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All Journal 3BIO: Journal of Biological Science, Technology and Management JOMPA ABDI: Jurnal Pengabdian Masyarakat
Eri Mustari, Eri
Jurusan Agroteknologi Fakultas Sains dan Teknologi UIN Sunan Gunung Djati Bandung Jl. AH. Nasution 105 Bandung 40614
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Immunology & microbiology Languange, Linguistic, Communication & Media Neuroscience Social Sciences Other

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Analysis of Quercetin from Allium cepa L. Skin Waste in Inhibiting α-Amylase: An Alternate Therapeutic Drug to Treat Diabetes Muthahhari, Muhammad Zaky; Qordhowi, Muhammad Hisyam; Bonardi, Mohammad Rafi; Sofiani, Tania; Simanjuntak, Samuel Balapradana; Mustari, Eri
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 1 (2025): Vol. 7 No. 1 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.1.3

Abstract

Type 2 diabetes is a chronic metabolic disease characterized by an increase in blood sugar due to insulin resistance as well as the decline of insulin production by the pancreas. Quercetin is a flavonoid secondary metabolite that could be utilized as an α-amylase inhibitor and can potentially be used as a therapeutic drug in type 2 diabetes treatment by inhibiting starch hydrolysis in the human body. This research aims to determine the quercetin yield of Allium cepa L. skin waste, evaluate the α-amylase inhibitory properties of the extracted quercetin, and delve into the molecular mechanism of α-amylase inhibition by quercetin. Methods that were used in this research consisted of sample collection and preparation, quercetin extraction, testing for quercetin content in extract by spectrophotometry and HPLC, as well as testing for α-amylase inhibition by quercetin. Quercetin extraction produced 1.52 g of dry extract, yielding 16.8 mg of extract per g of dry shallot skin. Spectrophotometric analysis yielded 3.37 mg of quercetin per gram of dried shallot skin, while HPLC yielded 3.99 mg/g. The shallot skin extract inhibited α-amylase with an IC50 of 348.2 µg/mL. In comparison, acarbose had a more potent inhibitory effect, with an IC50 of 237.4 µg/mL. Quercetin inhibits α-amylase by forming hydrogen bonds with the active sites of the enzyme (Asp197, Glu233, and Asp300).
Teknologi Pengolahan Limbah Organik Menjadi Produk Bermanfaat Guna Menunjang Pertanian Berkelanjutan di Desa Argo Mulyo Daerah Penyangga Ibu Kota Nusantara Dewi, Mustika; Mustari, Eri; Rahmawati, Noor
Jompa Abdi: Jurnal Pengabdian Masyarakat Vol. 4 No. 4 (2025): Jompa Abdi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Jompa Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57218/jompaabdi.v4i4.2194

Abstract

Pelatihan teknologi pengolahan limbah organik di Desa Argo Mulyo dirancang untuk meningkatkan kapasitas masyarakat dalam mengubah limbah organik rumah tangga dan pertanian menjadi produk bernilai guna, seperti meta-enzyme, pupuk organik cair (POC), dan pupuk kompos. Metode pelatihan mencakup teknik fermentasi, penggunaan mikroorganisme efektif (EM4), serta pengomposan skala rumah tangga dengan bahan lokal yang mudah diperoleh. Pendekatan ini bertujuan mengurangi penggunaan pupuk anorganik dan meningkatkan kesuburan tanah secara berkelanjutan, sehingga mendukung ketahanan pangan di wilayah penyangga ibu kota Nusantara. Pelatihan turut didukung oleh pihak desa dan melibatkan partisipasi aktif petani, yang menunjukkan peningkatan pemahaman dan praktik pengelolaan limbah organik yang ramah lingkungan serta produktif. Hasil pelatihan ketiga jenis pupuk berhasil diproduksi dengan kualitas yang baik menggunakan sumber daya hayati lokal (seperti limbah buah, sayuran, kotoran ternak, dan limbah daun pisang) yang sebelumnya kurang dimanfaatkan. Dengan adanya pelatihan ini diharapkan mampu meningkatkan kesadaran dan keterampilan masyarakat dalam pengolahan limbah organik, mendukung peningkatan kualitas lahan dan produktivitas pertanian secara berkelanjutan, mengurangi dampak negatif limbah organik terhadap lingkungan, serta meningkatkan kesejahteraan masyarakat desa melalui peningkatan produktivitas pertanian organik yang berkelanjutan.
Co-Authors Bonardi, Mohammad Rafi Mustika Dewi Muthahhari, Muhammad Zaky Noor Rahmawati Qordhowi, Muhammad Hisyam Simanjuntak, Samuel Balapradana Sofiani, Tania