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M W Lestari
Faculty of Agriculture, Malang Islamic University, Jl. Mayjen Haryono 195, Malang 65144, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Morphological characteristic of purple long yard bean cultivars and their tolerance to drought stress Lestari, M W; Kuswanto, K; Wardiyati, T; Widoretno, W
Journal of Degraded and Mining Lands Management Vol. 2 No. 2 (2015)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2014.022.281

Abstract

The cultivation of purple long yard bean which tolerance to drought stress and have high productivity can improve farming in arid area. The purpose of this study was to evaluate the mechanism of the tolerance purple long yard beans to drought stress based on morphologic characters, to get the hypothesis method of tolerance and to obtain tolerance cultivars to the drought stress. Eight cultivars of purple long yard beans, i.e. UBPHU1-41, UBPHU1-130, UBPU3-153, UBPU1-202, UBPU2-222, UBPU1-365, Brawijaya 4 and Bagong 2, were tested in two environmental conditions, 100% field capacity and 50% field capacity. The results showed that drought stress in purple long yard bean affected all morphological characters observed, except for root length and flowering time. Estimation of tolerance to drought stress using the Principles Component Analysis (PCA) showed that the shoot fresh weight could be an indicator of purple pod bean tolerance to drought stress. However, the test using Stress Susceptibility Index (SSI) was not able to classify the purple long yard bean tolerance to drought stress. The results of analysis using PCA followed by discriminant analysis and clustering dendrogram showed that the UBPU1-41, UBPU1-130, UBPU2-222, UBPU1-365, UB4 and Bagong 2 cultivars were medium cultivars that are tolerant to drought stress. Therefore, they can be planted in semiarid regions.
Isolation of Indigenous Bacteria of Phosphate Solubilizing from Green Bean Rhizospheres Arfarita, N; Lestari, M W; Murwani, I; Higuchi, T
Journal of Degraded and Mining Lands Management Vol. 4 No. 3 (2017)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2017.043.845

Abstract

The use of phosphate-solubilizing bacteria (PSB) as a biological fertilizer of Agricultural land is one solution to overcome problem of phosphate availability for plants. However, often application of a biological fertilizer is ineffective for certain places. The purpose of this study was to obtain indigenous phosphate solubilizing bacterial isolates that can be effective in the area of Malang. Samples were collected from rhizosphereof green bean plants at three locations in Malang, East Java. The study was conducted to determine the total bacterial population of soil samples, to select the best three bacterial isolates in phosphate solubilizing ability, which is not antagonistic and nonpathogenic for plants,along with observing its potential as a bacterial consortium. The highest total population was found in FHR samples of 1.5x1011 CFU / mL. We have selected three bacterial isolates namely SPP1, SPP2 and SPP3. They were not antagonistic to each other and nonpathogenic on mungbean sprouts. They had possibility of producing growth hormone which characterized by an increasing in length of plant and total root length, be compared to controls. Strain SPP2 has shown the highest activity of phosphate solubilization then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SPP2 had 99% similarity with Pseudomonas plecoglossicida strain PR19
Co-Authors K Kuswanto Murwani, I N Arfarita T Higuchi T Wardiyati W Widoretno