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All Journal AGRIVITA, Journal of Agricultural Science Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal BioEksakta : Jurnal Ilmiah Biologi Unsoed
Murni Dwiati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Journal : BioEksakta : Jurnal Ilmiah Biologi Unsoed

 1 
Penggunaan Marka RAPD Sebagai Penduga untuk Membedakan Jenis Kelamin pada Kantung Semar Nepenthes Gymnamphora Koleksi Kebun Raya Baturraden Dini Rizki Pertiwi; Murni Dwiati; Agus Hery Susanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.144 KB) | DOI: 10.20884/1.bioe.2019.1.2.1778

Abstract

Nepenthes gymnamphora is an endemic pitcher plant species in Java Island and one of the plant collections of Baturraden Botanical Garden. N. gymnamphora is a dioecious plant and its sex cannot be differed in vegetative development. Conservation efforts can be carried out more efficiently when sex identification is performed earlier thus leading to effective development of particular sexes. Random Amplified Polymorphic DNA (RAPD) technique is one molecular approach that can be employed in early identification of N. gymnamphora sex. The aims of this study are to assess whether there is difference of RAPD patterns between male and female N. gymnamphora and to find out how the difference is. Explorative method was applied to this study involving five N. gymnamphora individuals of Baturaden Botanical Garden collection of different sexes as samples. Genomic DNAs were extracted from youngest leaves of the five samples (two males, two females and one individual of unknown sex) and then used as templates to amplify RAPD markers. Five random primers were used in the amplification, i.e. OPK-16, OPP-15, OPA-15, OPP-08, and OPD-20. Two primers, i.e. OPP-08 (5’-ACATCGCCA-3’) and OPD-20 (5’-ACTTCGGCCAC-3’), produced RAPD bands of approximately 300 bp in males and sexually unknown individual. These bands did not appear in females, so that it can be presumably related to sex determination genes in N. gymnamphora. Primer OPP-08 also produced RAPD bands of approximately 250 bp in females individual. These bands did not appear in males, so it can be presumably related to sex determination genes in N. gymnamphora.
Induksi Kalus Nepenthes mirabilis(Lour.) Druce Menggunakan NAA danTDZ Nina Nurussakinah; Murni Dwiati; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4242

Abstract

Nepenthes sp. has a characteristic in the form of a modified leaf tip into a pitcher. Nepenthes mirabilis (Lour.) Druce in its original habitat is widely hunted for sale. Propagation of N. mirabilis in nature has very little success rate, to overcome this it is necessary to propagate it by means of in vitro culture and use young leaf explants to form callus. Growth regulators such as Auxins (NAA) and Cytokinins (TDZ) can stimulate callus growth. Auxins can stimulate callus formation by stimulating cell expansion and cytokinins can stimulate the cell division process. The purpose of this study was to determine the effect of NAA and TDZ in inducing N. mirabilis callus and to determine the interaction between NAA and TDZ in inducing N. mirabilis callus. The experimental design used was a factorial CRD pattern, with two factors, namely NAA and TDZ. NAA consists of four levels, namely, 0 mg / L (N0); 0.5 mg / L (N1), 1 mg / L (N2) and 1.5 mg / L (N3). TDZ also consists of four levels, namely 0 mg / L (T0); 1 mg / L (T1); 2 mg / L (T2) and 3 mg / L (T3). Each treatment was repeated three times. The results showed that the addition of NAA and TDZ had an effect on callus induction of N. mirabilis. The TDZ concentration of 1 mg / L (T1) stimulated callus induction, especially the percentage of callus formation and callus diameter. Meanwhile, NAA 1 mg / L (N2) boosted the percentage of N. mirabilis callus formation.
Co-Authors AGUS HERY SUSANTO Dini Rizki Pertiwi Iman Budi Santoso Iman Budisantoso Nina Nurussakinah Rochmatino Rochmatino Sulastri Anggorowati