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In Vitro Toxicity Evaluation of Caffeine Imprinted Polymer (CAF-MIP) for Decaffeination Method on Normal Chang Liver Cells Hashim, Fatimah; Mehamod, Faizatul Shimal; Nawi, Naizatul Akmal
Makara Journal of Technology Vol. 21, No. 1
Publisher : UI Scholars Hub

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Abstract

Over consuming of caffeine is one of the factors to a few health problems such as insomnia, hypertension and cardiovascular disease. This preliminary study was conducted to evaluate the Caffeine-Imprinted Polymer (CAF-MIP) toxicity that was synthesized for a new alternative method for decaffeination. It is crucial to evaluate the toxicity of CAF-MIPas this product is potential to be used as complimentary with any drinks containing caffeine. In this study, the CAF-MIP toxicity potential was confirmed on Normal Chang Liver cell (NCLC) based on its IC50 value andacridine orange and propidium iodide (AO/PI) staining for mode of cell death observation.Proliferation assay was also conducted after 24, 48 and 72 hours at 30 μg/ml on NCLC and it showed that CAF-MIP promote NCLC growth as shown by at various concentrationof CAF-MIPincrease the percentage of NCLC viability. Observation under light microscopes on NCLC incubated wit CAF-MIP and NIP showed the normal, viable cell morphology, cuboidal and monolayer cell morphology and this can be seen with green fluorescence when view under fluorescence microscope. In conclusion, from this study, it is proved that the CAF-MIP does not initiate toxicity effects on human liver cells, meanwhile induction of cell proliferation was observed.
Chemical Constituents and Antifeedant Activity of Essential Oils from Four Selected Malaysian Local Plants against the Invasive Red Palm Weevil Larvae Tay, Karh Yan; Mehamod, Faizatul Shimal; Azmi, Wahizatul Afzan; Harun, Nor Omaima; Adnan, Azila; Zakeri, Hazlina Ahamad
Indonesian Journal of Chemistry Vol 26, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.97509

Abstract

Red palm weevil, a significant pest affecting oil palm cultivation, necessitates eco-friendly control strategies due to the environmental and health risks posed by synthetic insecticides. This study explores the efficacy of essential oils from lemongrass, gelam, pandan, and beach vitex as sustainable alternatives. Employing hydrodistillation for oil extraction, this study assessed the total phenolic content (TPC) and total flavonoid content (TFC) using gallic acid and quercetin standards, respectively. GC-MS analysis was conducted to identify the chemical constituents. The antifeedant activity was evaluated through food consumption, larval weight changes, and the feeding deterrent index (FDI) in sago food substrate experiments. Findings show oil yields of 0.42, 0.24, 0.04, and 0.03% w/w for lemongrass, gelam, beach vitex, and pandan, respectively, with gelam exhibiting the highest TPC and TFC (12.3 ± 0.36 and 10.8 ± 0.03). Significant constituents identified include beta-citral and citral in lemongrass, terpinolene in gelam, alpha-pirene in vitex, and phytol in pandan. Lemongrass and gelam displayed notable antifeedant effects, with FDI ranging from 24 to 28%, suggesting their potential as alternatives for managing the red palm weevil. This research highlights the potential of lemongrass and gelam oils as environmentally friendly and effective alternatives to synthetic insecticides in combating the invasive red palm weevil.