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All Journal Bioedukasi: Jurnal Pendidikan Biologi Proceeding Biology Education Conference
Rico Hutama Sulistiyo, Rico Hutama
Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Pengaruh Teknik Sterilisasi dan Komposisi Medium terhadap Pertumbuhan Tunas Eksplan Sirsak Ratu Sulistiyo, Rico Hutama; Luthfiyyah, Zayyan; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Yuliana, Nuniek; Prasetyo, Endry Nugroho
Bioedukasi: Jurnal Pendidikan Biologi Vol 11, No 1 (2018): BIOEDUKASI: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.619 KB) | DOI: 10.20961/bioedukasi-uns.v11i1.19726

Abstract

Successful tissue culture of soursop plants requires proper sterilization techniques and hormone levels to produce good shoot growth. This study aims to determine the effect of sterilization techniques and medium composition of soursop explants, using factorial completely randomized design with 2 factors: sterilizing agents and medium composition, repeated 3 times. Hydrogen Peroxide, Mercury and Sodium Hypoclorite used as sterilizing agents. The medium composition used in this study consisted of 3 levels: MS 0, MS 0.5 ppm BAP + 0.05 ppm NAA and MS 1 ppm BAP + 0.1 ppm NAA. The best treatment is MS medium 0.5 ppm BAP + 0.05 ppm NAA with hydrogen peroxide sterilizing agent which produces the highest propagation level and lowest contamination level.
Pengaruh Teknik Sterilisasi dan Komposisi Medium terhadap Pertumbuhan Tunas Eksplan Sirsak Ratu Sulistiyo, Rico Hutama; Luthfiyyah, Zayyan; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Yuliana, Nuniek; Prasetyo, Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Successful tissue culture of soursop plants requires proper sterilization techniques and hormone levels toproduce good shoot growth. This study aims to determine the effect of sterilization techniques and mediumcomposition of soursop explants, using factorial completely randomized design with 2 factors: sterilizing agentsand medium composition, repeated 3 times. Hydrogen Peroxide, Mercury and Sodium Hypoclorite used assterilizing agents. The medium composition used in this study consisted of 3 levels: MS 0, MS 0.5 ppm BAP+ 0.05 ppm NAA and MS 1 ppm BAP + 0.1 ppm NAA. The best treatment is MS medium 0.5 ppm BAP + 0.05ppm NAA with hydrogen peroxide sterilizing agent which produces the highest propagation level and lowestcontamination level.
Co-Authors Dalimartha, Lengga Nurullah DALIMARTHA, LENGGA NURULLAH Hery Widijanto Luthfiyyah, Zayyan LUTHFIYYAH, ZAYYAN Setyo Nugroho Susilo, Buana SUSILO, BUANA Wiguna, Eko Chandra WIGUNA, EKO CHANDRA Yuliana, Nuniek YULIANA, NUNIEK