experiment with Pretest Postest Control Group Design. This research was conducted in October – November 2016 at Pathology Clinic of Panembahan Senopati Hospital, Bantul. The result of creatinine scores before treatments was 0,98 mg/dL, after being added with Polyethylene Glycol 6000 8% it became 0,98 mg/dL, and after being processed using High Speed Centrifugation, it was 1,11 mg/dL. The result of statistic test using Independent Sample T-Test obtained the Asymp sig value of 0,003 (p<0,05) which means there is no difference of creatinine scores in lipemic serum processed by adding Polyethylene Glycol 6000 8% and High Speed Centrifugation.