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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Biologi Udayana Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Innovation and Sustainable Empowerment
Iman Budisantoso
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Veterinary

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In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D Budisantoso, Iman; Amalia, Nurul; Kamsinah, Kamsinah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11018

Abstract

The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of Vanda sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from Vanda sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/value was separated by F test at p < 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 < 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.
Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria Budisantoso, Iman; Indriani, Mona; Kamsinah, Kamsinah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.777 KB) | DOI: 10.15294/biosaintifika.v10i3.15718

Abstract

Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating  N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of  N. ampullaria microcutting. The purpose of this research was to determine the effect of  BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different)  test. The result showed that addition of BAP affected the growth of  N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at  0.57 ppm.
CADMIUM (CD) ABSORPTION AND PHENOL CONTENT IN POGOSTEMON EXPOSED TO HEAVY METALS Proklamasiningsih, Elly; Budisantoso, Iman; Kamsinah, Kamsinah
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.02

Abstract

Patchouli (Pogostemon cablin Benth.) is an important plant used by industrial facilities to absorb cadmium (Cd) in polluted land. We performed an experiment using plant medium polluted with both Cd and lead (Pb) with added humic acid. The aims of this study were to 1) determine the effects of humic acid in growth medium contaminated with Cd and Pb on the absorption of Cd and phenol content in patchouli, and 2) determine the Cd tolerance level of the growth media. A completely randomized factorial design was used for the experiment with two factors. The heavy metals were a combination of pure PbNO3 and Cd (PC) with a ratio 1 : 1, and included five concentrations: PC0 (without PbNO3 and without Cd); PC1 (250 ppm PbNO3 + 250 ppm Cd); PC2 (500 ppm PbNO3 + 500 ppm Cd); PC3 (750 ppm PbNO3 + 750 ppm Cd); PC4 (1,000 ppm PbNO3 + 1,000 ppm Cd) and humic acid concentration (0; 6,000; 12,000; and 18,000 ppm). Each treatment was replicated three times. The parameters observed were plant biomass, Cd absorption, and phenol content. The application rate of humic acid to the plant medium containing heavy metals influenced the growth of patchouli, Cd absorption, and phenol content. An application rate of 12,000 ppm of humic acid reduced the toxicity of the heavy metals and increased the dry biomass and phenol content of patchouli.
PENGARUH 2,4-D TERHADAP HIPOKOTIL DAN KOTILEDON KEDELAI VARIETAS SLAMET YANG DITUMBUHKAN SECARA IN VITRO Iman Budisantoso; Lucky Prayoga
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.816 KB)

Abstract

Tujuan yang akan dicapai dalam penelitian ini adalah mengetahui pengaruh 2,4-D terhadap pertumbuhan dan perkembangan hipokotil dan kotiledon kedelai varietas Slamet dalam kultur in vitro. Penelitian dilakukan secara eksperimental dengan rancangan percobaan Rancangan Acak Lengkap (RAL), perlakuan yang dicobakan adalah larutan 2,4-D yang terdiri dari 4 level konsentrasi yaitu 0 mg/L; 20 mg/L; 40 mg/L dan 60 mg/L dan masing-masing perlakuan diulang 4 kali. Parameter perkembangan eksplant yang diamati meliputi prosentase pertumbuhan melalui pembentukan kalus maupun proses embriogenesis, waktu tumbuh kalus, berat basah kalus dan jenis kalus yang terbentuk. Hasil penelitian menunjukkan bahwa eksplant hipokotil maupun kotiledon kedelai varietas Slamet yang ditumbuhkan dalam media MS dengan perlakuan 2,4-D 20-60 mg/L tidak menghasilkan kalus maupun tanaman melalui proses embriogenesis, eksplant berwarna coklat dan mati. Setelah perlakuan konsentrasi hormon diubah menjadi 0, 5, 10 dan 15 mg/L, kalus tumbuh pada eksplant hipokotil dengan perlakuan 2,4-D 5 mg/L, sedangkan eksplant kotiledon kalus tumbuh pada media dengan perlakuan 2,4-D 15 µM.
Respon Penambahan Asam Humat terhadap beberapa Kandungan Senyawa Aktif Pegagan (Centella asiatica) triani hardiyati; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3536

Abstract

The aim of this research is to know the respon of humic acid and some growth media on the contain of active biological substance in Centella asiatica (L.) Urb. Hopefully, through the application of humic acid in the combination of media, the growth and plant metabolism will improve to produce active biological substance as primar metabolism. The growth media used were : soil & sand (1 : 1) ; soil & husk (1 : 1 ) ; and soil & zeolite (1 : 2). The humic acid treatment were 4 concentration, 0 g/kg media, 4 g/kg media, 8 g/kg media and 12 g/kg media. The research method was Completely Randomized Design, with factorial as treatment design. The variable observed were alkaloid content, flavonoid content, the presence of terpenoid, and biomass weight. The research result showed that the high weight of biomass and alkaloid contain got in the addition of 8 g humic acid in media soil and sand. The high flavonoid contain got in addition of 4 g/kg media in media soil and zeolite. There was brawn ring in the extract analysis dissolved in chloroform, acetic acid, and strong sulfate acid.
Pertumbuhan Dan Kandungan Vitamin C Tanaman Kangkung Darat (Ipomoea reptans Poir) Pada Media Zeolit Dengan Penambahan Asam Humat Adhelia Syefanis; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.895 KB) | DOI: 10.20884/1.bioe.2019.1.2.1707

Abstract

Ipomoea reptans Poir is the most favoured vegetable that mostly known by the people because in addition having good nutrition content, it is one type of vitamin C good source other than fruits. Vitamin C is an antioxidant which is required by the body to maintain the immune of body from the damaging effect of free radicals. The addition of organic compound in the form of humic acid that has a complex structure with high molecular weight and contains an active group that can increase the growth and productivity of plants. The high availability of nutrients require a carrier such as zeolite. Zeolite is a silicate mineral which have a high cation exchange capacity (CEC) (varying between 80-180 meq/100g). The addition of zeolite as a carrier is expected to increase the production of food crops such as corn. This study aimed to know the effect of humic acid with zeolite growth media for the growth and vitamin C content in Ipomoea reptans Poir and also and to determine the concentration of humic acid that most effect to the growth and vitamin C content in Ipomoea reptans Poir. The research was conducted at the Laboratory of Plant Physiology and Greenhouse, Faculty of Biology, Jenderal Soedirman University, Purwokerto by using experimental methods. The research was conducted from January 2019 to March 2019 with using completely randomized design (CRD). The parameters were observed in this study are the number of leaves, plant fresh weight, dry weight of plants, and vitamin C content. The data were analyzed by analysis of variance at the level of 95% and 99%, then following by analytical analysis BNT 95%. The results of this research showed that the addition of humic acid with a concentration of 4 g.kg-1 zeolite growing media can increase Ipomoea reptans Poir growth and the addition of humic acid to the zeolite growth media cannot increase the vitamin C content of Ipomoea reptans Poir.
Pengaruh Asam Humat pada Media Tanam Zeolit terhadap Pertumbuhan dan Kandungan Vitamin C Sawi Hijau (Brassica juncea) Istiq Fauziah; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.186 KB) | DOI: 10.20884/1.bioe.2019.1.2.1669

Abstract

Green mustard (Brassica juncea) as one of the commonly consumed vegetables and acts as one of the main sources of C vitamin in addition to fruits. C vitamin is needed for the body because it has antioxidant. Humic acid is a component of organic acid soil which can increase the production of food crops and plantations. Zeolites have a hollow structure that allows use as a carrier of humic acid. Aside from being a career zeolite which is applied with humic acid can act as an ameliorant material, this is useful in increasing crop production. The objectives of this study are to determine the effect of humic acid with zeolite planting media on the growth and content of C vitamin on green mustard plants and determine the concentration of humic acid which most influences the growth and content of C vitamin in green mustard plants. The research was conducted at the Laboratory of Plant Physiology and Green House, Faculty of Biology, Jenderal Soedirman University, Purwokerto using an experimental method. The study was carried out from January to February 2019 using an experimental design namely Completely Randomized Design (CRD). The parameters were the number of leaves, wet weight and dry weight of roots, stems, leaves and C vitamin. The data obtained will be analyzed by analysis of variance at the confidence level of 95% and 99%, if the results are significantly different then continued with LSD at 95%. The results showed that application of humic acid in zeolite planting media had a very significant effect on growth and C vitamin content of green mustard greens. Humic acid with a concentration of 4 g.kg-1 is the most efficient concentration in increasing growth and C vitamin content of green mustard greens.
Kandungan Bahan Aktif Fomesafen dalam Biji Kedelai dan Kacang Hijau dari Tanaman yang Ditumbuhkan pada Tanah Bekas Paparan Reflex Murni Dwiati; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.78

Abstract

A research has been conducted to understand the responses of soybean and mungbean to the persistence of Reflex residuals in soils referring to its active substance contents, i.e. fomesafen, in the plants grown in Reflex-exposed soils. The study was carried out experimentally in glass house from June to November 2003 employing Completely Randomized Design (CRD) with treatments arranged factorial. Two factors were used, the first of which was plant species (soybean and mungbean) while the second was Reflex concentrations (0, 25, 50, 100, and 200 ppm). Each treatment combination was replicated three times. The parameters examined comprised of fomesafen contents in the seeds, number of seeds per pod,  weight of 100 seeds, and weight of total seeds. To measure fomesafen contents in the seeds, Gas Liquid Chromatograph with ECD detector and column containing 10% OV-101 chromosorb WHF 80/100 mesh of 2m lengths and 3 mm diameter was applied. Fomesafen residuals in the soils of 5 month persistence had no harmful effect on both soybean and mungbean plants. As well, they had no significant effect on the production variables of both plants. Soybean seeds planted in the 5-month Reflex-exposed soils still contained fomesafen while the corresponding mungbean seeds did not.
Pengaruh Bakteri Pelarut Fosfat Dan Bakteri Penambat Nitrogen terhadap Pertumbuhan Tanaman Tomat pada Tanah Masam Yenni Widi Astuti; Lestanto Unggul Widodo; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.138

Abstract

Acid soil is the dried soil which has great potential for growing of agriculture commodity but this kind of soil has not been used optimally. The acid soil which has acidity reaction at pH<5,0 has Al poisonous potency, less of organic material and sensitivity toward erosion, and also less of nitrogen (N) and phosphor (P). Those problems can be solved by doing phosphate-solubilizing bacteria and nitrogen fixing bacteria inoculation. The aims of this study were to know the effect of phosphate-solubilizing bacteria, nitrogen fixing bacteria, and the mixture of both of them to the wet weight, height, level of nitrogen and phosphor of tomato plants. This research was conducted experimentally for six weeks by using completely randomized design (CRD) with 4 treatments. One treatment is a treatment without bacteria inoculation and the other treatments used bacteria inoculations which were phosphate-solubilizing bacteria inoculation, nitrogen fixing bacteria inoculation, and the mixture of both of them inoculations. Data were analyzed by using analysis of variance (ANOVA) at the 95% and 99% confidence level. The result proved the mixture of phosphate-solubilizing bacteria and nitrogen fixing bacteria is the most effective inoculation to improve the wet weight, height, level of nitrogen, and level of phosphor of tomato plant in acid soil.
The Effect Of Date Palm Extract (Phoenix dactylifera L.) On Orchid Plantlets (Vanda tricolor Lind) Growth using In Vitro Culture Resita Nursechan; kamsinah kamsinah; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4227

Abstract

Vanda tricolor Lindl. is widespread in Java, Bali, and Sulawesi, V. tricolor on natural habitat became scarce due to forest destruction and natural disasters and human activities. To prevent the extinction it can be done by the propagation of orchids using in vitro culture. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates to increase growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so there are 18 trial units. The source of the explants used was orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.
Co-Authors Adhelia Syefanis Elly Proklamasiningsih ELLY PROKLAMASININGSIH Eming SUDIANA, Eming Imam Widhiono Indriani, Mona Istiq Fauziah Juwarno, Juwarno Kamsinah Kamsinah Kamsinah Kamsinah Kartika Sari, Lilik Khoerunnisa ., Khoerunnisa Lestanto Unggul Widodo Lucky Prayoga Murni Dwiati Murni Dwiati Nina Nurussakinah Nurfithri Hashifah, Fathimah Nursechan, Resita Nurul Amalia Nurussakinah, Nina Puspitadewi, Nur Aini Kurnia Rahmawati, Aprilia Resita Nursechan Retna Utarini Suci Rahayu, Diana triani hardiyati Triani Hardiyati Yenni Widi Astuti