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All Journal Buletin Veteriner Udayana BERITA BIOLOGI
I Putu Agus Hendra Wibawa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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UJI DAYA HAMBAT EKSTRAK DAUN KEDONDONG (LANNEA GRANDIS ENGL) TERHADAP PERTUMBUHAN BAKTERI ERWINIA CAROTOVORA I Wayan Sudira; I Made Merdana; I Putu Agus Hendra Wibawa
Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.839 KB)

Abstract

Research with the title of the inhibition test kedondong leaf extract (Lannea grandisENGL)on the growth of bacteria Erwinia carotovora, causes soft rot of Aloe vera have beenconducted in vitro on media PPGA and testing of antibacterial activity of leaf extractkedondong on aloe vera leaf pieces. This study aims to determine the bactericidal activityof leaf extract and concentration kedondong minimal constraints on the growth ofbacteria Erwinea carotovora. Bacteria E. Carotovora isolated from the Aloe Vera plant softrot disease.Sections of leaves among the sick and the healthy cut to the size of ± 3 cm andcleaned with water and then soaked with 70% alcohol for 2 minutes inserted into laminar flow. Leaves clean kedondong have weighed as much as 100 grams chopped, then addedwith 1000 ml of methanol solvent. Immersion extract was filtered with filter paper watmanNo. 2.The filtrate obtained was evaporated by vacuum rotary evaporator to separate thesolvent (methanol) and the extract, made up to concentration. Bacteria isolated on mediapropagated PPGA tilted as stock for testing bacterial pathogens isolated colony of yellowwhitecolor with the aroma of sulfur-like smell of gas.Based on research results, it can beconcluded that the leaf extract could inhibit bacterial growth kedondongE carotovora withdoses at least 4% concentration in media PPGA and pieces of aloe vera leaves, whereas atconcentrations of 1, 2 and 3% have not been able to provide inhibition.
STUDI POTENSI TANAMAN TEBU IRENG (Saccharum officinarum L.) SEBAGAI ANTIOKSIDAN DAN ANTIMIKROBA Putri Sri Andila; I Putu Agus Hendra Wibawa; I Nyoman Lugrayasa; Wawan Sujarwo
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3924

Abstract

Tebu ireng (Saccharum officinarum L.) merupakan jenis tebu lokal yang memiliki ciri khusus yaitu warna batangnya yang hitam.Secara tradisional tebu ireng dimanfaatkan sebagai obat penyakit diabetes.Selain dapat dimanfaatkan secara tradisional sebagai obat diabetes, diyakini tebu ireng masih banyak menyimpan manfaat lain yang belum banyak diketahui. Studi ini dilakukan untuk mengetahui efektivitas ekstrak tebu ireng sebagai antioksidan dan antimikroba, ditinjau dari beberapa bagian tanamannya. Proses ekstraksi menggunakan metode maserasi dengan pelarut methanol, uji aktivitas antioksidan dilakukan dengan metode DPPH free radical scavenger menggunakan spektrofotometer UV-Vis. Pengujian aktivitas antimikroba dilakukan dengan menggunakan metode difusi agar (Kirby-Bauer disc diffusion method). Hasil studi menunjukkan bahwa tebu ireng memiliki kemampuan sebagai antiokidan.Tingkat kepekatan warna dari tebu ireng berkorelasi dengan aktifitas antiokidannya.Ekstrak dari keseluruhan bagian tanaman tebu ireng efektif untuk menghambat pertumbuhan bakteri Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa dan Streptococcus mutans, namun tidak efektif menghambat pertumbuhan jamur Candida albicans.Tebu ireng mengandung pigmen Antosianinserta kaya akan serat pangan .
Co-Authors I Made Merdana I Nyoman Lugrayasa I Wayan Sudira Putri Sri Andila Wawan Sujarwo