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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Jusup Subagja, Jusup
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Sistematik Numerik Strain-Strain Anggota Genus Pseudomonas Pendegradasi Alkilbenzen Sulfonat Liniar Berdasarkan Sifat Fenotip dan Protein Fingerprinting Suharjono, Suharjono; Sembiring, Langkah; Subagja, Jusup; Ardyati, Tri; Lisdiana, Lisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.138 KB) | DOI: 10.24002/biota.v12i1.2536

Abstract

Bacteria strains consisting of Pseudomonas sp. strain J and R isolated from river ecosystem polluted and Pseudomonas sp. strain A and B isolated from river ecosystem unpolluted by detergent were capable to degrade of LAS. The objective of this research was to determine similarity value by numerically of LAS-degrading Pseudomonas strains based on phenotype character and protein fingerprinting using three reference strains consist of Pseudomonas putida FNCC071, P. fluorescens FNCC070, and P. aeruginosa FNCC063. Phenotype characteristics examined are cellular and colony morphology, biochemical nature, capability to degrade polysaccharide, tolerance to various environmental factors and antibiotics, and ability to ferment sugar. Cellular protein fingerprinting was analyzed using SDS–PAGE discontinuous. Strains classification was determined based on Simple Matching Method similarity index by UPGMA (Unweight Pair Group Method with Average) algorithm. Based on phenotype nature, all strains have similarity value 0.61; however, based on cellular protein fingerprinting, those strains have similarity value 0.52. All strains of LAS-degraded were including in the genus of Pseudomonas.
Penggunaan Operkulum dalam Penentuan Umur pada Rhinoclavis sinensis Gmelin 1791 (Gastropoda: Cerithiidae) Zahida, Felicia; Subagja, Jusup
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.641 KB) | DOI: 10.24002/biota.v15i3.2601

Abstract

Age determination of the sample specimen is very important for population dynamic research of R. sinensis (Gastropoda : Cerithiidae). Operculum is a “hard” part stick on the dorsal portion of the foot of gastropods. Operculum appeared on the day of the snails are born and have their own specific shape, size, and materials composition. The aim of this research was to describe the use of operculum in age determination of R. sinensis. The method used in this research was: the operculums were soaked into saturated sodium hydroxide, washed and mounted them in Canada balsam, and observed using binocular microscope. Regression analysis was used to find the relationship between length of operculum to length of shell, and number of adventicious layers of operculum to length of shell. The research resulted that the length of operculum was comparable with the shell length, with the regression line of Y=0.113X +1.898 (R2=0.857). The growth of adventitious’ layers of the operculum coincided with the growth of the shell that in the second year, the growth of adventitious layers was two layers a year. At the age of three and four, the growth was one layer a year. During the fifth, sixth and seventh years, the growth was only a layer within three years. The regression line was Y=0.168X (R2=0.872).
Perilaku Spasio Temporal Tikus Sawah (Rattus argentiventer) Betina Hadi, Susilo; Subagja, Jusup; Sudarmaji, Sudarmaji
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.629 KB) | DOI: 10.24002/biota.v11i2.2630

Abstract

This research aimed to study spatio temporal behavior of the female ricefield rat, Rattus argentiventer, including home range, range spans, habitat use and daily activity. Twelve rats were released in two locations of a ricefield area from June 11th until July 24th, 2001. The movement of each rat was monitored using radio-tracking three times a day. The result was used for calculating home range size, range spans, habitat used and daily activities of female field-rats, using the computer program Ranges V. The results showed that the average female ricefield-rat’s home ranges and range spans were 0.84  0.16 Ha and 184.54  22.7m respectively. During daylight, the rats spent 82%, 16% and 2% of their activity living in dikes, in villages and in ricefield respectively. On the contrary, during the night 95%, 3% and 2% of the rats were active in the ricefield, villages and dikes respectively.
Co-Authors Felicia Zahida Jamhari Jamhari LANGKAH SEMBIRING Lisa Lisdiana SUDARMAJI SUDARMAJI Susilo Hadi, Susilo Tri Ardyati