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All Journal Prosiding Biosaintifika: Journal of Biology & Biology Education
Tata Brata Suparjana
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Education

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KARAKTERISTIK MORFOLOGI DAN POLA PITA IZOSIM VARIETAS MANGGA (Mangifera sp.) DI KABUPATEN BANYUMAS , Sumarsono; Suparjana, Tata Brata; Purwati, Endang Sri
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Mangga (Mangifera indica) merupakan salah satu tanaman hortikultura yang memiliki nilai ekonomi tinggi. Untuk membedakan antara varietas mangga, penanda genetik yang digunakan karena mereka tidak dipengaruhi oleh faktor lingkungan. Salah satu penanda genetik yang sering digunakan adalah Isozim. Tujuan penelitian ini adalah untuk mengetahui pola bandeng dan variasi genetik beberapa varietas mangga dari Kabupaten Banyumas berdasarkan peroksidase isozim (PER), aspartate aminotransferase (AAT), esterase (EST), asam fosfatase (ACP). Penelitian ini dilakukan di Pabrik Laboratorium Biologi, Biologi Pusat Penelitian Ilmu, IPB, Bogor dari Juni sampai September 2012. Sampling daun dari tujuh varietas mangga dilakukan dengan purposive random sampling. Setelah eletrophoresis dan scoring, data kemudian dianalisis dengan Unweighted Pair-group Method with Arithmetic (UPGMA) under Numerical Taxonomy and Multivariate Sistem (NTSYS) version 2.20i. Hasil penelitian menunjukkan bahwa EST memiliki empat pola pita yang bermigrasi anodally. AAT memiliki lima pola banding dan ACP memiliki lima pola pita yang bermigrasi anodally. PER memiliki lima pola pita yang bermigrasi anodally dan katodally, MG4 (lokal dari indramayu mangga) tidak muncul. PER, AAT, ACP dan ACP menunjukkan pola yang berbeda dari dendogram. Kombinasi dari empat isozim menunjukkan bahwa variasi genetik antara MG4 (mangga lokal indramayu) dan MG5 (mangga lokal dari gedonggincu) adalah 79%, MG1 (lokal dari golek mangga) dan Mg3 (mangga lokal arummanis) adalah 75 %.
Mahameru Soybean (Glycine max) Cultivar, High Salinity Tolerant Juwarno, Juwarno; Suparjana, Tata Brata; Abbas, Muachiroh
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11870

Abstract

Mahameru cultivar is high salinity tolerant cultivar. The previous study result showed Mahameru cultivar could tolerate 140mM NaCl, but Cilacap Coast salinity levels often reaching 200mM NaCl. A research of salinity stress on Mahameru cultivar at 200 mM NaCl have not conducted yet. Therefore to conduct the research of Mahameru at high salinity stress to obtained high salinity tolerant soybean cultivar.   The observed variables are anatomy (epidermis thickness, the density of stomata and trichomes, palisade thickness) physiology (the dry weight of roots and canopy, the content of chlorophyll a and b) Production (whole pod, total filled pod, total empty pod, weight per one-hundred beans). The salinity treatment was 0, 50,100, 150, 200 mM NaCl given at three days before planting and twenty-one days after planting. The data of anatomy and physiology was taken at forty-five days after planting. The production data was taken when soybean plants turned brown. The result indicates that salinity affects anatomy characteristic of leaf, higher the salinity increasing epidermis thickness and the density of stomata and trichomes. Salinity affected the content of chlorophyll a and b. Higher the salinity increased the content of chlorophyll a and b. Salinity did not affect soybean production. Based on this study Mahameru cultivar is resistant to salinity up to 200 mM NaCl. The benefit of this research help to enhance national soybean production with utilization coastal land for soybean planting Mahameru cultivar.         
Co-Authors Abbas, Muachiroh Endang Sri Purwati Juwarno, Juwarno Sumarsono