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Fatkhunisa Rahmawati, Fatkhunisa
Student of Biomedical Engineering S1 Study Program, Physics Department, Faculty of Science and Technology Universitas Airlangga
Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Rahmawati, Fatkhunisa; Mayasari, Dita Ayu; Adhitioso, Satrio; Putra, Alfian Pramudita; Kuntjoro, Eko Budi; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

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Abstract

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.
TUBERCULOSIS COUNTER (TC) AS THE EQUIPMENT TO MEASURE THE LEVEL OF TB IN SPUTUM Purwanda, Fendy; Fibriawan, Yufan; Sasmito, Dyar; Rahmawati, Fatkhunisa; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.604 KB)

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. This disease is the third killer disease aftercardiovascular diseases and respiratory diseases, and is also he number one killer disease in a group of infectious diseases. This is partly due to the late handling and a non real time detection, both of which will inhibit the therapy which yields a large numberof microorganisms in the body, and will eventually complicate the recovery. Based on this phenomenon, we offered an alternativesolution for detecting the sum of microorganism using Tuberculosis Counter, a tool used to count the number of Tuberculosis bacteria in the patients sputum. Technically, the patients sputum preparat was screened using the TCS230 color sensor that was able to filter the color of the preparat. Tuberculosis bacteria in the stained sputum Ziehl-Niellsen preparat was colored red, while the other was colored blue. By utilizing these optical phenomena, the TCS230 color sensor was supposed to filter the red color in the preparat. By using regression equation measurement, we gained the equation which then correlated the bit value as an output of the sensor with the number of Tuberculosis bacteria. Then, the digitalization process yielded the real time and accurate data of Tuberculosis bacteria.
TUBERCULOSIS COUNTER (TC) AS THE EQUIPMENT TO MEASURE THE LEVEL OF TB IN SPUTUM Purwanda, Fendy; Fibriawan, Yufan; Sasmito, Dyar; Rahmawati, Fatkhunisa; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.604 KB) | DOI: 10.20473/ijtid.v3i2.205

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. This disease is the third killer disease aftercardiovascular diseases and respiratory diseases, and is also he number one killer disease in a group of infectious diseases. This is partly due to the late handling and a non real time detection, both of which will inhibit the therapy which yields a large numberof microorganisms in the body, and will eventually complicate the recovery. Based on this phenomenon, we offered an alternativesolution for detecting the sum of microorganism using Tuberculosis Counter, a tool used to count the number of Tuberculosis bacteria in the patient's sputum. Technically, the patient's sputum preparat was screened using the TCS230 color sensor that was able to filter the color of the preparat. Tuberculosis bacteria in the stained sputum Ziehl-Niellsen preparat was colored red, while the other was colored blue. By utilizing these optical phenomena, the TCS230 color sensor was supposed to filter the red color in the preparat. By using regression equation measurement, we gained the equation which then correlated the bit value as an output of the sensor with the number of Tuberculosis bacteria. Then, the digitalization process yielded the real time and accurate data of Tuberculosis bacteria.
The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Rahmawati, Fatkhunisa; Mayasari, Dita Ayu; Adhitioso, Satrio; Putra, Alfian Pramudita; Kuntjoro, Eko Budi; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 1 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.895 KB) | DOI: 10.20473/ijtid.v5i1.209

Abstract

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.
Co-Authors Alfian Pramudita Putra Dita Ayu Mayasari, Dita Ayu Dyar Sasmito, Dyar Eko Budi Kuntjoro, Eko Budi Fendy Purwanda, Fendy Prihartini Widiyanti Satrio Adhitioso Yufan Fibriawan, Yufan