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All Journal Biogenesis: Jurnal Ilmiah Biologi Jurnal Farmasi
Endang Sutariningsih Soetarto
Jurusan Biologi, Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Khamir Penghasil Indole-3-Acetic Acid dari Rhizosfer Anggrek Tanah Pecteilis susannae (L.) Rafin Lasmini, Titi; Sutariningsih Soetarto, Endang
Biogenesis Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Indole-3-acetic acid (IAA) is a growth hormone produced by plants. Several types of yeast has been reported to produce these hormones, and generally produce IAA in an higher amount than in plants. The purpose of this study is to get the yeast producing indole-3-acetic acid in the rhizosphere soil orchid Pecteilis susannae (L.) Rafin and to test the ability of yeast isolates to form IAA on medium L-tryptophan given as precursors. Yeasts were isolated and tested for their ability to produce IAA in vitro. The results showed that there are four potential yeast isolates that produce IAA which are isolates K2, U5, A3, and A1. Highest concentration of IAA was produced by A3 isolates grown on a medium containing GPB 200 ug / ml of L tryptophan in the amount of 56.262 g/ml.Keywords: Indole-3-acetic acid, rhizosfer yeast, Pecteilis susannae
Pembentukan Biofilm Pseudomonas aeruginosa pada Beberapa Media Cair Wahyudi, Didik; Sutariningsih Soetarto, Endang
Jurnal Farmasi (Journal of Pharmacy) Vol. 10 No. 2 (2021): Jurnal Farmasi (Journal of Pharmacy), October 2021
Publisher : Jurnal Farmasi (Journal of Pharmacy)

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Pseudomonas aeruginosa is a Gram-negative rod-shaped bacterium that is an opportunistic pathogen that is the main cause of nosocomial infections and is able to form biofilms on growth media, biofilms often make the treatment of infectious diseases more difficult. There are several types of bacterial growth media, compositions and brands. The purpose of this study was to determine the ability of P. aeruginosa to form biofilms on several liquid culture media. P. aeruginosa was isolated from clinical samples from hospitals, the liquid media used were nutrient broth, lactose broth, brain-heart infusion (BHI), luria bertani broth, and tripticase soy broth. The biofilm formation test used the microtiter plate culture technique method, the ability to form biofilms was measured based on optical density using a microtiter plate reader at a wavelength of 570nm, with 0.1% crystal violet staining, after 24 hours incubation at 37oC, with 8 replications. The results showed that P. aeruginosa had the ability to form biofilms in nutrient broth 0.926±0.081, lactose broth 0.521±0.041, BHI 1.283±0.031, luria bertani 1,301±0.043, and trypticase soy broth media 1.563±0.032. The highest biofilm formation was in trypticase soy broth, and the lowest was in lactose broth, while in BHI and luria farming media the biofilm formation abilities were equivalent. The conclusion of this study is that P. aeruginosa has different abilities in forming biofilms when grown on different liquid media.
Co-Authors Didik Wahyudi Titi Lasmini