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All Journal Alchemy Jurnal Penelitian Kimia
Windrawati Windrawati, Windrawati
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Chemical Engineering, Chemistry & Bioengineering Energy Environmental Science Materials Science & Nanotechnology

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BIOSYNTHESIS OF BIOSURFACTANT BY PSEUDOMONAS AERUGINOSA USING CASSAVA FLOUR INDUSTRIAL WASTEWATER AS MEDIA Suryanti, Venty; Hastuti, Sri; Handayani, Desi Suci; Windrawati, Windrawati
Alchemy Jurnal Penelitian Kimia Vol 10, No 1 (2014)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Biosynthesis of biosurfactant by Pseudomonas aeruginosa have been prepared using cassava flour industrial wastewater (manipueira) as medium. The optimum condition of the biosurfactans biosynthesis was obtained using media containing nutrient broth and manipueira without centrifugation (NBM) with 4 days fermentation. UV-Vis and FT-IR spectra indicated that the biosurfactant was a rhamnolipid containing hydroxyl, ester, carbocylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 576  mg/L and surface tension value of 0.045  N/m. The biosurfactant was able to decrease the interface tension and form emulsion with benzene, toluene, gasoline and palm oil. This biosurfactant showed w/o emulsion system.
Co-Authors Desi Suci Handayani Laksono Trisnantoro Venty Suryanti