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All Journal Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Jurnal Akademika Biologi Pro-Life
Agung Janika Sitasiwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics

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PENENTUAN KANDUNGAN ESTRADIOL (E2) DAN LUTEINIZING HORMONE (LH) PADA PETAURUS BREVICEPS PAPUANUS (MARSUPIALIA) SELAMA SATU STKLUS ESTRUS. Lieu A; Sutasurya N; Agung Janika Sitasiwi
Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Vol 2, No 2 (2001): Agustus 2001
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jstni.2001.2.2.1673

Abstract

PENENTUAN KANDUNGAN ESTRADIOL (E2) DAN LUTEINIZING HORMONE (LH) PADA PETAURUS BREVICEPS PAPUANUS (MARSUPIALIA) SELAMA SATU STKLUS ESTRUS. Telah dilakukan penentuan kandungan estradiol (E2) dan Lueinizing hormone (LH) plasma darah selama satu siklus estrus pada Petarus breviceps papuanus betina dewasa, stuatu marsupialia yang hidup di Irian Jaya dan Kepulauan Aru. Data biologi reproduksi jenis hewan ini sangat sedikit. Kadar hormon E2 dan LH plasma darah pada setiap fase selama sikius estrus ditentukan dengan metode RIA. Hasil penelitian menunjukkan bahwa kandungan hormon E2 dan LH mengalami kenaikan dan penurunan secara nyata dan berjalan seiring dengan dicapainya perubahan fase selama sikius estrus. Kandungan hormon E2 dan LH tertinggi dicapai pada fase estrus. Sedang kandungan terendah pacla Pasediestrus. Padafase estrus. LH memperlihatkan terjadinya lonjakan konsentiasi yang berarti bahwa pada periode estrus ¡ni terjadi ovtilasi. Akan tetapi, Pada penelitian ini, waktu terjadinya ovulasi belum dapat ditentukan. Dapat disimpulkan bahwa kandungan E2 dan LH pada Petarus breviceps papuanus betina dewasa selama satu siklus mencapai kandungan tertinggi pada fase estrus dan terendah pada fase diestrus 
HEPATOSOMATIC INDEX (HSI) DAN DIAMETER HEPATOSIT MENCIT (Mus musculus L.) SETELAH PAPARAN EKSTRAK AIR BIJI PEPAYA (Carica papaya L.) Perwitasari Wahyuningtyas; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.951 KB)

Abstract

Papaya seeds were one part of the papaya plants that can be used as a traditional medicine. The purpose of this study was to test the effect of papaya seed extract (Carica papaya L.) to Hepatosomatic Index (HSI) and hepatocytes diameter of mice (Mus musculus L.). This study was an experimental study use Completely Randomized Design (CRD) that divided into 5 treatment groups with 3 replications. P0 test material in the form of aquadest, P1, P2, P3 in the form of papaya seeds extract test with dose respectively 1.4; 3.5; And 7 mg / mL. Treatment were administered orally for 21 consecutive days. The Body weights of mice were measured every 7 days. After treatment were terminated, the body weights of mice were measured and then sacrificed by dislocation of the neck and the liver were taken by surgery. Furthermore, the liver weights were measured and made histological preparations with HE staining. The determination of HSI were obtained from liver weights and body weights. The measurement of hepatocytes diameter were done by using photomicrograph at each incision 3 field of view. Data were analyzed by Analysis of Variance (ANOVA) at 95% confidence level. The analysis result of HSI in the treatment group showed that there were no significant difference (p>0,05). The analisys of hepatocytes diameter of the treatment group showed significant difference (p<0.05). It could be concluded that exposure of papaya seeds extract for 21 days was a reversible effects so that the hepatocytes diameter size of mice increases but didn’t changed the HSI.Keywords: Carica papaya, HSI, hepatocytes diameter
EFEK EKSTRAK ETHANOL DAUN MIMBA TERHADAP GONADOSOMATIC INDEX (GSI), JUMLAH DAN UKURAN FOLIKEL ATRESIA PADA MENCIT (Mus musculus L.) BETINA Nurul Hidayah; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Pro-Life Vol. 5 No. 2 (2018): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Neem leaves contain flavonoid, triterpenoid and saponin compounds that have antifertility potential to suppress of reproduction rate of rodentia pests. The purpose of this study was to examine the effect of neem leaf ethanol extract on gonadosomatic index (GSI), number and size of atresia follicles in female mice. This study used a Completely Randomized Design Method consist of 5 treatments and 4 replications namely K + (Contraceptive Pills); K- (Aqua); P1, P2 and P3 (Treatment of neem leaf ethanol extract at dosage of 8.4 mg / kgBW, 11.2 mg / kgBW and 14 mg / kgBW). The treatment was administered orally at a volume of 0.2 ml / animal every morning for 21 days. Measurement of body weight was conducted at the beginning of treatment and every 7 days. The ovaries were isolated, weighed and histologically processed using paraffin method and H & E staining. the number and diameter of atresia follicles were counted on ovaries. The data was analyzed using ANOVA test and DMRT test at 95% confidence level. The results showed that neem leaf ethanol extract had no significant effect GSI and diameter of atresia follicle but had significant effect on the number of atresia follicle. It can be concluded that neem leaf ethanol extract can increase the number of atresia follicle in mice by suppressing the synthesis and action of reproductive hormone. Keywords: mimba leaf, atresia follicle, GSI, mice
Co-Authors Cecep Kusmana Lieu A Perwitasari Wahyuningtyas Siti Muflichatun Mardiati Sutasurya N