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E. Kurnianto
Faculty of Animal and Agricultural Sciences, Diponegoro University Tembalang Campus, Semarang 50275
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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INHERITANCE OF COAT COLOR OF KEJOBONG GOAT IN PURBALINGGA REGENCY, CENTRAL JAVA, INDONESIA T. Permatasari; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 3 (2014): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.3.137-143

Abstract

The objective of this research was to examine the inheritance of coat color pattern of Kejobonggoat. The material used was goat family with clear lineage, in which the number of samples were 130Kejobong family, consisted of 201 kids, 130 does and 51 bucks. Coat color of black, black white, blackbrown, brown, white brown and white, were observed. The coat color types were classified andgenetically grouped according to the pigmentation types and color patterns. Probability compilationgene of coat color of Kejobong goat were B-C-S-ii (black), -- cc -- -- (white), BBC-ssii (black white),BbC-ssii (black brown), bbC-ssii (white brown) and bbC-S-ii (brown). Inheritance of coat colorobserved and fenotipe ratio expected were used to calculate Chi-square. Results of study showed that theinheritance of coat color pattern of Kejobong goat were not in Hardy-Weinberg equilibrium withexeption of mating between black and black brown and mating between brown black and white brownshowing similarity in observations and expectations.
MILK PRODUCTION AND REPRODUCTIVE TRAIT CAUSED BY LOC514211 GENE MUTATION IN DAIRY COWS A. S. Anggraeni; E. Kurnianto; S. Johari; S. Sutopo; Z. Shujun
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.4.191-198

Abstract

The SNP (rs 42688595) in LOC514211 isuncharacterized gene and located in chromosome 13 in Bos taurus,hadsignificant effectson milk yieldand milk qualitythat have been identified from Chinese and British dairy cowsusing GWAS (Genome-wide Association Study) based on 60k SNP-Chipin the previous study. The objective of this study wasto confirmthe effects ofthe SNP in LOC514211onproductive traitsin another population of Chinese Holsteindairy cow. Four hundred and seventy dairy cows were use in this study,genomicDNA was extracted from the blood of dairy cow, PCR-RFLP wasapplied to genotype of these DNA samples. Analysis of Variance (ANOVA) was performed by the General Linear Model (GLM) procedure of SAS 9.13 to identifythe association of SNP with milk and reproductivetraits. Results showed that SNP inLOC514211was polymorphic in this herd of dairy cowand wasin Hardy-Weinberg disequilibrium,and had significant differencesamong genotype in milk yield, milk quality and reproductive traits, whichwere similar with the results of previous GWAS study based SNP-Chips. Inconclusion, indicated that thisSNP in LOC514211 might bepotential markersforboth milk and reproductivetraits.
Identification of BMP15 Exon 2 for fecundity traits by PCR-RFLP and nucleotide sequencies in Kejobong goat A. Febriana; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.220-226

Abstract

Kejobong goat is known as prolific and high productivity goat breed in Indonesia. PCR-RFLP and sequencing technique was established in the present study to accomplish the polymorphisms of Bone Morphogenetic Protein 15 (BMP15) gene exon 2 on Kejobong goat does. The blood samples was collected from 48 Kejobong does which were selected based on their litter size. The size of PCR amplification of BMP15 gene exon 2 was 837 bp. The product of PCR-RFLP technique digested by HinfI enzyme showed that the samples were monomorphic. Authentication result using nucleotide sequencing found 4 substitution (A391G, C464G, T828C and C830G), 1 alignment gap (site 817) and 1 insertion nucleotide (site 822). This mutations caused 6 haplotypes formatted. The mutants of BMP15 exon 2 on Kejobong goats indicated that this breed had their own mutation controling the prolific trait. The phylogenetic tree build on the sequences of BMP15 gene exon 2 of Kejobong goats was grouped into 3 clusters. The alignment gap indicated to be the specific marker for the prolific trait (duplet) in Kejobong goat. The particular insertion site could be the recognition site of Kejobong goat based on BMP15 exon 2.
POPULATION DYNAMICS ON ONGOLE GRADE CATTLE IN KEBUMEN REGENCY - CENTRAL JAVA J. Rohyan; S. Sutopo; E. kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 4 (2016): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.4.224-232

Abstract

The objectiveof this study was to elucidatereproductive characteristics, potential output and dynamics population of Ongole Gradecattle in Kebumen Regency, Province of Central Java. The studywasconductedfromSeptemberto November 2015.The materials used in the study were165 farmers as respondentshavingOngole Gradecattle. The research method used was survey. Respondent samples were taken from five districts in which the location was determined by using purposive sampling. Data were collected through direct interviews with livestock farmers as respondents. The analysis used wasdescriptive analysis. The results showed that the age at first mating was 26.87 month, S/C was 1.64, post-partum mating was4.52 month, weaning age was 3.97 month, calving interval was 14.32 month, and the birth rate topopulation was45.5%. Reproductive efficiency value was 91.8%, natural increase was 44.68%, and net replacement rate of bull and heifer were 1,209% and 253%,respectively, andtotal output 44.11%. Population dynamics of Ongole Gradecattle from 2009 to 2014 fluctuated with average growthwas -4.84%. In 2019, cow population of Ongole Gradewasestimated about 68.381 heads. In conclusion Kebumen Regencyhasa relatively high potential output of Ongole Gradecattle thatis44.11% and population dynamics from 2010 to 2014 fluctuated with average of decrease growth is 4.84%. Estimation of cow’s population of Ongole Grade cattle willincrease 16.7% per year. 
Polymorphism of β-lactoglobulin (β-LG)|SacII gene and its association to milk protein and milk production in Saanen goats R. Ambarwati; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.346-355

Abstract

The objectives of this study were to analyze and identify the β-LG genotype and examine its association with milk protein and milk production of Saanen goats. Materials used in this study were DNA that originated from 22 Saanen does and its milk protein and milk production recording that was taken at 2,5; 3,0; 3,5 and 4,0 years old of Saanen does. DNA was analyzed using PCR-RFLP method with SacII restriction enzyme to identify genotype of B-LG gene and then were sequenced for each genotype. Sequencing data was analyzed using Clustal W in MEGA7.0, while association of B-LG gene genotype with milk protein and milk production was analyzed using General Linear Model (GLM) in SAS 9.0. The results showed that two alleles (A and G), forming two homozygous genotypes (AA and GG) and one heterozygous genotype (AG) were found, but there were no significant different within genotype on milk protein and milk production. In conclusion, there was no association between β-LG genotype and milk protein and milk production of Saanen goats.
Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats D. A. Lestari; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.135-146

Abstract

The objectives of study were to identify and assess the amino acid diversity of Cytochrome b (Cyt b) gene, genetic marker and characteristic of specific amino acid in Kacang and Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa Grade goat (EG) were purposively sampled. The genomic DNA was isolated by Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with CytbCapF and CytbCapR primers and was sequenced. The results showed that there were two specific amino acids that distinguish KG and EG goat with C. hircus and C. aegagrus and four specific amino acids that distinguish KG and EG goat with C. falconeri, but there were no specific amino acids can be used as a genetic marker to distinguish between Kacang and EG goat. In conclusion, specific amino acids in Cyt b gene can be used as a genetic marker among KG and EG goat with 3 goat others comparator.
GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON MITOCHONDRIAL DNA D-LOOP SEQUENCE M. F. Harlistyo; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.204-209

Abstract

This study was aimed to find out the diversity of mtDNA D-loop at Kejobong goat. The completemtDNA D-loop sequence of 12 goat blood samples were analyzed from 4 different location inPurbalingga Regency, Central Java province, sub-districts Kejobong, Pangadegan, Bukateja, andKaligondang. The mtDNA D-loop was extracted from blood sample. DNA obtained were amplified byPCR (Polymerase Chain Reaction) method using primers (5’-tcactatcagcacccaaagc-3’) as forward and(5’-ggcattttcagtgccttgct-3’) as reverse and subsequently sequenced. After nucleotide sequencing analysisconducted, 548 bp along was obtained. Nucleotides were then aligned with Capra hircus (GenBankAccess No.: KF952601.1) and apparently there were 11 different sites on the segment of mtDNA Dloop.Five sites could be used as a specific marker to distinguish between the Capra hircus andKejobong goat, namely at the site of 317 (A-G), 403 (T-C), 434 (T-C), 537 (C-T), and 553 (A-G).Nucleotide sequence analysis also contained seven different haplotypes. It was concluded that thedistribution of the different sites showed different haplotype patterns in Kejobong goat.
Co-Authors A. Febriana A. S. Anggraeni D. A. Lestari J. Rohyan M. F. Harlistyo R. Ambarwati S. Johari Sutopo Sutopo T. Permatasari Z. Shujun