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Chomsin Sulistiya Widodo
Jurusan Fisika Fakultas MIPA Universitas Brawijaya
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering

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Journal : Natural B

 1 
Determination of Magnetic Properties of Drugs and Balanced Wastes Based on the Fluorescence Resonance Energy Transfer (FRET) Phenomenon Dina A Chusniyah; Djoko H. Santjojo; Chomsin S. Widodo
Natural B, Journal of Health and Environmental Sciences Vol 2, No 3 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.177 KB) | DOI: 10.21776/ub.natural-b.2014.002.03.6

Abstract

Along with population growth, the need for clean water is increasing. The availability of clean water is starting to decrease due to the decline in quality and quantity of water in the environment. Water quality degradation can be caused by water pollution from industrial waste disposal. One of the parameters to measure the level of pollution is the biochemical oxygen demand (BOD). Beside titration, biosensor method is a method developed to measure the BOD. The purpose of this study is to design a microorganism-based biosensor that can be used to measure levels of Biochemical Oxygen Demand (BOD5) using a microelectrode transducer with Pseudomonas putida and Pseudomonas fluorescents as bioreceptor on phenol wastes. BOD concentration was controlled by adding phenol in water with concentration of 0 mg/L, 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L. BOD concentration as measured by using titration method and developed biosensor. Experimental result shows that the potential of the biosensor linearly correlated to the concentration of BOD in water. Membrane surface area does not contribute any effect to the output potential of the biosensor. We can conclude that both Pseudomonas putida and Pseudomonas fluorescents can be use as material for measuring BOD concentration in water. 
Effect of Rosella Petal Extract (Hibiscus sabdariffa L.) On SOD Activity and Mouse Eye Rat (Rattus Wistar) Exposed to Gamma Radiation Rara Pramita; Chomsin S. Widodo; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.225 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.13

Abstract

Measurement activity of superoxide dismutase (SOD) and Malondhyaldehyde (MDA) to analyze the effect of roselle calyx extract (Hibiscus sabdariffa L) after exposure 300 rad gamma radiation. 35 male white rats (Rattus Wistar) were exposed to fractination dose of 300 rad for 7 day, are given in the sonde 2cc/days roselle extract for 16 days with a dose variation 62.5 mg/200 g BW, 125 mg/200 g BW, 250 mg/200 g BW, 400 mg/200 g BW, and 550 mg/200 g BW. Measurement of the activity of SOD and MDA were done by thiobarbituric acid (TBA) methode. Extract Roselle (Hibiscus sabdariffa L) 250 mg/200 gr BW increase the activity of superoxide dismutase (SOD) and decrease Malondialdehyde (MDA) after being exposed to radiation of 300 rad.
Studies on the Effect of Pollutant Waste on Free Radical Content in Tilapia Gill Organs (Oreochromis Niloticus) Nindha A. Berlianti; Chomsin S. Widodo; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.771 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.10

Abstract

Studies on the effect of heavy metal pollution on the appearance of free radicals in aquatic biota has been done by applying heavy metals Pb, Cd and Hg in tilapia fish (Oreochromis Niloticus). Free radicals research was done to organ of tilapia gills using ESR. The result showed that the concentration of Pb Nitrate 0.2 ppm – 1.4 ppm, Cd Nitrate 0.02 ppm – 0.14 ppm and Hg Nitrate 0.006 ppm – 0.018 ppm could improve free radical O2-. Free radicals concentration in organ of tilapia gills will increase with increasing concentration of heavy metals.
Effect of Gamma Radiation and Temulawak Extract (Curcuma xanthorrhiza) on HAART Levels of Mice Mice (Mus musculus) Septiana K. Sari; Chomsin S. Widodo; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 3, No 2 (2015)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.279 KB) | DOI: 10.21776/ub.natural-b.2015.003.02.12

Abstract

Gamma radiation can causes cells damage, because the interaction between radiations with cells can produce free radical. The compound that can neutralize and catch free radical is called antioxidant. The objectives of this research were to analyze influence of gamma radiation and Curcuma xanthorrhiza extract to the SGPT level to the mice’s liver. This research uses eighty mices and they were divided into three group, negative control (K-), radiation non extract (R-) and extract plus radiation (R+). The doses of treatment of Curcuma xanthorrhiza extract are 1.4 gr/kg body weight; 2.0 gr/kg body weight; 2.6 gr/kg body weight; 3,2 gr/kg body weight and 3.8 gr/kg body weight, while the exposure of gamma radiation are given during 10 minutes, 20 minutes, 30 minutes, 40 minutes and 50 minutes. The measuring of SGPT level in mice’s serum is done by using blood analyzer. The results showed that free radical can damages liver cells and breaks cell wall, so SGPT level in blood to be high. In a row of increasing of Curcuma xanthorrhiza extract dose, then SGPT level to be low. 
PFRET Measurements Using Sensitized Emission Methods in Observation of Calcium Changes in Oocytes Nathania Nanasari; Djoko Herry Santjojo; Chomsin Sulistiya Widodo; Hari Soepriandono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.525 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.5

Abstract

Fluorescence Resonance Energy Transfer (FRET) is a process of non-radiative energy transfer from donor molecule to an acceptor. We expect to know the quantitative FRET measurements in immature and mature goat oocyte with the presence of calcium. FRET was detected using a laser scanning confocal microscope. We assume that calcium as a donor and fluo-3 as an acceptor. The methods of sensitized emission have been used for quantitative FRET measurement. We also describe the precision FRET (PFRET) data analysis. The value of PFRET describe the presence of calsium in immature and mature oocytes. For these immature and mature oocytes, the results of PFRET are 622,5 and 754,57 respectively. Mature oocyte has more calsium than immature oocyte.
Analysis of Effect of Sound Frequency Against System Open Close Stomata of Rice Plant of Logawa Variety Kuni Nadliroh; Chomsin S. Widodo; Didik R. Santoso
Natural B, Journal of Health and Environmental Sciences Vol 3, No 2 (2015)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.515 KB) | DOI: 10.21776/ub.natural-b.2015.003.02.13

Abstract

This study aimed to determine the effect of sound waves to open the closed stomata rice plants in sonic bloom technology, the frequency used for rice crops, as well as help the intensification of agriculture. This study was conducted on rice types Logawa, where rice plants used are rice plants ready for planting. Ready for planting rice plants treated without wave, 10 Hz, 4 kHz, 7 kHz, 30kHz. Parameters measured were wide rice plant stomata. The results showed that the rice plants have stomata greatest width occurs when the plant is given 4 kHz frequency sound waves, the waves at 06.00 - 09.00. 
Co-Authors Aslama A. Aslama A. Bagyo Yanuwiadi D. J. Djoko Herry Santjojo Didik R. Santoso Dina A Chusniyah Djoko H. Santjojo Hari Soepriandono Hexa A. Hexa A. Kuni Nadliroh Muhammad Nurhuda Nathania Nanasari Nindha A. Berlianti Rara Pramita Saiful Rahman Sama’ Iradat Tito Septiana K. Sari Unggul P. Juswono Unggul P. Juswono