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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI)
IS HELIANTI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : hayati journal of biosciences

 1 
Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring IS HELIANTI
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.564 KB) | DOI: 10.4308/hjb.14.2.54

Abstract

A functional gene containing an Open Reading Frame (ORF) encoding a ?-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application. Key words: ?-1, 4-endoxylanase, metagenomic DNA, Pawan-Riau hot-spring
Co-Authors . KOESNANDAR AA Sudharmawan, AA Abinawanto Abinawanto AGUSTIN KRISNA WARDANI Ani Suryani ASEP RISWOKO ASTUTIATI NURHASANAH Budiasih Wahyuntari Dea Indriani Astuti DEWI NANDYAWATI Dini Achnafani DWI SURYANTO FENTRI PARAMITHA PUTRI Haniyya Haniyya Joni Kusnadi Khaswar Syamsu Lisa Pratama LLINA MULYAWATI MILANI ANGGIANI Mochamad Nurcholis Muhamad Taufiqul Naufal NIKNIK NURHAYATI RIMA AZARA Sara Gustia Wibowo SHANNI FERNANDA TATI NURHAYATI