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Erni Martani

Unknown Affiliation

Author-ID : 435201

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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1

Superoxide Dismutase of Micrococcus sp. S2 and Its Involve in Paraquat Detoxification Sebastian Margino; Erni Martani; Medhina Magdalena
Indonesian Journal of Biotechnology Vol 12, No 1 (2007)
Publisher : Universitas Gadjah Mada

As an active ingredient of herbicide, paraquat will induce formation of superoxide radicals. The previousresearch succeeded in isolating paraquat degrading bacteria from peat soil, Micrococcus sp. S2, that tolerant to highconcentration of paraquat. An anti-oxidative enzyme, namely superoxide dismutase (SOD, EC.1.15.1.1), wasbelieved to be responsible for the paraquat tolerance. This research was conducted to study the characteristic of theSOD synthesize by Micrococcus sp. S2 and its ability on neutralize superoxide which arise from paraquat reoxidation.To observe the effect of paraquat on Micrococcus sp. S2, the bacteria was grown in 10% Luria Bertani brothmedium amended with several concentrations of paraquat, from 0 (control) up to 100 mg/ml. Within incubationtime of 72 hours, bacterial growth, activity of superoxide dismutase and paraquat residue were analyzed. Theisozymes of superoxide dismutase were distinguished using two kinds of specific inhibitor, namely HO and KCN. 2 2The results showed that paraquat significantly inhibit the growth of Micrococcus sp. S2. The higher paraquatcocentration in the medium caused the higher growth inhibition. However, the bacteria is still survive in the mediumcontaining toxic herbicide, and this ability was suggested related to superoxide dismutase activity in removing thesuperoxide radicals. Analysis using gel electrophoresis indicated that at least three types of SOD isozyme weresynthesized by Micrococcus sp. S2; they were Ferri-SOD (Fe-SOD), Mangani-SOD (Mn-SOD), and the last one wassuspected to be the Cupro Zinc-SOD (CuZn-SOD). The Mangani-SOD was suspected to play an important roles ondetoxifying superoxide which arise from paraquat oxidation.Keywords : Micrococcus sp.S2, paraquat, superoxide dismutase, isozymes

Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste Sebastian Margino; Erni Martani; Andriessa Prameswara
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB ispotential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. Thedevelopment of PHB production using starch as an alternative carbon source has been conducted to reducethe dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processingsite were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of thestudy showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB rangedfrom 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 andafter optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubationtime. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed asMicrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicatedas poly-β- hydroxybutyrate (PHB)

Isolasi dan Seleksi Bakteri dari Sedimen Mangrove untuk Pembentukan Konsorsium Bakteri Perombak Dibenzofuran Yanisworo W. Ratih; Bostang Radjagukguk; Erni Martani; Irfan D. Prijambada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.115

Dibenzofuran merupakan salah satu senyawa hidrokarbon aromatis polisiklik (HAP) yang mengandung oksigen. Paparannya di alam harus segera ditanggulangi karena dibenzofuran berperan sebagai prekursor bagi senyawa berkhlor turunannya yang bersifat lebih toksik. Dibenzofuran dapat dijadikan senyawa model karena beberapa bakteri perombak dibenzofuran juga mampu merombak senyawa mirip lainnya seperti dibenzodioksin, fluorena, fluorantena, dibenzofuran terkhlorinasi, fenantrena dan antrasena. Penelitian ini dilakukan untuk membentuk konsorsium bentukan yang mempunyai kemampuan tinggi dalam merombak dibenzofuran. Isolat bakteri diperoleh dari sedimen mangrove asal Balongan, Indramayu, Jawa Barat menggunakan medium mineral cair yang diperkaya dengan dibenzofuran sebagai satu-satunya sumber karbon dan energi. Total 12 isolat bakteri, GMYk-1, GMYk-2, GMYk-3, GMYk-4, GMYk-5, GMYs-1, GMYs-2, GMYs-3, GMYs-4, GMYs-5, GMYs-6 dan GMYs-7 berhasil diisolasi dari sedimen. Berdasarkan pengamatan terhadap keragaman isolat-isolat yang diperoleh serta interaksi di antara isolat dalam merombak dibenzofuran, empat isolat berhasil terseleksi untuk menyusun konsorsium bentukan. Isolat tersebut adalah GMYs-1, GMYs-6, GMYs-7 dan GMYk-1. Berdasarkan kemampuan merombak dibenzofuran dari kombinasi isolat-isolat yang disusun, biakan campuran GMYs-1- GMYs-6-GMYk-1 dipilih sebagai konsorsium bentukan. Konsorsium bentukan mempunyai kemampuan paling tinggi dalam merombak dibenzofuran.

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