Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Microbiology Indonesia Proceeding International Conference on Global Resource Conservation
DHIRA SATWIKA
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Molecular Detection of Enterobacteriaceae and Escherichia coli from Water Hilson, Eunike Ilona; Prana, Mertha; Satwika, Dhira
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Abstract

Well water has become the important source for public daily activities, especially as drinking water. Enterobacteriaceae is a bacterial group, often found in well water. There are 8 genus of Enterobacteriaceae consider as enteric group because of its relationship to fecal bacteria from human and animal gastrointestine.  Escherichia coli, a member of this group, is one of the best microbiological fecal indicator that have been used for water monitoring. The presence of this bacterial group is of human interest, especially related to their pathogenic characteristic and as the fecal indicator for water quality. This is the objective of this research to evaluate the specivity of new primers which were deduced from partial sequences of rpoB, gyrB and lacZ genes specifically designed for Enterobacteriaceae and E. coli detection. After being optimized by mean of setting the annealing temperature, they are used for in vitro analysis. The result showed the primers are specific enough for Enterobacteriaceae and E. coli detection.Keywords:  Enterobacteriaceae, E. coli, gyrB, lacZ, rpoB
Molecular Phylogeny of Salmonellae: Relationships among Salmonella Species Determined from gyrA, gyrB, parC, and parE Genes CHARIS AMARANTINI; DHIRA SATWIKA
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.474 KB) | DOI: 10.5454/mi.9.1.1

Abstract

Study on molecular characteristics of Salmonella from clinical isolates was done in order to find out its relationship, especially those isolated from Indonesia. Partial sequence of genes belonging to QRDR region, i.e. gyrA, gyrB, parC, and parE were employed. Specific primer pairs covering those genes are used to amplify the bacterial DNA obtained. The amplicons were then analyzed by means of sequencing, and the sequences are analysed bioinformatically to find out similarities and build phylogenetic trees. By comparing all of the phylogenetic tree from QRDR region, this study revealed gyrA as the most suitable gene for rapidly identify member of salmonellae as it gives better separation of samples being analysed. However, the use of parC is recommended as it gives a consistent and reliable value to separate member of Salmonella and other Enterobacter. Further studies are under way to include member of this group, like E. coli, and the use of full sequence of QRDR genes region to verify this report.
Co-Authors CHARIS AMARANTINI Eunike Ilona Hilson, Eunike Ilona Mertha Prana, Mertha