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All Journal Majalah Obat Tradisional
Johnson Marimuthu Antonisamy, Johnson Marimuthu
Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, India
Health Professions Medicine & Pharmacology Public Health

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FLAVONOID PROFILE OF Clitoria ternatea Linn Ponnusamy, Selvamaleeswaran; Gnanaraj, Wesely Ebenezer; Antonisamy, Johnson Marimuthu
Majalah Obat Tradisional Vol 19, No 1 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.418 KB) | DOI: 10.14499/mot-TradMedJ19iss1pp%p

Abstract

The present study was aimed to reveal the flavonoid profile of Clitoria ternatea L leaves, stem and seeds using the HPTLC analysis. The powdered leaves, stem and seed samples were extracted with 150 mL of methanol for 8-12 h by using the Soxhlet apparatus. The Ethyl acetate-butanone-formic acid-water (5:3:1:1) was employed as mobile phase for flavonoids. The developed plate was sprayed with 1% ethanolic aluminium chloride as spray reagent and dried at 100°C in hot air oven for 2min. The plate was photo-documented at UV 366 nm and daylight using Photo-documentation chamber.The methanolic extract of stem, leaves and seeds of Clitoria ternatea showed the presence of 24 bands with 18 different Rf values with range 0.01 to 0.96.  Out of 24 bands, 10 bands with seven Rf values viz., 0.04, 0.10, 0.28, 0.37, 0.49, 0.65 and 0.85 were idenified as flavonoids, the other bands were noted as unknown metabolites. Developed HPTLC chromatogram of Clitoria ternatea methanolic extracts of vegetative and reproductive parts could be used efficiently for identification, and quality assessment of the plant in the pharmaceutical industries. These profiles may used as chemical marker to solve plant systematic problems.
Co-Authors Selvamaleeswaran Ponnusamy, Selvamaleeswaran Wesely Ebenezer Gnanaraj, Wesely Ebenezer