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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati AGRIVITA, Journal of Agricultural Science
S. M. Widyastuti
Pusat Studi Pengendalian Hayati Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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THE INFECTION PROCESS OF Fusarium subglutinans IN Pinus merkusii SEEDLINGS Widyastuti, S. M.; Christita, M.; Harjono, Harjono; Christanti, S.
AGRIVITA, Journal of Agricultural Science Vol 36, No 2 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Pinus merkusii or tusam is an original Indonesian plant and it is naturally distributed in Aceh and North Sumatra. Damping-off disease is the main problem in its nurseries. Fusarium subglutinans is one of the leading causes of damping-off disease. The knowledge of fungal infections process of tusam seedlings is essential to control damping- off disease effectively.The aim of this research is to understand (1) infection process of F. subglutinans in tusam seedlings and the defence response of seedlings against the infection of F. subglutinans. The methods used in this research were (1) identification of fungal pathogens that causing the disease, (2) pathogenicity test of F. subglutinans, (3) detection the accumulation of lignin, accumulation of callose and hypersensitive reactions by staining of seedling tissue using phloroglucinol, aniline blue and lactophenol trypan blue.The results of this study revealed that spores germination occurred in two days after inoculation. Direct penetration through cell wall and stomata was observed on the third day after inoculation. There was hypersensitive reaction in stomata. Accumulation of callose and lignin appeared on the third day after inoculation. However, defence response of seedlings was not effective, as F. subglutinans is a necrotroph fungus.Keywords: damping-off, Fusarium subglutinans, Pinus merkusii, tusam
Produksi Fitoaleksin pada Tusam (Pinus merkusii Jungh. et de Vriese) sebagai Respon Infeksi Fungi Mikorisa Widyaningsih, S.; Widyastuti, S. M.; Sumardi, Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.029 KB) | DOI: 10.24002/biota.v11i2.2621

Abstract

The experiment aimed to detect (1) mycorrhizal infection induced phytoalexins production of the root of tusam and (2) its activity in suppressing a dumping off fungi, Fusarium sp and Rhizoctonia solani. Production of phytoalexins was detected from the extracts of mycorrhizal root of old trees and 4, 6 and 8 week-old seedlings in alcohol using a UV-spectrophotometer. The antifungal activity of phytoalexins was studied by inoculating the pathogenic fungi into mycorrhizal seedlings of tusam. The results showed that the spectra of phytoalexins of extracted mycorrhizal and non-mycorrhizal roots ranged from 203.2 to 204.6 nm. Higher antifungal concentrations of antifungal compound was obtained from the root extracts of old trees and 4 week-old seedlings, indicated by the higher spectrophotometric absorbance (0.315 and 0.324 g-1 root ml-1 ethanol), compared to that extracted from non-mycorrhizal root of the same plant origin. Mycorrhizal roots significantly suppressed the development of Fusarium sp. and Rhizoctonia solani.
Co-Authors Harjono Harjono M. Christita S. Christanti Sumardi . Widyaningsih, S.