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All Journal Program Kreativitas Mahasiswa - Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education
Nael Huda Qonita
Faculty of Biology, Universitas Jenderal Soedirman, Indonesia
Agriculture, Biological Sciences & Forestry Education

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SCIENCE CLUB OF PURWOKERTO SEBAGAI SARANA MENINGKATKAN POLA PIKIR ILMIAH PELAJAR PURWOKERTO Mukti, Muhammad Dwiyan; Maulana, Rizki Fauzi; Wulandari, Rani; Qonita, Nael Huda
Program Kreativitas Mahasiswa - Pengabdian Kepada Masyarakat PKM-M 2013
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Nowadays, the existence of juvenile delinquency in Junior high level or even Senior high is uncontrollable. Whether it’s brawl or even another way of naughtiness. they don’t realize that its can destruct their future. Juvenile delinquency happens because there is no right place to express their creativity. Science Club (KIR), is a extracurricular that exist in senior high level. This extracurricular is focus on science, whether its exact or even social science. Science Club (KIR) in Regional level is very needed because it can be right place for teenager to interact with another student from different school and also minimalize juvenile delinquency.
Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning Kusbiyanto, Kusbiyanto; Soedibja, Petrus Hary Tjahja; Nuryanto, Agus; Qonita, Nael Huda; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9301

Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome b-AluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.
Co-Authors Agus Nuryanto Hendro Pramono Kusbiyanto Kusbiyanto, Kusbiyanto Muhammad Dwiyan Mukti Petrus Hary Tjahja Soedibja Rani Wulandari Rizki Fauzi Maulana