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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Y. Marsono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Potensi Bekasam Bandeng (Chanos chanos) sebagai Sumber Angiotensin I Converting Enzyme Inhibitor Prima Retno Wikandari; Suparmo Suparmo; Y. Marsono; Endang S. Rahayu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.69

Abstract

Bekasam adalah produk fermentasi ikan. Bekasam diduga mempunyai aktivitas Angiotensin I Converting Enzyme (ACE) inhibitor, yang disebabkan oleh terbentuknya peptida ACE inhibitor hasil aktivitas proteolitik enzim endogenous ikan dan bakteri asam laktat. Penelitian ini bertujuan mengetahui perubahan populasi bakteri asam laktat, pH, total asam, perubahan protein terlarut dan besarnya aktivitas ACE inhibitor selama proses fermentasi bekasam. Jumlah populasi bakteri laktat meningkat dari 5,16 menjadi 8,15 log CFU/g, nilai pH menurun dari 6,5 menjadi 4,41 disertai dengan peningkatan total asam. Bekasam bandeng menunjukkan aktivitas antihipertensi sebesar 51,77% yang terbentuk pada hari ke 6, dan tidak menunjukkan aktivitas penghambatan pada proses fermentasi selanjutnya. Besarnya aktivitas ACE inhibitor berkorelasi dengan terjadinya kenaikan protein terlarut selama proses fermentasi bekasam.
Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Agnes Murdiati; Hastari Wuryastuty; Y. Marsono; Eni Harmayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2576

Abstract

Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.
Indeks Glisemik Kacang Buncis (Phaseolus vulgaris) dan Kacang Panjang (Vigna sesquipedalis) serta Uji Efek Hipoglisemiknya pada Tikus Sprague Dawley Y. Marsono; Priyanto Triwitono; Sri Kanoni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2879

Abstract

Glycemic index (GI) of foods has been suggested as an additional parameter in selecting foods for the diabetic patients in nutritional management. GI was measured as the area under the postprandial blood glucose curve for a food expressed as a percentage of the area after the consumption of reference food (bread). In this research two legumes were investigated including snap bean (Phaseolus vulgaris L.) and yardlong bean (Vigna sesquipedalis). The objectives of this research were (i) to determine the IG of the beans and (ii) to investigate the effects of these legumes on serum glucose concentration of alloxan-induced diabetic rats. Ten health and normal volunteers (not diabetic) were provided. The volunteers took an overnight fasting, blood were drawn in the morning and analyzed for serum glucose. Then they were given the test beans containing total carbohydrates equivalent to 25-g glucose to be consumed. Blood samples were drawn for glucose measurement every 30 minutes until 120 min after meal. Serum glucose was determined enzymatically and the glucose responses were drawn graphically to calculate the GI of the beans. These beans were evaluated for their hypoglycemic properties in alloxan-induced diabetic rats. They were fed modified AIN 93 diets contain 20% of energy derived from snap bean or yardlong bean for 28 days. Serum glucose concentrations were measured weekly. It was found that with white bread as the food standard the GI of the snap bean and yard long bean were 43 and 86, respectively. In 28 days of diet intervention, snap bean decreased the serum glucose level by 73% (from 212mg/dL to 56mg/dL). Similar effect were found for the yardlong bean. This bean can reduced serum glucose level about 73% (from 224mg/dL top 61mg/dL), but both were similar to control diet that decreased the serum glucose level about 72% .
Co-Authors Agnes Murdiati Endang S. Rahayu Eni Harmayani Hastari Wuryastuty Prima Retno Wikandari Priyanto Triwitono Sri Kanoni Suparmo Suparmo