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Hartutiningsih-M. Siregar
Pusat Konservasi Tumbuhan Kebun Raya - LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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UJI ANTIBAKTERI Lasianthus (RUBIACEAE) SEBAGAI TUMBUHAN BERKHASIAT OBAT DAN UPAYA PERBANYAKANNYA Purwantoro, R. Subekti; Siregar, Hartutiningsih-M.; Sudarmono, Sudarmono; Praptiwi, Praptiwi
Buletin Kebun Raya Vol 13, No 2 (2010): Buletin Kebun Raya Vol. 13 (2) Juli 2010
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Lasianthus, a member of the family Rubiaceae has potential for medicinal plants. The aims of the research were to find out the potential antibacterial activity of Lasianthus leaf extract and to investigate the effectiveness of plant propagation by stems cutting. The species tested were Lasianthus laevigatus Blume, L furcatus (Miq.) Bremek and L. cf. obscurus Blume. The extracted leaves in n-hexana, ethyl acetate and methanol were tested in vitro against Staphylococcus aureus and Escherichia coil activities that grown on medium of Mueller-Hinton agar. The methanol extracts of each plant material showed significant antibacterial activities on S. aureus and E. coll. Antibacterial activity increased with increasing concentration of the extracts. Plant propagation by stems cutting were done as a first step of conservation effort for respective species. Stems cutting of L. laevigatus, L. purpureus, L. furcatus, L. acuminatus, L rhinocerotis. and L. cf. obscurus with length of 10 cm were applied with Rotoon F to stimulate root growth. The result of plant propagation research showed that only the stems cutting of L. cf. obscurus grew well although roots did not grow until 4 months.
UJI ANTIBAKTERI Lasianthus (RUBIACEAE) SEBAGAI TUMBUHAN BERKHASIAT OBAT DAN UPAYA PERBANYAKANNYA Purwantoro, R. Subekti; Siregar, Hartutiningsih-M.; Sudarmono, Sudarmono; Praptiwi, Praptiwi
Buletin Kebun Raya Vol 13, No 2 (2010): Buletin Kebun Raya Vol. 13 (2) July 2010
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lasianthus, a member of the family Rubiaceae has potential for medicinal plants. The aims of the research were to find out the potential antibacterial activity of Lasianthus leaf extract and to investigate the effectiveness of plant propagation by stems cutting. The species tested were Lasianthus laevigatus Blume, L furcatus (Miq.) Bremek and L. cf. obscurus Blume. The extracted leaves in n-hexana, ethyl acetate and methanol were tested in vitro against Staphylococcus aureus and Escherichia coil activities that grown on medium of Mueller-Hinton agar. The methanol extracts of each plant material showed significant antibacterial activities on S. aureus and E. coll. Antibacterial activity increased with increasing concentration of the extracts. Plant propagation by stems cutting were done as a first step of conservation effort for respective species. Stems cutting of L. laevigatus, L. purpureus, L. furcatus, L. acuminatus, L rhinocerotis. and L. cf. obscurus with length of 10 cm were applied with Rotoon F to stimulate root growth. The result of plant propagation research showed that only the stems cutting of L. cf. obscurus grew well although roots did not grow until 4 months.
Potensi Antibakteri Ekstrak Daun Lasianthus Terhadap Bakteri Pseudomonas aeruginosa Purwantoro, R. Subekti; Siregar, Hartutiningsih-M.; Sudarmono, Sudarmono; Agusta, Andria
Jurnal Jamu Indonesia Vol. 1 No. 3 (2016): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v1i3.19

Abstract

Penelitian ini bertujuan untuk mengetahui sifat antibiotik dari ekstrak tumbuhan Lasianthus terhadap aktivitas bakteri Pseudomonas aeruginosa secara in-vitro. Ekstrak yang diuji adalah daun Lasianthus laevigatus, L. furcatus, dan L. obscurus. Ekstraksi tumbuhan dilakukan secara maserasi bertingkat dengan menggunakan ekstrak n-heksana, etil asetat, dan metanol. Potensi eskstrak sebagai antibakteri diuji melalui penghambatan aktivitas bakteri P. aeruginosa dengan menggunakan metode kertas cakram pada media agar Mueller-Hinton. Hasil penelitian menunjukkan bahwa ekstrak metanol pada daun L. furcatus memperlihatkan daya hambat tertinggi dengan zona hambat 12 mm (konsentrasi 50 μg), 19.5 mm (konsentrasi 100 μg), dan 20 mm (konsentrasi 200 μg), diikuti oleh L. obscurus dengan zona hambat 11 mm (konsentrasi 50 μg), 13 mm (konsentrasi 100 μg), dan 18.5 mm (konsentrasi 200 μg), sedangkan penggunaan ekstrak n-heksana dan etil asetat daya hambatnya rendah di bawah 10 mm, terkecuali ekstrak n-heksana pada daun L. obscurus daya hambat tinggi dengan zona hambat 11 mm (konsentrasi 50 μg).
Co-Authors ANDRIA AGUSTA Praptiwi Praptiwi Praptiwi Praptiwi R. Subekti Purwantoro SUDARMONO SUDARMONO Sudarmono Sudarmono