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All Journal Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF APPLIED PHYSICS
Suharsono S, Suharsono
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Astronomy Biochemistry, Genetics & Molecular Biology Energy Immunology & microbiology Materials Science & Nanotechnology Physics

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Molecular cloning of gene fragment encoding 4-coumarate: Coenzyme A ligase of Sengon (Paraserianthes falcataria) Hartati, Sri N.; Sudarmonowati, Enny; S, Suharsono; Sofyan, Kurnia
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1105.206 KB)

Abstract

4-coumarate:Coenzyme A ligase (4CL) plays an important role in lignin biosynthetic pathway thatcatalyzed the activation of coumaric acid, caffeic acid or ferulic acid to be a syringil monomer. Ligninbiosynthesis control through 4CL down regulating would support lower lignin wood production. Theobjective of this study was to clone conserved region cDNA of gene encoding 4CL. Gene fragment isolation wasconducted by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerateheterologous primer. The RT-PCR products were purified, sequenced and analyzed to select the highlyhomologous fragment to 4CL. BLASTanalysis result showed that deduction of amino acid sequences from oneof two RT-PCR product nucleotide was highly homologous with the 4CL conserved region from Rubbus ideaus,Oryza sativa, Populus tomentosa, Populus balsamifera, Betulla platyphilla, Nicotiana tabacum, and Arabidopsisthaliana with identity ranging from 78-90%.Key words: 4-coumarate: Coenzyme A ligase, lignin, sengon
Measurement of Attenuation Coefficients of Near Surface Material Using SASW Method S, Suharsono
INDONESIAN JOURNAL OF APPLIED PHYSICS Vol 4, No 01 (2014): April
Publisher : Department of Physics, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/ijap.v4i01.1178

Abstract

The spectral analysis of surface waves (SASW) is an in situ non-destructive method. It has been developed and used for many years in the fields of geotechnical engineering and site characterization. It is typically used in evaluation of elastic moduli and layer thickness of soils, rocks and pavements.In this study the SASW was used to measure an attenuation coefficient (α) of near surface material at 15 development sites with different material e.i. soils, granite and methamorphics rock.. The attenuation coefficient which is the exponential decay constant of the amplitude of surface wavepropagation was approximated by Bornitz equation. The amplitude of vertical ground surface vibration generated by impact tests on the ground surface was measured at various radial distances.A good empirical correlation between the measured attenuation coefficients and the corresponding shear modulus and SPT or RQD data were established. These results suggest that the attenuation coefficient could be used as an alternative parameter for characterization of near surface material. Based on this method, the subsurface material at sites the study can be classified into two groups.The first group represented a low stiffness material with attenuation values of greater than 0.00516 s/m and the second group was a high stiffness material with attenuation values of less than 0.00150 s/m.However, this method of evaluation was still in development stage and needs to be substantiated with more results of measurements.
Co-Authors Enny Sudarmonowati Kurnia Sofyan Sri N. Hartati, Sri N.