Garuda - Garba Rujukan Digital

PROFIL GC-MS DARI EKSTRAK DAUN RHIZOPHORA APICULATA DARI PESISIR TELUK TOMINI, SULAWESI TENGAH DENGAN AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN Didit Kustantio Dewanto; Roni Hermawan; Muliadin Muliadin; Putut Har Riyadi; Siti Aisiah; Wendy Alexander Tanod
Jurnal Kelautan Vol 14, No 1: April (2021)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v14i1.8904

ABSTRACTMangroves are plants with good tolerance to salinity changes, developing a chemical defense system with pharmacological value. This study aimed to obtain a GC-MS profiles of Rhizophora apiculata mangrove leaves extract, which could scavenge DPPH radicals and inhibit the growth of Listeria monocytogenes, Salmonella typhimurium, and Pseudomonas aeruginosa. The research included sampling, extraction (maceration with MeOH:DCM), identification of chemical profiles using GC-MS spectra analysis, assaying for antibacterial activity (well diffusion method), and antioxidants (DPPH radical scavenging). Mangrove leaves sampling was carried out on Laemanta, Parigi Moutong, Central Sulawesi. Based on the leaves’ characteristics and tips, the types of roots, fruits, and flowers, the mangrove leave samples were identified as R. apiculata. The GC-MS profiles of R. apiculata leaves extract was dominated by mome inositol (75.6%). The antibacterial assay showed at a concentration of 100 mg/mL of R. apiculata leaves extract showed weak to strong antibacterial activity, with the inhibition zone diameter of P. aeruginosa (1.22 ± 0.39 mm), S. typhimurium (3.00 ± 1.20 mm), and L. monocytogenes (17.22 ± 1.26 mm). The leaves extract of R. apiculata showed antioxidant activity, with an IC50 value of 96.68 ± 0.68 µg/mL. Based on the results, the mangrove leaves of R. apiculata extracts showed potential antibacterial and antioxidant activity. This study indicated that R. apiculata from Central Sulawesi were potential mangroves to discover and develop antibacterial and antioxidant agents.Keywords: GC-MS, Listeria monocytogenes, Parigi Moutong, Pseudomonas aeruginosa, Salmonella typhimurium.ABSTRAKMangrove merupakan tumbuhan dengan toleransi baik terhadap perubahan salinitas, sehingga mengembangkan suatu sistem pertahanan kimia yang bernilai farmakologis. Penelitian ini bertujuan mendapatkan profil senyawa dengan GC-MS dari ekstrak daun mangrove Rhizophora apiculata yang dapat menangkap radikal DPPH dan menghambat pertumbuhan Listeria monocytogenes, Salmonella typhimurium, dan Pseudomonas aeruginosa. Penelitian meliputi pengambilan sampel, ekstraksi daun mangrove (maserasi dengan MeOH:DCM), identifikasi profil senyawa dengan analisis spektra GC-MS, pengujian aktivitas antibakteri (metode difusi sumur) dan antioksidan (penangkapan radikal DPPH). Pengambilan sampel daun mangrove dilakukan di pesisir Desa Laemanta, Kabupaten Parigi Moutong, Sulawesi Tengah. Berdasarkan karakteristik bentuk dan ujung daun, jenis akar, buah, dan bunga, sampel daun mangrove terindentifikasi Rhizophora apiculata. Profil GC-MS dari ekstrak daun R. apiculata didominasi oleh senyawa mome inositol (75,6%). Hasil pengujian antibakteri pada konsentrasi 100 mg/mL ekstrak daun R. apiculata menunjukkan aktivitas antibakteri yang lemah sampai kuat, dengan diameter zona hambat pada P. aeruginosa (1,22 ± 0,39 mm), dan S. typhimurium (3,00 ± 1,20 mm), L. monocytogenes (17,22 ± 1,26 mm). Ekstrak daun R. apiculata menunjukkan aktivitas antioksidan dengan nilai IC50 sebesar 96.68 ± 0.68 µg/mL. Berdasarkan hasil pengujian ekstrak daun mangrove R. apiculata menunjukkan aktivitas antibakteri dan antioksidan. Penelitian ini mengindikasikan bahwa R. apiculata asal Sulawesi Tengah menjadi mangrove yang potensial dijadikan sebagai agen antibakteri dan antioksidan.Kata Kunci: GC-MS, Listeria monocytogenes, Parigi Moutong, Pseudomonas aeruginosa, Salmonella typhimurium.

PENGGUNAAN EKSTRAK DAUN SIRIH (Piper betle Linn) UNTUK MENGHAMBAT BAKTERI Aeromonas hydrophila DAN TOKSISITASNYA PADA IKAN PATIN (Pangasius hypophthalmus) Siti Aisiah; Muhammad Muhammad; Anita Anita
Fish Scientiae Vol 1 No 2 (2011): Issue December-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (63.591 KB)

This research was aimed a finding the part of Piper betle Linn which had thebiggest resistance to Aeromonas hydrophila bacteria and to know the minimal concentrate which could obstruct the growth of A. hydrophila bacteria and to know effective concentrate toxicity of P. betle Linn to Pangasius hypophthalmus. Therandom sampling used proportionate stratified random sampling. In toxicity test, it had be done 4 treatment, which was given to fish, those were : A = fish was injected with 25 % concentrate of extract P. betle Linn leaves, B = fish was injected with 75% concentrate of extract P. betle Linn leaves, C = positive control (fish was injected with sterile aquadest), and D = negative control (fish wasn’t injected) and 3 trial.Sensivity result of A. hydrophila bacteria to P. betle Linn showed that the axtract of Piper betle Linn leaves-methanol had bigger activity than others. Depended on MICtest of the leaves P. betle Linn-methanol extract showed result that the extract had25% minimal bloked concentrate to A. hydrophila bacteria. The result of toxicity testof P. betle Linn 75% and 25% leaves was no mortality 50% of P. hypophthalmus.Water quality parameter during experiment like dissolving oxygen, pH, ammonia,CO2, and temperature were still in reasonable range for Pangasius hypophthalmus.

POTENSI TUMBUHAN BANGKAL (Nauclea Orientalis) UNTUK PENGENDALIAN BAKTERI Aeromonas Hydrophila Siti Aisiah
Fish Scientiae Vol 2 No 2 (2012): Issue December-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.051 KB)

The purpose of this study was obtained method of controlling diseases caused by Aeromonas hydrophila in fish farming is environmentally friendly. In this study the sensitivity of the test A. hydrophila, a minimum test konsentari inhibiting A.hydrophila (MIC test) and toxicity test on tilapia. The design used for toxicity tests iscompletely randomized design with 4 treatments it A = Fish injected with extracts bangkal concentration of 20 %, B = Fish injected with extracts bangkal concentration of 40 %, C = Fish injected with extracts of 80 % concentration bangkal, and D =Control ( fish not injected ), repeated 3 times. This treatment was obtained from theresults of the sensitivity test antibacterial bangkal inhibition and has the power to killmost of the bacteria against A. hydrophila is bangkal leaf extract with distilled watersolvent. MIC testing showed that the extract bangkal - distilled water has a minimum of 20 % inhibition of the bacterial activity A. hydrophila. Results of toxicity tests conducted on tilapia with a concentration of 20 %, 40 % and 80 % mortality of tilapia under 50 %. Haematological observation that eretrosit, leokosit, blood plasm, hematocrit and leokokrit in each treatment is still in the normal range. The water quality parameters, dissolved oxygen, pH, ammonia, CO2 and temperature can still support the normal life of tilapia.

PENGGUNAAN MEDIA AIR YANG BERBEDA UNTUK MENINGKATAN DAYA TETAS TELUR DAN KELULUSAN HIDUP BENIH IKAN GURAMI (Osphronemus gouramy lac.) Akhmad Murjani; Agussyarif Hanafie; Siti Aisiah; Ahmad Rijal Kamil
Fish Scientiae Vol 12 No 1 (2022): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/fishscientiae.v12i1.185

Menyedia benih dalam usaha budidaya ikan gurami (Osphronemus gouramy lac.) memegang peranan yang sangat penting. Kendala utama dalam usaha pembenihan ikan gurami di kolam adalah tingginya tingkat mortalitas, terutama pada saat telur dan larva. Penelitian ini bertujuan untuk meningkatkan persentasi daya tetas telur dan kelulusan hidup larva ikan gurami pada media sumber air pemeliharaan yang berbeda. Penelitian menggunakan RAL dengan 3 perlakuan dan 3 ulangan yaitu perlakuan A sumber air sumur, perlakuan B sumber air kolam, dan perlakuan C sumber air bioflok. Hasil penelitian menunjukkan daya tetas telur perlakuan air sumur (98,43%) meningkat 0,28% dibandingkan dari perlakukan air kolam (98,15%) dan perlakukan air sumur (98,43%) meningkat 12,26% dibandingkan dari perlakuan air bioflok (86,17%), perlakuan air kolam (98,15%) meningkat 11,98% dibandingkan dari perlakuan air bioflok (86,17%). Kelulusan hidup pada perlakuan air sumur (88,72%) meningkat 1,11% dibandingkan dari perlakukan air kolam (87,61%) dan perlakukan air sumur (88,72%) meningkat 27,13% dibandingkan dari perlakuan air bioflok (61,57%), perlakuan air kolam (87,61%) meningkat 26,02% dibandingkan dari perlakuan air bioflok (61,57%). peningkatan daya tetas telur dan kelulusan hidup benih ikan gurami dengan media air sumur lebih baik dibandingkan dengan media air kolam dan media air bioflok. Providing seeds in the cultivation of gouramy (Osphronemus gouramy lac.) plays an important role. One of the biggest obstacles in the hatchery of gouramy in ponds is the high mortality rate, especially at the time of eggs and larvae. This research was aimed to increase the percentage of egg hatchability and survival rate of gouramy larvae in different rearing water sources. The research used RAL with 3 treatments and 3 repetitions, they are treatment A from well water, treatment B from pond water, and treatment C from biofloc water sources. The results showed that the hatchability of eggs from well water treatment (98.43%) increased by 0.28% compared to that of pond water treatment (98.15%) and well water treatment (98.43%) increased by 12.26% compared to water treatment. biofloc (86.17%), pond water treatment (98.15%) increased by 11.98% compared to biofloc water treatment (86.17%). Life pass in well water treatment (88.72%) increased by 1.11% compared to pond water treatment (87.61%) and well water treatment (88.72%) increased by 27.13% compared to biofloc water treatment (61 ,57%), pond water treatment (87.61%) increased by 26.02% compared to biofloc water treatment (61.57%). The use of well water media increased egg hatchability and survival rate of gouramy compared to pond water media and biofloc water media

IMUNOGENISITAS ANTIGEN HEAT KILLED Aeromonas hydrophila STRAIN LOKAL DESA SUNGAI BATANG DAN MANDIANGIN TERHADAP IKAN LELE DUMBO (Clarias gariepinus) Alna Chairunisa; Olga Olga; Siti Aisiah
Fish Scientiae Vol 13 No 1 (2023): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/fishscientiae.v13i1.203

Penelitian ini bertujuan untuk menyeleksi dan mendapatkan antigen bakteri A.hydrophila strain lokal desa Sungai Batang dan Mandiangin Kalimantan Selatan yang bersifat imunogenik terhadap ikan lele dumbo. Antigen diinaktif menggunakan metode pemanasan (heat killed). Rancangan penelitian ini menggunakan Rancangan acak lengkap (RAL) dengan 6 perlakuan 3 ulangan yang meliputi perlakuan vaksinasi dengan antigen Aeromonas hydrophila strain P5.1 (A), strain AM01 (B), strain P1.1 (C), strain P3.1 (D), strain P4.1 (E) dan kontrol diinjeksi dengan PBS pH 7,0 (K). Antigen diinjeksi secara intramuscular sebanyak 0.1 ml (kepadatan bakteri 109 sel/ml) per ekor ikan uji (A,B,C,D,E). Hasil uji imunogenisitas antigen strain lokal P5.1 dan P1.1 bersifat lebih imunogenik terhadap lele dumbo dibandingkan dengan antigen lainnya berdasarkan kemampuannya meningkatkan antibodi pasca vaksinasi. Uji reaksi silang antigen A. hydrophila strain lokal AM01, P1.1 dan P5.1 mampu bereaksi silang dengan antigen lainnya. Antigen P1.1 mampu mengenali antigen lebih banyak dibandingkan P5.1 dan AM01, sehingga dapat direkomendasikan menjadi kandidat vaksin. This study aims to select and obtain antigens of the local strain A. hydrophila bacteria in Sungai Batang and Mandiangin villages, South Kalimantan, which are immunogenic to African catfish. Antigen is inactivated using the heating method (heat killed). The design of this study used a completely randomized design (CRD) with 6 treatments and 3 replications which included vaccination with the antigen Aeromonas hydrophila strain P5.1 (A), strain AM01 (B), strain P1.1 (C), strain P3.1 ( D), strain P4.1 (E) and control were injected with PBS pH 7.0 (K). Antigen was injected intramuscularly as much as 0.1 ml (bacterial density 109 cells/ml) per test fish (A, B, C, D, E). The results of the antigen immunogenicity test of local strains P5.1 and P1.1 were more immunogenic to African catfish compared to other antigens based on their ability to increase post-vaccination antibodies. Antigen cross-reaction test A. hydrophila local strains AM01, P1.1 and P5.1 are capable of cross-reacting with other antigens. P1.1 antigen is able to recognize more antigens than P5.1 and AM01, so it can be recommended as a vaccine candidate.

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search