Work stress is an emotional condition that experienced by employees as a result of workload. This not only impacts individuals but also impacts organizations. The purpose of this study to analyze the effect of workload on work stress mediated by work life balance. This research was conducted at the Department of Culture of Badung Regency with a sample of 82 contract employees selected using the saturated sampling method. Data were collected through interviews, observations, and surveys. Questionnaires were employed as the study tool. Path analysis using SPSS version 25.0 was the data analysis method employed. The study's findings demonstrate that workload significantly and favorably affects work-related stress. Work-life balance is negatively and significantly impacted by workload. Work life balance has a negative and significant effect on work stress. Work life balance is able to partially mediates the effect of workload on work stress. This study strengthen the Job Demands-Control Model theory in explaining work stress behavior and are expected to serve as a valuable reference for the Department of Culture of Badung Regency in managing work stress issues and formulating strategic policies.