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All Journal Jurnal Litbang Industri Biopropal Industri
Yoyon Suyono, Yoyon
Baristand Industri Pontianak, Jl. Budi Utomo No. 41, Pontianak
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Identification and Characterization bacteria Pseudomonas on Metal Contaminated Soil indicated Suyono, Yoyon; Salahudin, Farid
Biopropal Industri Vol 2, No 2 (2011)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

The research goals to determine the species of bacteria that can live in soil containing metal as a source of biomaterial biosorben. The research was conducted through the stages of isolation and characterization by testing the morphology, growth and biochemistry. Two isolates which have characteristics similar to the Pseudomonas genus and species of Pseudomonas sp. Pseudomonas sp has the ability to grow in extreme conditions.
Determination of Pseudomonas Bacteria Species and Analysis of Phylogenetic Tree using Bioinformatics Method Suyono, Yoyon
Biopropal Industri Vol 1, No 2 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.66 KB)

Abstract

Bioinformatics is the application of information technology in the field of biology by organizing and analyzing genetic data from a database of DNA, RNA and proteins into meaningful biological information. One role of bioinformatics is to identify the unknown bacteria and analysis of phylogenetic relationships based on genetic information is relatively easy, quick and cheap.The research aims to determine species and analyzed the phylogenetic tree bacteria of the genus Pseudomonas in Bioinformatics.  Research conducted through the stages of isolation, testing and continuing 16S rRNA bioinformatics studies include, for studies using the Basic Local Alignment Similarity Search Tool (BLAST) from NCBI, Multiple Sequence Alignment (MSA) using Clustal W from the EMBL-EBI and visualization in a phylogenic tree using the Tree View software. Isolation is obtained 2 (two) isolates the code 251 and 252. Testing 16S rRNA sequences of nucleotides in Fasta format. BLAST analysis in GenBank database of identity obtained respectively 99%, E-value 0.0 and the value of bits score above 50, Pseudomonas aeruginosa strains MZA-85 and Pseudomonas sp. J16. Multiple sequence alignment (MSA) and visualization of tree phylogenetic homologous isolates of code 251 and has a kinship with the species Pseudomonas aeruginosa Isolate 29 and isolate the code 252 with a species of Pseudomonas sp. J16 with a score of 99% and 100%. Isolates obtained by including the genus Pseudomonas species, Pseudomonas aeruginosa and Pseudomonas sp that has a nitrogen-fixing ability and resistance to the herbicide.
Preliminary Studies with Filler Organoclay Nanocomposite Making for Packaging Suyono, Yoyon
Biopropal Industri Vol 3, No 2 (2012)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Clay from West Kalimantan is still used as a raw material in the ceramic industry and it doesn’t have spesific processes for the manufacture of other products. The objective of this study is to determine the effect of organoclay filler on the physical properties of tensile strength clay nanocomposite food packaging applications. The step for clay nanocomposite were produced by purification, milling, organoclay, clay nanocomposite. Clay nanocomposite were characterized by analysis cation exchange capacities (CEC), X-ray diffraction (XRD), particle size analysis (PSA), scanning electron microscopy (SEM), tensile strength test and elongation at break. The purified with hydrochloric acid can increase the value of CEC and remove of the impurities. High energy milling (HEM) technique are with a time of 2 hours gained 225.3 nm sized nanoparticles as raw material organoclay. The addition of 1% organoclay filler increases the tensile strength of nanocomposite clay, according grade 2 and is suitable for second packaging or  not directly contact to food.
Zinc Adsorption Kinetics Using Pseudomonas as Biomass Hidayat, Hidayati; Suyono, Yoyon
Biopropal Industri Vol 5, No 1 (2014)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Zinc adsorption kinetics using Pseudomonas aeruginosa and Pseudomonas sp. was conducted to determine the ability of biomass to adsorb Zinc, and also to get the biosorption equilibrium and kinetics. Adsorption process conducted in this study was using batch method with variables on the amount of biomass (0.01 g, 0.02 g, 0.03 g, 0.04 g and 0.05 g), adsorption time (30, 60 and 120 min) and pH (4 and 6). Zinc metal biosorption kinetics following the pseudo-second-order equation. The highest correlation coefficient obtained on the condition of 0.04 g biomass for both pH 4 and pH 6 was 0.999. The best Zinc metal adsorption equilibrium models obtained at pH 4 with contact time at 60 minutes. This heavy metal adsorption equilibrium models comply the Freundlich isothermal with a correlation coefficient (R2) of 0.973.
Biosorpsi Logam Zn Pada Limbah Sintetik Menggunakan Biomassa Campuran Pseudomonas aeruginosa dan Pseudomonas sp Hidayati, Hidayati; Lusiana, Uray; Suyono, Yoyon
Jurnal Litbang Industri Vol 3, No 2 (2013)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1379.009 KB) | DOI: 10.24960/jli.v3i2.627.85-90

Abstract

Zinc is one of the heavy metals that could be harmful for environment. This metal usually arises from industrial activities. Biosorption of zinc in synthetic waste was conducted using biomass mixture of Pseudomonas aeruginosa and Pseudomonas sp. This research aims to determine the zinc adsorption capacity of the biomass in synthetic waste water. Zinc biosorption was performed at pH 4, room temperature and stirring 800 rpm. Variation of contact time used was 30, 60 and 120 min; and the amount of biomass used was 0.01 g, 0.02 g, 0.03 g, 0.04 g and 0.05 g. The highest zinc biosorption capacity was obtained 25.43% at the time of 120 minutes and the amount of biomass used 0.01 g. The optimum condition for biomass biosorption and removal capacity based on the correlation between experimental data and mathematical models was obtained with the addition of 0.04 g of biomass with correlation coefficient (R ) 1 and 0,965 respectively.ABSTRAK Salah satu logam berat yang berbahaya dari hasil kegiatan industri adalah logam Zn (seng). Biosorpsi logam Zn pada limbah sintetik dilakukan dengan menggunakan biomassa campuran Pseudomonas aeruginosa dan Pseudomonas sp. Penelitian ini bertujuan untuk mengetahui kapasitas biomassa dalam mengadsorpsi logam Zn pada limbah sintetik. Biosorpsi logam Zn dilakukan pada kondisi pH 4, temperatur ruang dan pengadukan 800 rpm. Variasi waktu kontak dilakukan pada 30, 60 dan 120 menit  dan menggunakan jumlah biomassa 0,01 g, 0,02 g, 0,03 g, 0,04 g  dan 0,05 g. Kapasitas biosorpsi logam Zn tertinggi diperoleh sebesar 25,43% pada waktu 120 menit dengan jumlah biomassa 0,01 g. Kondisi optimum biosorpsi logam Zn berdasarkan korelasi antara data eksperimen dan model matematika diperoleh pada penambahan jumlah biomassa sebesar 0,04 g baik untuk kapasitas biosorpsi logam Zn maupun efisiensi removal logam Zn dengan nilai koefisien korelasi (R2) masing-masing adalah 1 dan 0,965.
Co-Authors Farid Salahudin, Farid Hidayati Hidayat, Hidayati Hidayati, Hidayati Uray Lusiana, Uray